Photosynthetica, 2007 (vol. 45), issue 4

Photosynthetica 2007, 45(4):562-567 | DOI: 10.1007/s11099-007-0096-z

Delayed fluorescence as a direct indicator of diurnal variation in quantum and radiant energy utilization efficiencies of phytoplankton

E. Kurzbaum1,2,*, W. Eckert2, Y. Z. Yacobi2
1 Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
2 The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, Israel

We compared delayed fluorescence (DF) excitation spectrometry with radiocarbon (14C) technique using a monoalgal culture of Chlorella vulgaris grown under natural temperature and irradiance. This was done by monitoring the DF, in parallel to quantum efficiency (QE) and index of radiant energy utilization efficiency (Ψ) as calculated on the basis of carbon uptake measurements by radiocarbon technique. During the diurnal cycle, temperature, irradiance, and chlorophyll (Chl) contents were monitored in the algal culture that was kept in an open transparent plastic tank submerged at the surface of Lake Kinneret, Israel. The DF signal correlated with both the QE (r 2 = 0.869, p<0.01) and Ψ (r 2 = 0.977, p<0.01) during a diurnal cycle. We suggest that, besides the measurement of active Chl and phytoplankton population composition, the DF signal provides additional information on the QE and Ψ in phytoplankton population.

Keywords: Chlorella; chlorophyll; index of radiant energy utilization efficiency (Ψ); photosynthesis

