Photosynthetica, 2010 (vol. 48), issue 4

Photosynthetica 2010, 48(4):630-634 | DOI: 10.1007/s11099-010-0081-9

Physiological response of non-Bt and Bt cotton to short-term drought stress

P. Parimala1, K. Muthuchelian1,*
1 Department of Bioenergy, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, India

Drought stress triggered the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) both in non-Bt and Bt cotton with simultaneous production of antioxidant enzymes. And there was no significant difference between non-Bt and Bt cotton under drought stress. In contrast to this, we observed a significant reduction of Bt toxin proteins under 72 h of drought stress in Bt cotton.

Keywords: antioxidant enzymes; hydrogen peroxide; insecticidal Bt toxin proteins; ion leakage; malondialdehyde

Received: March 19, 2010; Accepted: October 15, 2010; Published: December 1, 2010Show citation

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Parimala, P., & Muthuchelian, K. (2010). Physiological response of non-Bt and Bt cotton to short-term drought stress. Photosynthetica48(4), 630-634. doi: 10.1007/s11099-010-0081-9.
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References

  1. Arora, R., Wisniewski, M.E., Scorza, R.: Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica L. Batsch). I. Seasonal changes in cold hardiness and polypeptides of bark and xylem tissues. - Plant Physiol. 99: 1562-1568, 1992. Go to original source...
  2. Asada, K.: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 601-639, 1999. Go to original source...
  3. Bartels, D., Sunkar, R.: Drought and salt tolerance in plants. - Crit. Rev. Plant Sci. 24: 23-58, 2005. Go to original source...
  4. Bates, L.S., Waldem, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
  5. Beauchamp, C., Fridovich, I.: Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. - Anal. Biochem. Rev. 44: 276-287, 1971. Go to original source...
  6. Blokhina, O., Virolainen, E., Fagerstedt, K.V.: Antioxidants, oxidative damage and oxygen deprivation stress: a review. - Ann. Bot. 91: 179-194, 2003. Go to original source...
  7. Bowler, C., Van Montagu, M., Inze, D.: Superoxide dismutases and stress tolerance. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 43: 83-116, 1992. Go to original source...
  8. Boyer, J.S.: Plant productivity and environment. - Science 218: 443-448, 1982. Go to original source...
  9. Bray, E.A. Bailey-Serres, J. Weretilnyk, E.: Responses to abiotic stress. - In: Buchanan, B.B., Gruissem, W. Jones, R.L. (ed.): Biochemistry and Molecular Biology of Plants. Pp. 1158-1203. ASPB, Waldorf 2000.
  10. Carlberg, I., Mannervik, B.: Glutathione reductase. - Methods Enzymol. 113: 484-490, 1985. Go to original source...
  11. Chen, D.H., Ye, G.Y., Yang, C.Q., Chen, Y., Wu, Y.K.: The effect of high temperature on the insecticidal properties of Bt Cotton. - Environ. Exp. Bot. 53: 333-340, 2005. Go to original source...
  12. Coviella, C.E., Stipanovic, R.D., Trumble, J.T.: Plant allocation to defensive compounds: interactions between elevated CO2 and nitrogen in transgenic cotton plants. - J. Exp. Bot. 53: 323-331, 2002. Go to original source...
  13. Davenport, S.B., Gallego, S.M., Benavides, M.P., Tomaro, M.L.: Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. cells. - Plant Growth Regul. 40: 81-88, 2003. Go to original source...
  14. Duan, B.L., Lu, Y.W., Yin, C.Y., Junttila, O., Li, C.Y.: Physiological responses to drought and shade in two contrasting Picea asperata populations. - Physiol. Plant. 124: 476-484, 2005. Go to original source...
  15. Gamble, P.E., Burke, J.J.: Effect of water stress on the chloroplast antioxidant system. I. Alterations in glutathione reductase activity. - Plant Physiol. 76: 615-621, 1984. Go to original source...
