Photosynthetica, 2007 (vol. 45), issue 4

Photosynthetica 2007, 45(4):555-561 | DOI: 10.1007/s11099-007-0095-0

Changes in effects of ozone exposure on growth, photosynthesis, and respiration of Ginkgo biloba in Shenyang urban area

X. Y. He1, S. L. Fu1,2,*, W. Chen1, T. H. Zhao3, S. Xu1, Z. Tuba4
1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, P.R. China
2 School of Architecture and Urban Planning, Shenyang Jianzhu University, Shenyang, P.R. China
3 Department of Ecology, Shenyang Agricultural University, Shenyang, P.R. China
4 Department of Botany and Plant Physiology, Agricultural University of Gödöllő, Gödöllő, Hungary

An open-top chamber experiment was carried out from April through October 2006 to examine the effects of elevated (80 nmol mol-1) atmospheric O3 on Ginkgo biloba (4-years-old) in urban area. The air with ambient O3 (AA, ≈ 45 nmol mol-1) was used as control. The leaf mass and size, leaf area index, net photosynthetic rate (P N), apparent quantum yield, transpiration rate, and stomatal conductance were decreased by elevated O3 (EO) exposure. Visible foliar injury, which is light-brown flecks, was observed in the EO OTCs after 90 d of exposure. Carboxylation efficiency (ΦCO2) and photorespiration and dark respiration rates were enhanced by EO exposure in the first half of the season, but all of them turned to be lower than those of the AA control at the end of experiment. Stomata limitation of photosynthesis was significantly higher than control in the whole season (p<0.05). Chlorophyll (Chl) content was lower in EO variant than in the control and the difference became more and more apparent through the season. Hence the decrease in P N of G. biloba exposed to EO was the result of both stomatal and non-stomatal limitations. In the early season, the inhibition of photosynthesis was mainly caused by the stomatal limitation, and the earliest response was photoprotective down-regulation of photosynthesis but not photodamage. However, at the end of the season, the non-stomatal limiting factors such as decrease in Chl content, decrease in ΦCO2, and anti-oxidative enzyme activity became more important.

Keywords: carboxylation efficiency; chlorophyll; dark respiration; gas exchange; intercellular CO2 concentration; leaf area index; non-stomatal limitations; photorespiration; stomatal conductance; transpiration rate

Received: January 15, 2007; Accepted: March 26, 2007; Published: December 1, 2007Show citation

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He, X.Y., Fu, S.L., Chen, W., Zhao, T.H., Xu, S., & Tuba, Z. (2007). Changes in effects of ozone exposure on growth, photosynthesis, and respiration of Ginkgo biloba in Shenyang urban area. Photosynthetica, 45(4), 555-561. doi: 10.1007/s11099-007-0095-0.
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    References

    1. Bassin, S., Kölliker, R., Creton, C., Bertossa, M., Widmer, F., Bungener, P., Fuhrer, J.: Intra-specific variability of ozone sensitivity in Centaurea jacea L., a potential bioindicator for elevated ozone concentrations.-Environ. Pollut. 131: 1-12, 2004. Go to original source...
    2. Berry, J., Björkman, O.: Photosynthetic response and adaptation to temperature in higher plants.-Annu. Rev. Plant Physiol. 31: 491-543, 1980. Go to original source...
    3. Bridi, R., Crossetti, F.P., Steffen, V.M., Henriques, A.T.: The antioxidant activity of standardized extract of Ginkgo biloba (EGb761) in rats.-Phytother. Res. 15: 449-451, 2001. Go to original source...
    4. Bungener, P., Balls, G.R., Nussbaum, S., Geissmann, M., Grub, A., Fuhrer, J.: Leaf injury characteristics of grassland species exposed to ozone in relation to soil moisture condition and vapour pressure deficit.-New Phytol. 142: 271-282, 1999. Go to original source...
    5. Cai, S.-Q., Xu, D.-Q.: Relationship between the CO2 compensation point and photorespiration in soybean leaves.-Acta phytophysiol. sin. 26: 545-550, 2000.
    6. Calatayud, A., Iglesias, D.J., Talón, M., Barreno, E.: Effects of 2-month ozone exposure in spinach leaves on photosynthesis, antioxidant systems and lipid peroxidation.-Plant Physiol. Biochem. 41: 839-845, 2003. Go to original source...
    7. Calatayud, A., Iglesias, D.J., Talón, M., Barreno, E.: Effects of long-term ozone exposure on citrus: Chlorophyll a fluorescence and gas exchange.-Photosynthetica 44: 548-554, 2006. Go to original source...
    8. Ciompi, S., Castagna, A., Ranieri, A., Nali, C., Lorenzini, G., Soldatini, G.F.: CO2 assimilation, xanthophyll cycle pigments and PSII efficiency in pumpkin plants as affected by ozone fumigation.-Physiol. Plant. 101: 881-889, 1997. Go to original source...
    9. Degl'Innocenti, E., Guidi, L., Soldatini, G.F.: Characterisation of the photosynthetic response of tobacco leaves to ozone: CO2 assimilation and chlorophyll fluorescence.-J. Plant Physiol. 159: 845-853, 2002. Go to original source...
    10. Ding, G.-A., Xu, X.-B., Tang, J.: Surface ozone characteristics at three stations in China.-Acta meteorol. sin. 15: 21-28, 2001.
    11. Donnelly, A., Craigon, J., Black, C.R., Colls, J.J., Landon, G.: Does elevated CO2 ameliorate the impact of O3 on chlorophyll content and photosynthesis in potato (Solanum tuberosum)?-Physiol. Plant. 111: 501-511, 2001.
    12. Farage, P.K., Long, S.P.: The effects of O3 fumigation during leaf development on photosynthesis of wheat and pea: An in vivo analysis.-Photosynth. Res. 59: 1-7, 1999. Go to original source...
    13. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    14. Fuhrer, J., Booker, F.: Ecological issues related to ozone: agricultural issues.-Environ. int. 29: 141-154, 2003. Go to original source...
    15. Gail, B.M.: Ginkgo biloba: a review of quality, safety, and efficacy.-Nutr. Clin. Care 4: 140-147, 2001.
    16. Graham, N., Ana, C.M., Arisi, L.J., Christine, H.F.: Photorespiratory glycine enhances glutathione accumulation in both the chloroplastic and cytosolic compartments.-J. exp. Bot. 50: 1157-1167, 1999.
    17. Grandjean Grimm, A., Fuhrer, J.: The response of spring wheat (Triticum aestivum L.) to ozone at higher elevations. III. Responses of leaf and canopy gas exchange, and chlorophyll fluorescence to ozone flux.-New Phytol. 122: 321-328, 1992. Go to original source...
    18. Guidi, L., Nali, C., Lorenzini, G., Filippi, F., Soldatini, G.F.: Effect of chronic ozone fumigation on the photosynthetic process of poplar clones showing different sensitivity.-Environ. Pollut. 113: 245-254, 2001. Go to original source...
    19. Guo, J.-P., Wang, C.-Y., Wen, M., Bai, Y.-M., Huo, Z.-G.: The experimental study on the impact of atmospheric O3 variation on rice.-Acta agron. sin. 27: 822-826, 2001.
    20. He, X.-Y., Ruan, Y.-N., Chen, W., Lu, T.: Responses of the anti-oxidative system in leaves of Gingo biloba to elevated ozone concentration in an urban area.-Bot. Stud. 47: 409-416, 2006.
    21. Heath, R.L.: Possible mechanisms for the inhibition of photosynthesis by ozone.-Photosynth. Res. 39: 439-451, 1994. Go to original source...
    22. Jin, M.-H., Feng, Z.-W., Zhang, F.-Z.: Effects of ozone on membrane lipid peroxidation and antioxidant system of rice leaves.-Environ. Sci. 21: 1-5, 2000.
    23. Keutgen, A.J., Noga, G., Pawelzik, E.: Cultivar-specific impairment of strawberry growth, photosynthesis, carbohydrate and nitrogen accumulation by ozone.-Environ. exp. Bot. 53: 271-280, 2005. Go to original source...
    24. Legge, A.H., Grünhage, L., Nosal, M., Jäger, H.J., Krupa, S.V.: Ambient ozone and adverse crop response: an evaluation of North American and European data as they relate to exposure indices and critical levels.-Angew. Bot. 69: 192-205, 1995.