Received: January 30, 2007; Accepted: June 28, 2007; Published: December 1, 2007Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Kurzbaum, E., Eckert, W., & Yacobi, Y.Z. (2007). Delayed fluorescence as a direct indicator of diurnal variation in quantum and radiant energy utilization efficiencies of phytoplankton. Photosynthetica, 45(4), 562-567. doi: 10.1007/s11099-007-0096-z.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Arnold, W., Davidson, J.B.: The identity of the fluorescent and delayed light emission spectra in Chlorella.-J. gen. Physiol. 37: 677-684, 1953.
    2. Arnold, W., Thompson, J.: Delayed light production by blue-green algae, red algae, and purple bacteria.-J. gen. Physiol. 39: 311-318, 1955.
    3. Berman, T., Pollingher, U.: Annual and seasonal variations of phytoplankton, chlorophyll and photosynthesis in Lake Kinneret.-Limnol. Oceanogr. 19: 31-54, 1974. Go to original source...
    4. Bodemer, U.: [Delayed Fluorescence in vivo Analysis of Algal Pigments and its Application in Limnology.]-PhD Thesis. Institut für Limnologie, Mondsee 2002. [In German.]
    5. Bodemer, U.: Variability of phycobiliproteins in cyanobacteria detected by delayed fluorescence excitation spectroscopy and its relevance for determination of phytoplankton composition of natural water samples.-J. Plankton Res. 26: 1147-1162, 2004. Go to original source...
    6. Bodemer, U., Gerhardt, V., Yacobi, Y.Z., Zohary, T., Friedrich, G., Pohlmann, M.: Phytoplankton abundance and composition in fresh-water systems determined by DF excitation spectroscopy and conventional methods.-Arch. Hydrobiol. spec. Issues Advanc. Limnol. 55(Limnology and Lake Management): 87-100, 2000.
    7. Dubinsky, Z.: Light utilization efficiency in natural phytoplankton communities.-In: Falkowski, P.G. (ed.): Primary Productivity in the Sea. Pp. 83-97. Plenum, New York 1980. Go to original source...
    8. Falkowski, P.G.: Light-shade adaptation and assimilation numbers.-J. Plankton Res. 3: 203-216, 1981. Go to original source...
    9. Falkowski, P.G., Raven, J.A.: Aquatic Photosynthesis.-Blackwell, Malden 1997.
    10. Friedrich, G., Gerhardt, V., Bodemer, U., Pohlmann, M.: Phytoplankton composition and chlorophyll concentration in freshwaters: comparison of delayed fluorescence excitation spectroscopy, extractive spectrophotometric method, and Utermöhl method.-Limnologica 28: 328-232, 1998.
    11. Gerhardt, V., Bodemer, U.: Delayed fluorescence excitation spectroscopy: A method for determining phytoplankton composition in limnology and lake management.-Arch. Hydrobiol. spec. Issue Advanc. Limnol. 55: 101-120, 2000.
    12. Gerhardt, V., Bodemer, U., Eckert, W.: Determination of phytoplankton colour classes: online excitation spectroscopy of the delayed fluorescence (DF) of algae.-Verh. int. Verein. Limnol. 29: 306-309, 2005. Go to original source...
    13. Gerhardt, V., Krause, H., Raba, G., Gebhardt, W.: Delayed fluorescence from photosynthetic active phytoplankton in the time range from 1 to 55 seconds.-J. Luminesc. 24/25: 799-802, 1981. Go to original source...
    14. Greene, R.M., Kolber, Z.S., Swift, D.G., Tindale, N.W., Falkowski, P.G.: Physiological limitation of phytoplankton photosynthesis in the eastern equatorial Pacific determined from variability in the quantum yield of fluorescence.-Limnol. Oceanogr. 39: 1061-1074, 1994. Go to original source...
    15. Håkanson, L., Malmaeus, M., Bodemer, U., Gerhardt, V.: Coefficients of variation for chlorophyll, green algae, diatoms, cryptophytes and blue-greens in rivers as a basis for predictive modelling and aquatic management.-Ecol. Model. 169: 179-196, 2003. Go to original source...
    16. Holm-Hansen, O., Lorenzen, C.J., Holmes, R.W.: Fluorometric determination of chlorophyll.-J. Cons. int. Explor. Mer 30: 3-15, 1965. Go to original source...
    17. Honti, M., Istvanovics, V., Osztoics, A.: Measuring and modelling in situ dynamic photosynthesis of various phytoplankton groups.-Int. Verhandl. theor. angew. Limnol. 29: 194-197, 2005.
    18. Istvanovics, V., Honti, M., Osztoics, A., Shafik, M.H., Padisak, J., Yacobi, Y., Eckert, W.: Continuous monitoring of phytoplankton dynamics in Lake Balaton (Hungary) using on-line delayed fluorescence excitation spectroscopy.-Freshwater Biol. 50: 1950-1970, 2005.
    19. Katsumata, M., Koike, T., Nishikawa, M., Kazumura, K., Tsuchiya, H.: Rapid ecotoxicological bioassay using delayed fluorescence in the green alga Pseudokirchneriella subcapitata.-Water Res. 40: 3393-3400, 2006. Go to original source...
    20. Kishino, M., Okami, N., Takahashi, M., Ichimura, S.: Light utilization efficiency and quantum yield of phytoplankton in a thermally stratified sea.-Limnol. Oceanogr. 31: 557-566, 1986. Go to original source...
