Photosynthetica, 2012 (vol. 50), issue 1

Photosynthetica 2012, 50(1):95-108 | DOI: 10.1007/s11099-012-0018-6

Fluorescence imaging of light acclimation of brazilian atlantic forest tree species

L. Dos Anjos1,*, M. A. Oliva1, K. N. Kuki1
1 Departamento de Biologia Vegetal, Universidade Federal de Viçosa (DBV/UFV), Viçosa, Brazil

In the pursuit of knowledge on the biological behavior of Brazilian Atlantic Forest tree species, this study evaluated the susceptibility of the light-demanding species, Schinus terebinthifolia Raddi., Pseudobombax grandiflorum (Cav.) A. Robyns and Joannesia princeps Vell., and of the shade-tolerant species, Hymenaea courbaril L. var. stilbocarpa and Lecythis pisonis Camb, to photoinhibition and acclimation capacity. These species were first cultivated under two irradiance conditions, I20 (20% direct sunlight radiation) and I100 (all-sky or direct sunlight) and then transferred from I20 to I100. The effects of the sudden increase in light radiation intensity on photosynthetic activity were then evaluated through chlorophyll (Chl) fluorescence imaging, HPLC xanthophylls analysis, and cell membrane lipid peroxidation measurements. Light-demanding species were found to present a higher photochemical efficiency and higher acclimation capacity under high light irradiance than shade-tolerant species. The higher photoinhibition tolerance observed in light-demanding species was associated to their higher capacity for photochemical dissipation and dissipation of excess excitation energy via the xanthophyll cycle, leading to a lower ROS generation. The obtained results suggested that a knowledge of acclimation capacity, by means of Chl fluorescence imaging yields, is a useful indicator of species successional grouping.

Keywords: chlorophyll fluorescence; light stress; photochemical efficiency; thermal dissipation; xanthophyll cycle

