Photosynthetica, 2003 (vol. 41), issue 2

Photosynthetica 2003, 41(2):177-184 | DOI: 10.1023/B:PHOT.0000011949.91626.ec

Different Mechanisms for Photosystem 2 Reversible Down-Regulation in Pumpkin and Soybean Leaves Under Saturating Irradiance

Hai-Bo Zhang1, Da-Quan Xu1
1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

After saturating irradiation for 3 h (SI), the original fluorescence F0 increased while the photosystem 2 (PS2) photochemical efficiency (Fv/Fm) declined significantly. These parameters could largely recover to the levels of dark-adapted leaves after 3 h of subsequent dark recovery. No net loss of the D1 proteins occurred after SI. Soybean and pumpkin leaves had different responses to SI. Low temperature fluorescence parameters, F685 and F685/F735, decreased significantly in soybean leaves but not in pumpkin leaves. Part of the light-harvesting complex LHC2 dissociated from PS2 complexes in soybean leaves but not in pumpkin leaves, as shown by sucrose density gradient centrifugation and SDS-PAGE. The photon-saturated PS2 electron transport activity declined significantly in pumpkin thylakoids but not in soybean thylakoids. In addition, a large amount of phosphorylated D1 proteins was found in dark-adapted soybean leaves but not in dark-adapted pumpkin leaves. Hence at excessive irradiance soybean and pumpkin have the same protective strategy against photo-damage, reversible down-regulation of PS2, but two different mechanisms, namely the reversible down-regulation is related to the dissociation of LHC2 in soybean leaves but not in pumpkin leaves.

Keywords: chlorophyll fluorescence; Cucurbita; D1 protein; dissociation; Glycine; light-harvesting complex 2 (LHC2); polyacrylamide gel electrophoresis; species differences; sucrose density gradient centrifugation

Published: June 1, 2003Show citation

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Zhang, H., & Xu, D. (2003). Different Mechanisms for Photosystem 2 Reversible Down-Regulation in Pumpkin and Soybean Leaves Under Saturating Irradiance. Photosynthetica, 41(2), 177-184. doi: 10.1023/B:PHOT.0000011949.91626.ec.
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