Photosynthetica, 2018 (vol. 56), issue 2

Photosynthetica 2018, 56(2):557-566 | DOI: 10.1007/s11099-017-0712-5

The positive roles of exogenous putrescine on chlorophyll metabolism and xanthophyll cycle in salt-stressed cucumber seedlings

R. N. Yuan1, S. