Photosynthetica, 2015 (vol. 53), issue 2

Photosynthetica 2015, 53(2):201-206 | DOI: 10.1007/s11099-015-0097-2

Extracellular ATP affects chlorophyll fluorescence of kidney bean (Phaseolus vulgaris) leaves through Ca2+ and H2O2-dependent mechanism

H. Q. Feng1,*, Q. S. Jiao1, K. Sun1, L. Y. Jia1, W. Y. Tian1
1 College of Life Science, Northwest Normal University, Lanzhou, China

Extracellular ATP (eATP) has been considered as an important extracellular compound to mediate several physiological processes in plant cells. We investigated the effects of eATP on chlorophyll (Chl) fluorescence characteristics of kidney bean (Phaseolus vulgaris) leaves. Treatment with exogenous ATP at 1 mM showed no significant effect on the maximal photochemical efficiency of PSII. However, the treatment significantly enhanced the values of the PSII operating efficiency (ΦPSII), rate of photosynthetic electron transport through PSII (ETR), and photochemical quenching (qP), while the values of the nonphotochemical quenching (qN) and quantum yield of regulated energy dissipation of PSII (YNPQ) significantly decreased. Our observations indicated that eATP stimulated the PSII photochemistry in kidney bean leaves. Similarly, the treatment with exogenous Ca2+ or H2O2 at 1 mM caused also the significant increase in ΦPSII, qP, and ETR and the significant decrease in qN and YNPQ. LaCl3 (an inhibitor of Ca2+ channels) and dimethylthiourea (a scavenger of H2O2) abolished the effects of exogenous ATP. The results suggest that the role of eATP in enhancing the PSII photochemistry could be related to a Ca2+ or H2O2 signaling pathway.

Keywords: photosynthesis; reactive oxygen species; signaling molecules

Received: September 22, 2014; Accepted: November 28, 2014; Published: June 1, 2015Show citation

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Feng, H.Q., Jiao, Q.S., Sun, K., Jia, L.Y., & Tian, W.Y. (2015). Extracellular ATP affects chlorophyll fluorescence of kidney bean (Phaseolus vulgaris) leaves through Ca2+ and H2O2-dependent mechanism. Photosynthetica, 53(2), 201-206. doi: 10.1007/s11099-015-0097-2.
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