Photosynthetica, 2004 (vol. 42), issue 2

Photosynthetica 2004, 42(2):201-212 | DOI: 10.1023/B:PHOT.0000040591.74037.f1

Influence of Temperature on the Effects of Artificially Enhanced UV-B Radiation on Aquatic Bryophytes Under Laboratory Conditions

E. Núñez-Olivera1, J. Martínez-Abaigar1, R. Tomás1, N. Beaucourt1, M. Arróniz-Crespo1
1 Universidad de La Rioja, Complejo Científico-Tecnológico, Logroño (La Rioja), Spain

We examined, under laboratory conditions, the influence of temperature (2 °C vs. 10 °C) on the physiological responses of two aquatic bryophytes from a mountain stream to artificially enhanced UV-B radiation for 82 d. These organisms may be exposed naturally to relatively low temperatures and high levels of UV-B radiation, and this combination is believed to increase the adverse effects of UV-B radiation. In the moss Fontinalis antipyretica, UV-B-treated samples showed severe physiological damages, including significant decreases in chlorophyll (Chl) and carotenoid (Car) contents, Chl a/b and Chl/phaeopigment ratios, Chl a fluorescence parameters Fv/Fm and ΦPS2, electron transport rate (ETRmax), and growth. In the liverwort Jungermannia cordifolia, UV-B radiation hardly caused any physiological change except for growth reduction. Thus, this liverwort seemed to be more tolerant to UV-B radiation than the moss under the specific experimental conditions used, maybe partly due to the accumulation of UV-B absorbing compounds. The influence of temperature on the effects of UV-B radiation depended on the species: the higher the UV-B tolerance, the lower the influence of temperature. Also, different physiological variables showed varied responses to this influence. Particularly, the lower temperature used in our study enhanced the adverse effects of UV-B radiation on important physiological variables such as Fv/Fm, growth, and Chl/phaeopigment ratios in the UV-B-sensitive F. antipyretica, but not in the more UV-B-tolerant J. cordifolia. Thus, the adverse effects of cold and UV-B radiation were apparently additive in the moss, but this additiveness was lacking in the liverwort. The Principal Components Analyses (PCA) conducted for both species with the physiological data obtained after 36 and 82 d of culture confirmed the above results. Under natural conditions, the relatively high water temperatures in summer might facilitate the acclimation of aquatic bryophytes from mountain streams to high levels of UV-B radiation. This may be relevant to predict the consequences of concomitant global warming and increasing UV-B radiation.

Keywords: carotenoids; chlorophylls; Fontinalis; Jungermannia; net photosynthetic rate; pheopigments; respiration