Received: June 8, 2011; Accepted: November 29, 2011; Published: March 1, 2012Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Anjos, L., Oliva, M.A., & Kuki, K.N. (2012). Fluorescence imaging of light acclimation of brazilian atlantic forest tree species. Photosynthetica, 50(1), 95-108. doi: 10.1007/s11099-012-0018-6.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ali, M.B., Hahn, E., Paek, K.: Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet. - Environ. Exp. Bot. 54: 109-120, 2005. Go to original source...
    2. Alves, P.L.C.A., Magalhães, A.C.N., Barja, P.R.: The phenomenon of photoinhibition of photosynthesis and its importance in reforestation. - Bot. Rev. 68: 193-208, 2002. Go to original source...
    3. Baker, N.R., Rosenqvist, E.: Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities. - J. Exp. Bot. 55: 1607-1621, 2004. Go to original source...
    4. Baker, N.R.: Chlorophyll fluorescence: a probe of photosynthesis in vivo. - Annu. Rev. Plant Biol. 59: 89-113, 2008. Go to original source...
    5. Barbosa, L.M.: [Forest restoration of degraded areas in São Paulo State: history, current situation and projects. - In: Barbosa L.M. (ed.): Manual for Restoration of Degraded Areas of São Paulo State - Riparian Forests of Inland São Paulo State.] Pp. 4-25. Instituto de Botânica, São Paulo 2006. [In Portuguese.]
    6. Barros, N.F., Brandi, R.M.: [Influence of three forest species over soil fertility of pasture in Viçosa, Minas Gerais State.] - Brasil Florestal 6: 24-29, 1975. [In Portuguese.]
    7. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77k among vascular plants of diverse origins. - Planta 170: 489-504, 1987. Go to original source...
    8. Bros, E., Meyer, S., Genty, B.: Heterogeneity of leaf CO2 assimilation during photosynthetic induction. - Plant Cell Environ. 19: 1349-1358, 1996.
    9. Burritt, D.J., Mackenzie, S.: Antioxidant Metabolism during Acclimation of Begonia x erythrophylla to High Light Levels. - Ann. Bot. 91: 783-794, 2003. Go to original source...
    10. Calatayud, A., Roca, D., Martínez, P.F.: Spatial-temporial variations in rose leaves under water stress conditions studies by chlorophyll fluorescence imaging. - Plant Physiol. Biochem. 44: 564-573, 2006. Go to original source...
    11. Carneiro, M.A.C., Siqueira, J.O., Davide, A.C., Gomes, L.J., Curi, N., do Vale, F.R.: Mycorrhizal fungi and superphosphate and the growth of tropical woody species. - Sci. Forest. 50: 21-36, 1996.
    12. Carpanezzi, A.A.: [Grounds for rehabilitation of ecosystems forest. - In: Galvão, A.P.M., Porfírio-da-Silva V. (ed.): Forest Restoration - Fundamentals and Case Studies.] Pp. 27-46. Embrapa, Colombo-PR 2005. [In Portuguese.]
    13. Carvalho, P.E.R.: [Brazilian tree species.] Vol 1 and 2. Embrapa, Colombo-PR, 2006. [In Portuguese.]
    14. Chen, X., Li, W., Lu, Q., Wen, X., Li, H., Kuang, T., Li, Z., Lu, C.: The xanthophyll cycle and antioxidative defense system are enhanced in the wheat hybrid subjected to high light stress. - J. Plant Physiol. 168: 1828-1836, 2011.
    15. Cheng, L.: Xanthophyll cycle pool size and composition in relation to the nitrogen content of apple leaves. - J. Exp. Bot. 54: 385-393, 2003. Go to original source...
    16. Delagrange, S., Messier, C., Lechowicz, M.J., Dizengremel, P.: Physiological, morphological and allocational plasticity in understory deciduous trees: importance of plant size and light availability. - Tree Physiol. 22: 775-84, 2004. Go to original source...
    17. Demming-Adams, B.; Adams, W.W., III: Photoprotection and other responses of plants to high light stress. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 43: 599-626, 1992. Go to original source...
    18. Demming-Adams, B.; Adams, W.W., III: The role of xanthophylls cycle carotenoids in the protection of photosynthesis. - Trends Plant Sci 1: 21-26, 1996. Go to original source...
    19. Demmig-Adams, B., Ebbert, V., Zarter, C.R., Adams, W.W., III.: Characteristics and species-dependent employment of flexible versus sustained thermal dissipation and photoinhibition. - In: Demming-Adams, B.; Adams W.W., III, Matoo, K. (ed.): Advances in Photosynthesis and Respiration - Photoprotection, Photoinhibition, Gene Regulation and Environment. Vol. 21. Pp. 39-48. Springer, Dordrecht 2006. Go to original source...
    20. Ebbert, V., Demmig-Adams B., Adams, W.W., III., Mueh, K.E., Staehelin, L.A.: Correlation between persistent forms of zeaxanthin-dependent energy dissipation and thylakoid protein phosphorylation. - Photosynth. Res. 67: 63-78, 2001. Go to original source...
    21. Ebbert, V., Adams W.W., III, Mattoo, A.K., Sokolenko A., Demmig-Adams B.: Upregulation of a PSII core protein phosphatase inhibitor and sustained D1 phosphorylation in zeaxanthin-retaining, photoinhibited needles of overwintering Douglas fir. - Plant Cell Environ. 28: 232-240, 2005. Go to original source...
    22. Einhorn, K.S., Rosenqvist, E., Leverenz, J.W.: Photoinhibition in seedlings of Fraxinus and Fagus under natural light conditions: implications for forest regeneration? - Oecologia 140: 241-251, 2004. Go to original source...
    23. Gandolfi, S.: [Light regime in semidecidual forests and its possible consequences. - In: Alfredo, Jún. (ed.): Brazilian Ecosystems: Management and Conservation.] Pp. 305-311. Expressão Gráfica e Editora, Fortaleza-Ceará 2003. [In Portuguese.]
    24. Genty, B., Briantais, J., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    25. Govindjee, Nedbal, L.: The chlorophyll fluorescence imaging and its application in plant science and technology - 3rd Regional Photosynthetic Workshop - Photosynthetica 38: 481-482, 2000. Go to original source...
    26. Gray, G.R., Hope, B.J., Qin, X., Taylor, B.G., Whitehead, C.L.: The characterization of photoinhibition and recovery during cold acclimation in Arabidopsis using chlorophyll fluorescence imaging. - Physiol. Plant. 119: 365-375, 2003. Go to original source...
    27. Guidi L., Degl'Innocenti E.: Ozone effects on high light-induced photoinhibition in Phaseolus vulgaris. - Plant Sci. 174: 590-596, 2008. Go to original source...
    28. Guo, X.R., Cao, K.F., Xu, Z.F.: Acclimation to irradiance in seedlings of three tropical rain forest Garcinia species after simulated gap formation. - Photosynthetica 44: 193-201, 2006. Go to original source...
    29. Heddad, M., Adamska, I.: Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family. - Proc. Natl. Acad. Sci. U.S.A. 97: 3741-3746, 2000. Go to original source...
    30. Hendrickson, L., Furbank, R.T., Chow, W.S.: A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. - Photosynth. Res. 82: 73-81, 2004. Go to original source...
    31. Hodges, D.M., Delong, J.M., Forney, C., Prange, R.K.: Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. - Planta 207: 604-611, 1999. Go to original source...
    32. Hogewoning, S.W., Harbinson, J.: Insights on the development, kinetics, and variation of photoinhibition using chlorophyll fluorescence imaging of a chilled, variegated leaf. - J. Exp. Bot. 58: 453-463, 2007.
    33. Horton, P., Wentworth, M., Ruban, A.: Control of the light harvesting function of chloroplast membranes: the LHCII-agreegation model for non-photochemical quenching. - FEBS Lett. 579: 4201-4206, 2005. Go to original source...
    34. Houter, N.C., Pons, T.L.: Gap size effects on photoinhibition in understorey saplings in tropical rainforest. - Plant Ecol. 179: 43-51, 2005. Go to original source...
    35. Johnson, G.N., Scholes, J.D., Horton, P., Young, A.J.: Relationship between carotenoid composition and growth habit in British plant species. - Plant Cell Environ. 16: 681-686, 1993. Go to original source...
    36. Kageyama, P.Y., Gandara, F.B.: [Grounds for rehabilitation of ecosystems forest.] - In: Galvão A.P.M., Porfírio-da-Silva V. (ed.): Forest Restoration - Fundamentals and Case Studies. Pp. 47-58. Embrapa, Colombo-PR 2005. [In Portuguese.]
    37. Kitajima, M., Butler, W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. - Biochim. Biophys. Acta 376: 105-115, 1975. Go to original source...
    38. Kitao, M., Yoneda, R., Tobita, H., Matsumoto, Y., Maruyama, Y., Arifin, A., Azani, M., Muhamad, M.N.: Susceptibility to photoinhibition in seedlings of six tropical fruit tree species native to Malaysia following transplantation to a degraded land. - Trees 20: 601-610, 2006. Go to original source...
    39. Klughammer, C., Schreiber, U.: Complementary FSII quantum yield calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method. - PAM Application Notes 1: 27-35, 2008.
    40. Kramer, D.M., Johnson, G., Kiirats, O., Edwards, G.E.: New fluorescence parameters for the determination of QA redox state and excitation energy fluxes. - Photosynth. Res. 79: 209-218, 2004. Go to original source...
    41. Leitsch, J., Schnettger, B., Critchley, C., Krause, G.H.: Two mechanisms of recovery from photoinhibition in vivo: reactivation of photosystem II related and unrelated to D1-protein turnover. - Planta 194: 15-21, 1994. Go to original source...
    42. Lichtenthaler, H.K., Langsdorf, G., Lenk, S., Buschmann, C.: Chlorophyll fluorescence imaging of photosynthetic activity with the flash-lamp fluorescence imaging system. - Photosynthetica 43: 355-369, 2005. Go to original source...
    43. Lichtenthaler, H.K., Ac, A., Marek, M.V., Kalina, J., Urban, O.: Differences in pigment composition, photosynthetic rates and chlorophyll fluorescence images of sun and shade leaves of four tree species. - Plant Physiol. Biochem. 45: 577-588, 2007a. Go to original source...
    44. Lichtenthaler, H.K., Babani, F., Langsdorf, G.: Chlorophyll fluorescence imaging of photosynthetic activity in sun and shade leaves of trees. - Photosynth. Res. 93: 235-244, 2007b. Go to original source...
    45. Lorenzi, H.: [Brazilian Trees: Manual of Identification and Growing of Native Tree Plants of Brazil.] - Instituto Plantarun de Estudos da Flora Ltda, Nova Odessa - São Paulo 2000. [In Portuguese.]
    46. Meng, Q., Siebke, K., Lippert, P., Baur, B., Mukherjee, U., Weis, E.: Sink-source transition in tobacco leaves visualized using chlorophyll fluorescence imaging. - New Phytol. 151: 585-595, 2001. Go to original source...
    47. Mittermeier, R.A., Myers, N., Gil, P.R., Mittermeier, C.G.: HOTSPOTS - Earth's biologically richest and most endangered terrestrial ecoregions. - Conservation International, the Mexican conservation organization Agrupación Sierra Madre and the cement company CEMEX, Mexico City 1999.
    48. Naidu, S.L., DeLucia, E.H.: Growth, allocation and water relations of shade-grown Quercus rubra L. saplings exposed to a late-season canopy gap. - Ann. Bot. 80: 335-344, 1997. Go to original source...
    49. Öquist, G., Anderson, J.M., McCaffery, S., Chow, W.S.: Mechanistic differences in photoinhibition of sun and shade plants. - Planta 188: 422-431, 1992a. Go to original source...
    50. Öquist, G., Chow, W.S., Anderson J.M.: Photoinhibition of photosynthesis represents a mechanism for the long-term regulation of photosystem II. - Planta 186: 450-460, 1992b. Go to original source...
    51. Oxborough, K.: Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthesis performance. - J. Exp. Bot. 55: 1195-1205, 2004. Go to original source...
    52. Poole, I., Weyers, J.D.B., Lawson, T., Raven, J.A.: Variations in stomatal density and index: implications for palaeoclimatic reconstructions. - Plant Cell Environ. 19: 705-712, 1996. Go to original source...
    53. Ramalho, J.C., Pons, T.L., Groeneveld, H.W., Nunes, M.A.: Photosynthetic responses of Coffea arabica leaves to a short-term high light exposure in relation to N availability. - Physiol. Plant 101: 229-239, 1997. Go to original source...
    54. Ribeiro, A.C.: [Recommendation for liming and fertility of soil for seedling, pits and flowerbeds. - In: Ribeiro A.C., Guimarães P.T.G., Álvares V.H. (ed.): Commissions of Soil Fertility of Minas Gerais State - Recommendations for the use of lime and fertilizers in Minas Gerais State.] - 5th Ed. Aproximação. Departamento de Solos da Universidade Federal de Viçosa, Viçosa-MG 1999. [In Portuguese.]
    55. Ribeiro, R.V., Souza, G.M., Oliveira, R.F., Machado, E.C.: Photosynthetic responses of tropical tree species from different successional groups under contrasting irradiance conditions. - Ver. Bras. Bot. 28: 149-161, 2005. Go to original source...
    56. Rozendaal, D.M.A., Hurtado, V.H., Pooter, L.: Plasticity in leaf traits of 38 tropical tree species in response to light: relationships with demand and adult stature. - Funct. Ecol. 20: 207-216, 2006. Go to original source...
    57. Ruban, A. V.: Plants in light. - Communicative & Integrative Biology 2: 1, 2009. Go to original source...
    58. Souza, G.M., Ribeiro, R.V., Prado, C.H.B.A., Damineli, D.S.C., Sato, A.M., Oliveira, M.S.: Using network connectance and autonomy analyses to uncover patterns of photosynthetic responses in tropical woody species. - Ecol. Complexity 6: 15-26, 2009. Go to original source...
    59. Takahashi, S., Badger, M. R.: Photoprotection in plants: a new light on photosystem II damage. - Trends Plant Sci. 16: 1-1, 2011. Go to original source...
    60. Takahashi, S., Murata, N.: How do environmental stresses accelerate photoinhibition? - Trends Plant Sci. 13: 178-182, 2008. Go to original source...
    61. Terashima, I.: Anatomy of non-uniform leaf photosynthesis. - Photosynth. Res. 31: 195-212, 1992. Go to original source...
    62. Thiele, A., Schirwitz, K., Winter, K., Krause, G.H.: Increase xanthophylls cycle activity and reduced D1 protein inactivation related to photoinhibition in two plant systems acclimated to excess light. - J. Plant Sci. 155: 237-250, 1996. Go to original source...
    63. Tobita, H., Utsugi, H., Kitao, M., Kayama, M., Uemura, A., Kitaoka, S., Maruyama, Y.: Variation in photoinhibition among Sasa senanensis, Quercus mongolica, and Acer mono in understory of a deciduous broad-leaved Forest exposed to canopy gaps caused by typhoons. - Trees 24: 307-319, 2010. Go to original source...
    64. Valladares, F., Niinemets, U.: Shade tolerance, a key plant feature of complex nature and consequences. - Annu. Rev. Ecol. Evol. System. 39: 237-257, 2008. Go to original source...
    65. Walters, R.G.: Towards an understanding of photosynthetic acclimation. - J. Exp. Bot. 56: 435-447, 2005.

    Return to the content