Photosynthetica, 2003 (vol. 41), issue 2

Photosynthetica 2003, 41(2):311-314 | DOI: 10.1023/B:PHOT.0000011969.64026.3c

Dynamic Changes of Photosynthetic Pigments in Soybean Callus under High Irradiance

D.M. Pandey1, K. H. Kim2, U. D. Yeo1
1 Faculty of Biological Science, Chonbuk National University, Chonju, Republic of Korea
2 National Honam Agricultural Experiment Station, Rural Development Administration, Iksan, Republic of Korea

Dynamic changes of neoxanthin (NEO), violaxanthin (VIO), anteraxanthin (ANT), zeaxanthin (ZEA), chlorophyll (Chl) a, Chl b, α-carotene, β-carotene, and their behaviour under increasing duration of high irradiance (HI) were investigated in the soybean hypocotyl callus culture. The calli were induced on solid (1.1 % agar) MS medium (pH 5.8) supplemented with 4.52 μM 2,4-D, 2.32 μM kinetin, and 3 % sucrose. After 30 d of culture, the green calli were irradiated with "white light" (133W m-2) for 0, 3.5, and 24 h. HPLC profiles were separated on a C18 column. With increasing duration of HI, the content of total carotenoids (Cars) increased, but the ratio of Chl a+b/Cars decreased. With lengthening the duration of HI, there was induction of ZEA. Contents of ANT, α-carotene, and β-carotene remained nearly constant, but ratio of ZEA/Chl a+b increased with lengthening the HI duration.

Keywords: carotenoids; chlorophyll; Glycine max; HPLC; photoinhibition; spectrophotometry; violaxanthin; zeaxanthin

Published: June 1, 2003Show citation

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Pandey, D.M., Kim, K.H., & Yeo, U.D. (2003). Dynamic Changes of Photosynthetic Pigments in Soybean Callus under High Irradiance. Photosynthetica, 41(2), 311-314. doi: 10.1023/B:PHOT.0000011969.64026.3c.
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    References

    1. Cunningham, F.X., Gantt, E.: Genes and enzymes of carotenoid biosynthesis in plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 557-583, 1998. Go to original source...
    2. de las Rivas, J., Abadía, A., Abadía, J.: A new reversed phase-HPLC method resolving all major higher plant photosynthetic pigments. - Plant Physiol. 91: 190-192, 1989. Go to original source...
    3. Demmig, B., Winter, K., Krüger, A., Czygan, F.-C.: Photoinhibition and zeaxanthin formation in intact leaves. A possible role of the xanthophyll cycle in the dissipation of excess light energy. - Plant Physiol. 84: 218-224, 1987. Go to original source...
    4. Demmig, B., Winter, K., Krüger, A., Czygan, F.-C.: Zeaxanthin and the heat dissipation of excess light energy in Nerium oleander exposed to a combination of high light and water stress. - Plant Physiol. 87: 17-24, 1988. Go to original source...
    5. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress. - Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    6. Demmig-Adams, B., Gilmore, A.M., Adams, W.W., III: In vivo functions of carotenoids in higher plants. - FASEB J. 10: 403-412, 1996. Go to original source...
    7. Koroleva, O.Y., Krause, G.H., Bruggemann, W.: Effects of long-term chilling under excessive light on xanthophyll cycle activity and non-photochemical fluorescence quenching in Lycopersicon genotypes. - J. Plant Physiol. 156: 341-349, 2000. Go to original source...
    8. Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature. - Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994. Go to original source...
    9. Minguez-Mosquera, M.I., Hornero-Méndez, D.: Separation and quantification of the carotenoid pigments in red peppers (Capsicum annum L.), paprika and oleoresin by reversedphase HPLC. - J. agr. Food Chem. 41: 1616-1620, 1993. Go to original source...
    10. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture. - Plant Physiol. 15: 473-493, 1962. Go to original source...
    11. Niyogi, K.K.: Photoprotection revisited: Genetic and molecular approaches. - Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 333-359, 1999. Go to original source...
    12. Osmond, C.B.: What is photoinhibition? Some insights from comparisons of shade and sun plants. - In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Field. Pp. 1-24. BIOS Scientific Publishers, Oxford 1994.
    13. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light. - Annu. Rev. Plant Physiol. 35:15-44, 1984. Go to original source...
    14. Ramalho, J.C., Pons, T.L., Groeneveld, H.W., Azinheira, H.G., Nunes, M.A.: Photosynthetic acclimation to high light conditions in mature leaves of Coffea arabica L.: role of xanthophylls, quenching mechanisms and nitrogen nutrition. - Aust. J. Plant Physiol. 27: 43-51, 2000.
    15. Semorádová, Š., Synková, H., Pospíšilová, J.: Responses of tobacco plantlets to changes of irradiance during transfer from in vitro to ex vitro conditions. - Photosynthetica 40: 605-614, 2002. Go to original source...
    16. Siefermann-Harms, D.: Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light harvesting function. - Biochim. biophys. Acta 811: 325-355, 1985. Go to original source...
    17. Val, J., Abadia, J., Heras, L., Monge, E.: Higher plant photosynthetic pigment analysis. Determination of carotenoids and chlorophylls by HPLC. - J. Micronutr. Anal. 2: 305-312, 1986.
    18. Wellburn, A.R.: The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. - J. Plant Physiol. 144: 307-313, 1994. Go to original source...

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