Photosynthetica, 2004 (vol. 42), issue 1

Photosynthetica 2004, 42(1):43-47 | DOI: 10.1023/B:PHOT.0000040568.58103.ca

Photosynthetic Enzymes of the C4 Grass Setaria sphacelata Under Water Stress: A Comparison Between Rapidly and Slowly Imposed Water Deficit

J. Marques Da Silva1, M.C. Arrabaça1
1 Centro de Engenharia Biológica and Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal

Two stress imposing systems were used: a rapid stress developed by allowing excised leaves to loose water by transpiration, and a slow stress developed by withholding watering of potted plants. Carboxylating enzymes reacted differently on both types of stress. Rapid stress increased ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activation, but both activities (initial and total) showed little variation with stress. Under slow stress the activation did not change, although both activities decreased much under stress. Phosphoenolpyruvate carboxylase (PEPC) showed a deep decrease of activity under rapid stress, nevertheless, a certain recovery was found under extreme stress. On the other hand, under slow stress the activity of PEPC showed a linear increase with decreasing relative water content. The ratio between physiological and maximal activity increased slightly under both types of stress. The activity of malic enzyme did not change under rapid stress, and decreased linearly under slow stress.

Keywords: NADP-malic enzyme; phosphoenolpyruvate carboxylase; ribulose-1,5-bisphosphate carboxylase, oxygenase

Published: March 1, 2004Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Da Silva, J.M., & Arrabaça, M.C. (2004). Photosynthetic Enzymes of the C4 Grass Setaria sphacelata Under Water Stress: A Comparison Between Rapidly and Slowly Imposed Water Deficit. Photosynthetica, 42(1), 43-47. doi: 10.1023/B:PHOT.0000040568.58103.ca.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arrabaça, M.C., Bernardes da Silva, A., Marques da Silva, J.: Effect of rapid dehydration on the activity of Rubisco from the C4 grass Paspalum dilatatum.-In: PS2001-Proceedings of the 12th International Congress on Photosynthesis, S17-024. Pp. 1-4. CSIRO Publishing, Collingwood 2001.
    2. Bernardes da Silva, A., Arrabaça, M.C., Marques da Silva, J.: Effect of rapid dehydration on the activity of PEPC from the C4 grass Paspalum dilatatum.-In: PS2001-Proceedings of the 12th International Congress on Photosynthesis, S17-023. Pp. 1-4. CSIRO Publishing, Collingwood 2001.
    3. Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    4. Donkin, M.E., Martin, E.S.: Studies on the properties of carbo-xylating enzymes in the epidermis of Commelina communis.-J. exp. Bot. 31: 357-363, 1980. Go to original source...
    5. Edwards, G.E., Jenkins, C.L.D., Andrews, J.: CO 2 assimilation and activities of photosynthetic enzymes in high chlorophyll fluorescence mutants of maize having low levels of ribulose 1,5-bisphosphate carboxylase.-Plant Physiol. 86: 533-539, 1988. Go to original source...
    6. Foyer, C.H., Vanacker, H., Gómez, L., Harbinson, J.: Regula-tion of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: a review.-Plant Physiol. Biochem. 40: 659-668, 2002. Go to original source...
    7. Jiao, J.-A., Echevarría, C., Vidal, J., Chollet, R.: Protein turn-over as a component in the light/dark regulation of phospho-enolpyruvate carboxylase protein-serine kinase activity in C4 plants.-Proc. nat. Acad. Sci. USA 88: 2712-2715, 1991. Go to original source...
    8. Medrano, H., Escalona, J.M., Bota, J., Gulías, J., Flexas, J.: Regulation of photosynthesis of C3 plants in response to pro-gressive drought: stomatal conductance as a reference pa-rameter.-Ann. Bot. 89: 895-905, 2002. Go to original source...
    9. Ogawa, N., Kai, T., Yabuta, N., Izui, K.: Phosphoenolpyruvate carboxylase of maize leaves: an improved method for purification and reduction of the inhibitory effect of malate by ethylene glycol and bicarbonate.-Plant Cell Physiol. 38: 76-80, 1997. Go to original source...
    10. Ögren, E.: Evaluation of chlorophyll fluorescence as a probe for drought stress in willow leaves.-Plant Physiol. 93: 1280-1285, 1990. Go to original source...
    11. Parry, M.A.J., Andralojc, P.J., Khan, S., Lea, P.J., Keys, A.J.: Rubisco activity: effects of drought stress.-Ann. Bot. 89: 833-839, 2002. Go to original source...
    12. Parry, M.A.J., Delgado, E., Vadell, J., Keys, A.J., Lawlor, D.W., Medrano, H.: Water stress and the diurnal activity of ribulose-1,5-bisphosphate carboxylase in field grown Nico-tiana tabacum genotypes selected for survival at low CO2 concentrations.-Plant Physiol. Biochem. 31: 113-120, 1993.
    13. Perchorowicz, J.T., Raynes, D.A., Jensen, R.G.: Measurement and preservation of the in vivo activation of ribulose 1,5-bis-phosphate carboxylase in leaf extracts.-Plant Physiol. 69: 1165-1168, 1982. Go to original source...
    14. Saccardy, K., Cornic, G., Brulfert, J., Reyss, A.: Effect of drought stress on net CO2 uptake by Zea mays leaves.-Planta 199: 589-595, 1996. Go to original source...
    15. Simon, P.: Differential effects of chilling on the activity of C4 enzymes in two ecotypes of Echinochloa crus-galli from sites of contrasting climates.-Physiol. Plant. 69: 205-210, 1987. Go to original source...
    16. Sokal, R.R., Rohlf, F.J.: Biometry. 3rd-W.H. Freeman, New York 1995.
    17. Vu, J.C.V., Gesch, R.W., Allen, L.H., Boote, K.J., Bowes, G.: CO2 enrichment delays a rapid, drought-induced decrease in RUBISCO small subunit transcript abundance.-J. Plant Physiol. 155: 139-142, 1999. Go to original source...
    18. Webber, A.N., Nie, G.-Y., Long, S.P.: Acclimation of photo-synthetic proteins to rising atmospheric CO2.-Photosynth. Res. 39: 413-425, 1994. Go to original source...
    19. Willeford, K.O., Wedding, R.T.: Oligomerization and regula-tion of higher plant phosphoenolpyruvate carboxylase-Plant Physiol. 99: 755-758, 1992. Go to original source...
    20. Williams, J., Bulman, M.P., Neill, S.J.: Wilt-induced ABA biosynthesis, gene expression and down-regulation of rbcS m-RNA levels in Arabidopsis thaliana.-Physiol. Plant. 91: 177-182, 1994. Go to original source...

    Return to the content