Photosynthetica 2017, 55(2):317-328 | DOI: 10.1007/s11099-016-0241-7

Photosynthetic responses of a wheat (Asakaze)-barley (Manas) 7H addition line to salt stress

D. Szopkó¹, É. Darkó², I. Molnár², K. Kruppa², B. Háló¹, A. Vojtkó³, M. Molnár-Láng², S. Dulai^1,*

¹ Department of Plant Physiology, Faculty of Science, Eszterházy University, Eger, Hungary

² Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary

³ Department of Botany, Faculty of Science, Eszterházy University, Eger, Hungary

The photosynthetic responses to salt stress were examined in a wheat (Triticum aestivum L. cv. Asakaze)-barley (Hordeum vulgare L. cv. Manas) 7H addition line having elevated salt tolerance and compared to the parental wheat genotype. For this purpose, increasing NaCl concentrations up to 300 mM were applied and followed by a 7-day recovery period. Up to moderate salt stress (200 mM NaCl), forcible stomatal closure, parallel with a reduction in the net assimilation rate (P _N), was only observed in wheat, but not in the 7H addition line or barley. Since the photosynthetic electron transport processes of wheat were not affected by NaCl, the impairment in P _N could largely be accounted for the salt-induced decline in stomatal conductance (g _s), accompanied by depressed intercellular CO₂ concentration and carboxylation efficiency. Both, P _N and nonstomatal limitation factors (L_ns) were practically unaffected by moderate salt stress in barley and in the 7H addition line due to the sustained g _s, which might be an efficient strategy to maintain the efficient photosynthetic activity and biomass production. At 300 mM NaCl, both P _N and g _s decreased significantly in all the genotypes, but the changes in P _N and L_ns in the 7H addition line were more favourable similar to those in wheat. The downregulation of photosynthetic electron transport processes around PSII, accompanied by increases in the quantum yield of regulated energy dissipation and of the donor side limitation of PSI without damage to PSII, was observed in the addition line and barley during severe stress. Incomplete recovery of P _N was observed in the 7H addition line as a result of declined PSII activity probably caused by enhanced cyclic electron flow around PSI. These results suggest that the better photosynthetic tolerance to moderate salt stress of barley can be manifested in the 7H addition line which may be a suitable candidate for improving salt tolerance of wheat.

Keywords: chlorophyll fluorescence induction; leaf gas exchange; recovery; salt tolerance; wheat-barley addition

Received: January 25, 2016; Accepted: May 5, 2016; Published: June 1, 2017Show citation