Published: June 1, 2004Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Núñez-Olivera, E., Martínez-Abaigar, J., Tomás, R., Beaucourt, N., & Arróniz-Crespo, M. (2004). Influence of Temperature on the Effects of Artificially Enhanced UV-B Radiation on Aquatic Bryophytes Under Laboratory Conditions. Photosynthetica, 42(2), 201-212. doi: 10.1023/B:PHOT.0000040591.74037.f1.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Altamirano, M., Flores-Moya, A., Figueroa, F.L.: Effects of UV radiation and temperature on growth of germlings of three species of Fucus (Phaeophyceae).-Aquat. Bot. 75: 9-20, 2003. Go to original source...
    2. Björn, L.O., Callaghan, T.V., Gehrke, C., Johanson, U., Sonesson, M., Gwynn-Jones, D.: The problem of ozone de-pletion in northern Europe.-Ambio 27: 275-279, 1998.
    3. Björn, L.O., Teramura, A.H.: Simulation of daylight ultraviolet radiation and effects of ozone depletion.-In: Young, A.R. (ed.): Environmental UV Photobiology. Pp. 41-71. Plenum Press, New York 1993. Go to original source...
    4. Bowden, W.B., Arscott, D., Pappathanasi, D., Finlay, J., Glime, J.M., LaCroix, J., Liao, C.L., Hershey, A., Lampella, T., Peterson, B., Wollheim, W., Slavik, K., Shelley, B., Chesterton, M.B., Lachance, J.A., LeBlanc, R.M., Steinman, A., Suren, A.: Roles of bryophytes in stream ecosystems.-J. north amer. benthol. Soc. 18: 151-184, 1999.
    5. Bradford, M.: A rapid and sensitive method for the quantifica-tion of microgram quantities of protein utilizing the principle of protein dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    6. Buffoni Hall, R.S., Paulsson, M., Duncan, K., Tobin, A.K., Widell, S., Bornman, J.F.: Water-and temperature-dependen-ce of DNA damage and repair in the fruticose lichen Cladonia arbuscula ssp. mitis exposed to UV-B radiation.-Physiol. Plant. 118: 371-379, 2003. Go to original source...
    7. Caldwell, M.M.: Solar UV irradiation and the growth and deve-lopment of higher plants.-In: Giese, A.C. (ed.): Photophy-siology. Vol. 6. Pp. 131-177. Academic Press, New York 1971. Go to original source...
    8. Csintalan, Z., Tuba, Z., Takács, Z., Laitat, E.: Responses of nine bryophyte and one lichen species from different microhabitats to elevated UV-B radiation.-Photosynthetica 39: 317-320, 2001. Go to original source...
    9. Day, T.A., Neale, P.J.: Effects of UV-B radiation on terrestrial and aquatic primary producers.-Annu. Rev. Ecol. Syst. 33: 371-396, 2002. Go to original source...
    10. Falkowski, P.G., Raven, J.A.: Aquatic Photosynthesis.-Blackwell Science, Oxford 1997.
    11. Franklin, L.A., Forster, R.M.: The changing irradiance environ-ment: consequences for marine macrophyte physiology, pro-ductivity and ecology.-Eur. J. Phycol. 32: 207-232, 1997. Go to original source...
    12. Gartia, S., Pradhan, M.K., Joshi, P.N., Biswall, U.C., Biswal, B.: UV-A irradiation guards the photosynthetic apparatus against UV-B-induced damage.-Photosynthetica 41: 545-549, 2003. Go to original source...
    13. Gehrke, C.: Effects of enhanced UV-B radiation on production related properties of a Sphagnum fuscum dominated subarctic bog.-Funct. Ecol. 12: 940-947, 1998. Go to original source...
    14. Gehrke, C.: Impacts of enhanced ultraviolet-B radiation on mosses in a subarctic heath ecosystem.-Ecology 80: 1844-1851, 1999. Go to original source...
    15. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron trans-port and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    16. Germ, M., Gaberš è ik, A.: Comparison of aerial and submerghed leaves in two amphibious species, Myosotis scorpioides and Ranunculus trichophyllus.-Photosynthetica 41: 91-96, 2003. Go to original source...