  16. Khan, N.A., Nazar, R., Anjum, N.A.: Growth, photosynthesis and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in ATP-sulfurylase activity under salinity stress. - Sci. Hort. 122: 455-460, 2009. Go to original source...
  17. Kramer, P.J., Boyer, J.S.: Water Relations of Plants and Soils. - Academic Press, San Diego 1995. Go to original source...
  18. Kumar, K.B., Khan, P.A.: Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. - Indian J. Exp. Biol. 20: 412-416, 1982.
  19. Lissner, J., Schierup, H.-H., Comn, F.A., Astorga, V.: Effect of climate on the salt tolerance of two Phragmites australis populations. I. Growth, inorganic solutes, nitrogen relations and osmoregulation. - Aquat. Bot. 64: 317-333, 1999. Go to original source...
  20. Loggini, B., Scartazza, A., Brugnoli, E., Navari-Izzo, F.: Antioxidative defense system, pigment composition and photosynthetic efficiency in two wheat cultivars subjected to drought. - Plant Physiol. 119: 1091-1099, 1999. Go to original source...
  21. Lowry, O.H., Rosebrough, N.J., Farn, A.L., Randall, R.J.: Protein measurement with the Folin-phenol reagent. - J. Biol. Chem. 193: 265-275, 1951.
  22. Lutts, S., Kinet, J.M., Bouharmont, J.: NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. - Ann. Bot. 78: 389-398, 1996. Go to original source...
  23. Noctor, G., Foyer, C.H.: Ascorbate and glutathione: Keeping active oxygen under control. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 249-279, 1998. Go to original source...
  24. Omaye, S.T., Turnbull, J.D., Sauberilich, H.E.: Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. - Meth. Enzymol. 62: 3-11, 1979. Go to original source...
  25. Rausch, T., Gromes, R., Liedschulte, V., Muller, I., Bogs, J., Galovic, V., Wachter, A.: Novel insight into the regulation of GSH biosynthesis in higher plants. - Plant Biol. 9: 565-572, 2007. Go to original source...
  26. Sheen, S.J., Calvert, J.: Studies on polyphenol content activities and isozymes of polyphenol oxidase and peroxidase during air-curing in three tobacco types. - Plant Physiol. 44: 199-204, 1969. Go to original source...
  27. Slavik, B.: Methods of Studying Plant Water Relations. - Academia, Prague 1974. Go to original source...
  28. Smirnoff, N.: The role of active oxygen in the response of plants to water deficit and desiccation. - New Phytol. 125: 27-58, 1993. Go to original source...
  29. Sofo, A., Dichio, B., Xiloyannis, C., Masia, A.: Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. - Physiol. Plant. 121: 58-65, 2004. Go to original source...
  30. Thomas, C.E., Mc Lean, L.R., Parkar, R.A., Ohleweiler, D.F.: Ascorbate and phenolic antioxidant interactions in prevention of liposomal oxidation. - Lipids 27: 543-550, 1992. Go to original source...
  31. Velikova, V., Yordanov, I., Edreva, A.: Oxidative stress and some antioxidant systems in acid rain treated bean plants: Protective role of exogenous polyamines. - Plant Sci. 151: 59-66, 2000. Go to original source...
  32. Verslues, P.E., Agarwal, M., Katiyar-Agarwal, S., Zhu, J.H., Zhu, J.K.: Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. - Plant J. 45: 523-539, 2006. Go to original source...
  33. Willekens, H., Chamnongpol, S., Davey, M., Schraudner, M., Langebartels, C., VanMontagu, M., Inze, D., VanCamp, W.: Catalase is a sink for H2O2 and is indispensable for stress defense in C3 plants. - EMBO J. 16: 4806-4816, 1997. Go to original source...
  34. Yin, C.Y., Peng, Y.H., Zang, R.G., Zhu, Y.P., Li, C.Y.: Adaptive responses of Populus kangdingensis to drought stress. - Physiol. Plant. 123: 445-451, 2005. Go to original source...