Photosynthetica, 2020 (vol. 58), 1

Photosynthetica 2020, 58(1):80-86 | DOI: 10.32615/ps.2019.158

Nitric oxide participates in the regulation of ascorbate-glutathione cycle and water physiological characteristics of Arabidopsis thaliana by NaHS

C.J. SHAN1,2, Y.Y. JIN1, Y. ZHOU1,2, H. LI3
1 Henan Institute of Science and Technology, Xinxiang 453003, China
2 Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, China
3 School of Life Sciences, Henan University, Kaifeng 475004, China

We investigated the role of nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle and water physiological characteristics of Arabidopsis thaliana by sodium hydrosulfide (NaHS). NaHS markedly increased the contents of H2S, NO, chlorophyll (Chl), and carotenoids, the activity of AsA-GSH cycle, ascorbate/dehydroascorbate ratio, net photosynthetic rate, Chl fluorescence parameters, transpiration rate, stomatal conductance, and relative water content in leaves and the biomass of wild-type Arabidopsis. However, NaHS markedly decreased malondialdehyde content and electrolytic leakage. Except H2S, above NaHS-induced promotions were suppressed by nitrate reductase (NR) inhibitor sodium azide (NaN3). Application of sodium nitroprusside (SNP) to (NaN3+NaHS)-treated wild type Arabidopsis (NaN3+SNP+NaHS) reversed above effects of NaN3+NaHS. However, NaN3+NaHS and NaN3+SNP+NaHS had no significant effects on H2S content. Meanwhile, we proved above results by using NO-associated NR gene mutant nia1,nia2. Above results suggested that NO participated in the regulation of AsA-GSH cycle and water physiological characteristics of Arabidopsis by NaHS.

Keywords: donor; gas exchange; Halliwell-Asada pathway; hydrogen sulfide; nitric oxide; nitrate reductase gene mutant.

Received: April 18, 2019; Accepted: November 18, 2019; Prepublished online: December 20, 2019; Published: March 10, 2020Show citation

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SHAN, C.J., JIN, Y.Y., ZHOU, Y., & LI, H. (2020). Nitric oxide participates in the regulation of ascorbate-glutathione cycle and water physiological characteristics of Arabidopsis thaliana by NaHS. Photosynthetica, 58(1), 80-86. doi: 10.32615/ps.2019.158.
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