Photosynthetica, 2015 (vol. 53), issue 3

Photosynthetica 2015, 53(3):471-477 | DOI: 10.1007/s11099-015-0116-3

Differential blockage of photosynthetic electron flow in young and mature leaves of Arabidopsis thaliana by exogenous proline

I. Sperdouli1, M. Moustakas1,*
1 Department of Botany, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece

Responses of the photosynthetic electron transport system of chloroplasts to exogenous proline application were evaluated in young and mature leaves of Arabidopsis thaliana plants under optimal growth conditions. Exogenous proline application (10 mM) during the 4th week of growth increased proline accumulation in young leaves more than in mature leaves, and possibly due to its degradation producing NADPH, decreased significantly the ratio of NADP+/NADPH in both leaf types compared with controls (without proline). However, the ratio of NADP+/NADPH remained significantly higher in the young leaves, suggesting lower proline degradation which resulted in less reduced plastoquinone pool than that in the mature leaves, under both low light [130 μmol(photon) m-2 s-1] and high light [1,200 μmol(photon) m-2 s-1] treatments. The young leaves seemed to adjust nonphotochemical fluorescence quenching in order to maintain a better PSII quantum yield. We concluded that under optimal growth conditions exogenous proline results in overreduction of the plastoquinone pool and blockage of photosynthetic electron flow due to accumulation of NADPH. We suggest that optimum concentrations of proline are required for optimal PSII photochemistry.

Keywords: chlorophyll fluorescence; electron transport rate; leaf age; reactive oxygen species

Received: June 8, 2014; Accepted: October 23, 2014; Published: September 1, 2015Show citation

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Sperdouli, I., & Moustakas, M. (2015). Differential blockage of photosynthetic electron flow in young and mature leaves of Arabidopsis thaliana by exogenous proline. Photosynthetica, 53(3), 471-477. doi: 10.1007/s11099-015-0116-3.
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