    17. Glime, J.M.: Phytogeographic implications of a Fontinalis (Fontinalaceae) growth model based on temperature and flow.E. conditions for six species.-Mem. New York bot. Garden 45: 154-170, 1987.
    18. Gómez, I., Figueroa, F.L., Sousa-Pinto, I., Viñegla, B., Pérez-Rodríguez, E., Maestre, C., Coelho, S., Felga, A., Pereira, R.: Effects of UV radiation and temperature on photosynthesis as measured by PAM fluorescence in the red alga Gelidium pulchellum (Turner) Kützing.-Bot. mar. 44: 9-16, 2001. Go to original source...
    19. Gwynn-Jones, D., Lee, J.A., Johanson, U., Phoenix, G.K., Callaghan, T.V., Sonesson, M.: The responses of plant func-tional Types to enhanced UV-B radiation.-In: Rozema, J. (ed.): Stratospheric Ozone Depletion: the Effects of Enhanced UV-B Radiation on Terrestrial Ecosystems. Pp. 173-185. Backhuys Publishers, Leiden 1999.
    20. Häder, D.P.: The Effects of Ozone Depletion on Aquatic Eco-systems.-Academic Press, London 1997.
    21. Hendry, G.A.F., Price, A.H.: Stress indicators: chlorophylls and carotenoids.-In: Hendry, G.A.F., Grime, J.P. (ed.): Methods in Comparative Ecology. A Laboratory Manual. Pp. 148-152. Chapman & Hall, London 1993. Go to original source...
    22. Ihle, C., Laasch, H.: Inhibition of photosystem II by UV-B radiation and the conditions for recovery in the liverwort Conocephalum conicum Dum.-Bot. Acta 109: 199-205, 1996. Go to original source...
    23. Jansen, M.A.K., Gaba, V., Greenberg, B.M.: Higher plants and UV-B radiation: balancing damage, repair and acclimation.-Trends Plant Sci. 3: 131-135, 1998. Go to original source...
    24. Lud, D., Moerdijk, T.C.W., Van de Poll, W.H., Buma, A.G.J., Huiskes, A.H.L.: DNA damage and photosynthesis in Antarctic and Arctic Sanionia uncinata (Hedw.) Loeske under ambient and enhanced levels of UV-B radiation.-Plant Cell Environ. 25: 1579-1589, 2002. Go to original source...
    25. Madronich, S., McKenzie, R.L., Björn, L.O., Caldwell, M.M.: Changes in biologically active ultraviolet radiation reaching the Earth's surface.-J. Photochem. Photobiol. B 46: 5-19, 1998. Go to original source...
    26. Mark, U., Tevini, M.: Effects of solar ultraviolet-B radiation, temperature and CO2 on growth and physiology of sunflower and maize seedlings.-Plant Ecol. 128: 224-234, 1997. Go to original source...
    27. Martínez-Abaigar, J., NÚñez-Olivera, E.: Ecophysiology of pho-tosynthetic pigments in aquatic bryophytes.-In: Bates, J.W., Ashton, N.W., Duckett, J.G. (ed.): Bryology for the Twenty-first Century. Pp. 277-292. Maney Publishing and the British Bryological Society, Leeds 1998. Go to original source...
    28. Martínez-Abaigar, J., NÚñez-Olivera, E., Beaucourt, N., García-Álvaro, M.A., Tomás, R., Arróniz, M.: Different physiologi-cal responses of two aquatic bryophytes to enhanced ultra-violet-B radiation.-J. Bryol. 25: 17-30, 2003. Go to original source...
    29. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence-a prac-tical guide.-J. exp. Bot. 51: 659-668, 2000. Go to original source...
    30. Mazza, C.A., Battista, D., Zima, A.M., Szwarcberg-Bracchitta, M., Giordano, C.V., Acevedo, A., Scopel, A.L., Ballaré, C.L.: The effects of solar ultraviolet-B radiation on the growth and yield of barley are accompanied by increased DNA damage and antioxidant responses.-Plant Cell Environ. 22: 61-70, 1999. Go to original source...
    31. Newsham, K.K., Hodgson, D.A., Murray, A.W.A., Peat, H.J., Lewis Smith, R.I.: Response of two Antarctic bryophytes to stratospheric ozone depletion.-Global Change Biol. 8: 972-983, 2002. Go to original source...
    32. Niemi, R., Martikainen, P.J., Silvola, J., Sonninen, E., Wulff, A., Holopainen, T.: Responses of two Sphagnum moss species and Eriophorum vaginatum to enhanced UV-B in a summer of low UV intensity.-New Phytol. 156: 509-515, 2002a. Go to original source...
    33. Niemi, R., Martikainen, P.J., Silvola, J., Wulff, A., Turtola, S., Holopainen, T.: Elevated UV-B radiation alters fluxes of methane and carbon dioxide in peatland microcosms.-Global Change Biol. 8: 361-371, 2002b. Go to original source...
    34. NÚñez-Olivera, E., García-Álvaro, A., Beaucourt, N., Martínez-Abaigar, J.: Changes in element concentrations in aquatic bryophytes over an annual cycle.-Arch. Hydrobiol. 152: 253-277, 2001.
    35. Rader, R.B., Belish, T.A.: Short-term effects of ambient and en-hanced UV-B on moss (Fontinalis neomexicana) in a moun-tain stream.-J. Freshwater Ecol. 12: 395-403, 1997. Go to original source...
    36. Rousseaux, M.C., Ballaré, C.L., Giordano, C.V., Scopel, A.L., Zima, A.M., Szwarcberg-Bracchitta, M., Searles, P.S., Caldwell, M.M., Díaz, S.B.: Ozone depletion and UVB radia-tion: impact on plant DNA damage in southern South America.-Proc. nat. Acad. Sci. USA 96: 15310-15315, 1999. Go to original source...
    37. Schnitzler, J.P., Jungblut, T.P., Heller, W., Köfferlein, M., Hutzler, P., Heinzmann, U., Schmelzer, E., Ernst, D., Langebartels, C., Sandermann, H.J.: Tissue localization of u.v.-B-screening pigments and of chalcone synthase mRNA in needles of Scots pine seedlings.-New Phytol. 132: 247-258, 1996. Go to original source...
    38. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluores-cence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In: Schulze, E.D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer, Berlin-Heidelberg-New York 1995. Go to original source...
    39. Searles, P.S., Flint, S.D., Caldwell, M.M.: A meta-analysis of plant field studies simulating stratospheric ozone depletion.-Oecologia 127: 1-10, 2001.
    40. Searles, P.S., Flint, S.D., Díaz, S.B., Rousseaux, M.C., Ballaré, C.L., Caldwell, M.M.: Solar ultraviolet-B radiation influence on Sphagnum bog and Carex fen ecosystems: first field season findings in Tierra del Fuego, Argentina.-Global Change Biol. 5: 225-234, 1999. Go to original source...
    41. Searles, P.S., Flint, S.D., Díaz, S.B., Rousseaux, M.C., Ballaré, C.L., Caldwell, M.M.: Plant response to solar ultraviolet-B ra-diation in a southern South American Sphagnum peatland.-J. Ecol. 90: 704-713, 2002. Go to original source...
    42. Šesták, Z.: Determination of chlorophylls a and b.-In: Šesták, Z., È atský, J., Jarvis, P.G. (ed.): Plant Photosynthetic Produc-tion. Manual of Methods. Pp. 672-701. Dr W. Junk N.V. Publishers, The Hague 1971.
    43. Sonesson, M., Callaghan, T.V., Carlsson, B.A.: Effects of en-hanced ultraviolet radiation and carbon dioxide concentration on the moss Hylocomium splendens.-Global Change Biol. 2: 67-73, 1996. Go to original source...
    44. Sonesson, M., Carlsson, B.A., Callaghan, T.V., Halling, S., Björn, L.O., Bertgren, M., Johanson, U.: Growth of two peat-forming mosses in subarctic mires: species interactions and effects of simulated climate change.-Oikos 99: 151-160, 2002. Go to original source...
    45. Šprtová, M., Špunda, V., Kalina, J., Marek, M.V.: Photosyn-thetic UV-B response of beech (Fagus sylvatica L.) saplings.-Photosynthetica 41: 533-543, 2003.
    46. Van De Poll, W.H., Bischof, K., Buma, A.G.J., Breeman, A.M.: Habitat related variation in UV tolerance of tropical marine red macrophytes is not temperature dependent.-Physiol. Plant. 118: 74-83, 2003. Go to original source...
    47. Van De Poll, W.H., Eggert, A., Buma, A.G.J., Breeman, A.M.: Temperature dependence of UV radiation effects in Arctic and temperate isolates of three red macrophytes.-Eur. J. Phycol. 37: 59-68, 2002. Go to original source...

    Return to the content