    25. Lütz, C., Anegg, S., Gerant, D., Alaouni-Sossé, B., Gérard, J., Dizengremel, P.: Beech trees exposed to high CO2 and to simulated summer ozone levels: Effects on photosynthesis, chloroplast components and leaf enzyme activity.-Physiol. Plant. 109: 252-259, 2000. Go to original source...
    26. Major, R.T.: The ginkgo, the most ancient living tree: The resistance of Ginkgo biloba L. to pests accounts in part for the longevity of this species.-Science 157: 1270-1273, 1967. Go to original source...
    27. Manninen, S., Siivonen, N., Timonen, U., Huttunen, S.: Differences in ozone response between two Finnish wild strawberry populations.-Environ. exp. Bot. 49: 29-39, 2003. Go to original source...
    28. McPherson, E.G., Simpson, J.R.: Air pollutant uptake by Sacramento's urban forest.-J. Arboricult. 24: 224-234, 1998.
    29. Mikkelsen, T.N., Dodell, B., Lütz, C.: Changes in pigment concentration and composition in Norway spruce induced by long-term exposure to low levels of ozone.-Environ. Pollut. 87: 197-205, 1995. Go to original source...
    30. Moldau, H., Sober, J., Sober, A.: Impact of acute ozone exposure on CO2 uptake by two cultivars of Phaseolus vulgaris L.-Photosynthetica 28: 133-141, 1993.
    31. Muraoka, H., Tang, Y., Terashima, I., Koizumi, H., Washitani, I.: Contribution of diffusional limitation, photoinhibition and photorespiration to midday depression of photosynthesis in Arisaema heterophyllum in the natural high light.-Plant Cell Environ. 23: 235-250, 2000. Go to original source...
    32. Musselman, R.C., Massman, W.J.: Ozone flux to vegetation and its relationship to plant response and ambient air quality standards.-Atmos. Environ. 33: 65-73, 1999.
    33. Ollerenshaw, J.H., Lyons, T., Barnes, J.D.: Impact of ozone on the growth and yield of field-grown winter oilseed rape.-Environ. Pollution 104: 53-59, 1999. Go to original source...
    34. Paul, E.G., Larry, C., Gary, L.W.: Ginkgo biloba: a cognitive enhancer?-Psychol. Sci. public Interest 3: 2-11, 2002
    35. Pell, E.J., Eckardt, N., Enyedi, A.J.: Timing of ozone stress and resulting status of ribulose bisphosphatase carboxylase/oxygenase and associated net photosynthesis.-New Phytol. 120: 397-405, 1992. Go to original source...
    36. Pell, E.J., Eckardt, N.A., Glick, R.E.: Biochemical and molecular basis for impairment of photosynthetic potential.-Photosynth. Res. 39: 453-462, 1994. Go to original source...
    37. Pell, E.J., Sinn, J.P., Brendley, B.W., Samuelson, L., Vinten-Johansen, C., Tien, M., Skillman, J.: Differential response of four tree species to ozone-induced acceleration of foliar senescence.-Plant Cell Environ. 22: 779-790, 1999. Go to original source...
    38. Plażek, A., Rapacz, M., Skoczowski, A.: Effects of ozone fumigation on photosynthesis and membrane permeability in leaves of spring barley, meadow fescue, and winter rape.-Photosynthetica 38: 409-413, 2000.
    39. Reich, P.B.: Quantifying plant response to ozone: a unifying theory.-Tree Physiol. 3: 63-91, 1987. Go to original source...
    40. Reichenauer, T., Bolhàr-Nordenkampf, H.R., Ehrlich, U., Soja, G., Postl, W.F., Halbwachs, F.: The influence of ambient and elevated ozone concentrations on photosynthesis in Populus nigra.-Plant Cell Environ. 20: 1061-1069, 1997. Go to original source...
    41. Vandermeiren, K., Black, C., Pleijel, H., De Temmerman, L.: Impact of rising tropospheric ozone on potato: effects on photosynthesis, growth, productivity and yield quality.-Plant Cell Environ. 28: 982-996, 2005. Go to original source...
    42. Volin, J.C., Reich, P.B., Givnish, T.J.: Elevated carbon dioxide ameliorates the effects of ozone on photosynthesis and growth: species respond similarly regardless of photosynthetic pathway or plant functional group.-New Phytol. 138: 315-325, 1998. Go to original source...
    43. Vu, J.C.V.: Acclimation of peanut (Arachis hypogaea L.) leaf photosynthesis to elevate growth CO2 and temperature.-Environ. exp. Bot. 53: 85-95, 2005. Go to original source...
    44. Walker, D.A.: Automated measurement of leaf photosynthetic O2 evolution as a function of photon flux density.-Phil. Trans. roy. Soc. London B 323: 313-326, 1989. Go to original source...
    45. Wellburn, A.R.: The spectral determination of chlorophylls a and b as well as total carotenoids, using various solvents with spectrophotometers of different resolution.-J. Plant Physiol. 144: 307-313, 1994. Go to original source...
    46. Woo, S.Y., Hinckley, T.M.: The effects of ozone on growth and stomatal response in the F2 generation of hybrid poplar (Populus trichocarpa×Populus deltoides).-Biol. Plant. 49: 395-404, 2005. Go to original source...

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