    21. Kolber, Z., Falkowski, P.G.: Use of active fluorescence to estimate phytoplankton photosynthesis in situ.-Limnol. Oceanogr. 38: 1646-1665, 1993. Go to original source...
    22. Kolber, Z., Prášil, O., Falkowsky, P.G.: Measurements of variable chlorophyll fluorescence using fast repetition rate techniques: defining methodology and experimental protocols.-Biochim. biophys. Acta 1367: 88-106, 1998. Go to original source...
    23. Kroon, B.M.A.: Variability of photosystem II quantum yield and related processes in Chlorella pyrenoidosa (Chlorophyta) acclimated to an oscillating light regime simulating a mixed photic zone.-J. Phycol. 30: 841-852, 1994. Go to original source...
    24. Kurzbaum, E.: [Delayed Fluorescence as a Measure for Photosynthetic Traits and Monitoring Tool for Phytoplankton Populations.]-M.Sc. Thesis. Tel-Aviv University, 2006. [In Hebrew.]
    25. Lorenzen, C.J.: Determination of chlorophyll and pheo-pigments: spectrophotometric equations.-Limnol. Oceanogr. 12: 343-346, 1967. Go to original source...
    26. Masojídek, J., Torzillo, G., Koblížek, M., Kopecký, J., Bernardini, P., Sacchi, A., Komenda, J.: Photoadaptation of two members of the Chlorophyta (Scenedesmus and Chlorella) in laboratory and outdoor cultures: changes in chlorophyll fluorescence quenching and the xanthophyll cycle.-Planta 209: 126-135, 1999. Go to original source...
    27. Mellvig, S., Tillberg, J.-E.: Transient peaks in the delayed luminescence from Scenedesmus obtusiusculus induced by phosphorus starvation and carbon dioxide deficiency.-Physiol. Plant. 68: 180-188, 1986. Go to original source...
    28. Monti, M., Zrimec, A., Beran, A., Berden Zrimec, M., Drinovec, L., Kosi, G., Tamberlich, F.: Delayed luminescence of Prorocentrum minimum under controlled conditions.-Harmful Algae 4: 643-650, 2005. Go to original source...
    29. Moss, B.: The influence of environmental factors on the distribution of freshwater algae: an experimental study. I. Introduction and the influence of calcium concentration.-J. Ecol. 60: 917-932, 1972. Go to original source...
    30. Naddafi, R., Pettersson, K., Eklöv, P.: The effect of seasonal variation in selective feeding by zebra mussels (Dreissena polymorpha) on phytoplankton community composition.-Freshwater Biol. 52: 823-842, 2007. Go to original source...
    31. Odum, E.P.: Fundamentals of Ecology.-Saunders, Philadelphia 1971.
    32. Schreiber, U.: Detection of rapid induction kinetics with a new type of high frequency modulated chlorophyll fluorometer.-Photosynth. Res. 9: 261-272, 1986. Go to original source...
    33. Steemann-Nielsen, E.: The use of radioactive carbon (14C) for measuring organic production in the sea.-J. Cons. int. Explor. Mer 18: 117-140, 1952. Go to original source...
    34. Strehler, B.L., Arnold, W.: Light production by green plants.-J. gen. Physiol. 34: 809-820, 1951. Go to original source...
    35. Suggett, D.J., Oxborough, K., Baker, N.R., MacIntyre, H.L., Kana, T.M., Geider, R.J.: Fast repetition rate and pulse amplitude modulation chlorophyll a fluorescence measurements for assessment of photosynthetic electron transport in marine phytoplankton.-Eur. J. Phycol. 38: 371-384, 2003. Go to original source...
    36. Torsten, J., Schreiber, U., Kirchesch, V., Langner, U., Wilhelm, C.: Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits.-Photosynth. Res. 83: 343-361, 2005.
    37. Wang, C.L., Xing, D., Chen, Q.: A novel method for measuring photosynthesis using delayed fluorescence of chloroplast.-Biosensors Bioelectronics 20: 454-459, 2004. Go to original source...
    38. Wilhelm, C., Becker, A., Toepel, J., Vieler, A., Rautenberger, R.: Photophysiology and primary production of phytoplankton in freshwater.-Physiol. Plant. 120: 347-354, 2004. Go to original source...
    39. Wiltshire, K.H., Harsdorf, S., Smidt, B., Blöcker, G., Reuter, R., Schroeder, R.F.: The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary and the German Bight: A comparison of high-performance liquid chromatography, delayed fluorescence and prompt fluorescence methods.-J. exp. mar. Biol. Ecol. 222: 113-131, 1998. Go to original source...
    40. Yacobi, Y.Z., Gerhardt, V., Gonen-Zurgil, Y., Sukenik, A.: Delayed fluorescence excitation spectroscopy: A rapid method for qualitative and quantitative assessment of natural population of phytoplankton.-Water Res. 32: 2577-2582, 1998. Go to original source...
    41. Zrimec, A., Drinovec, L., Berden-Zrimec, M.: Influence of chemical and physical factors on long-term delayed fluorescence in Dunaliella tertiolecta.-Electromagnetic Biol. Med. 24: 309-318, 2005.

    Return to the content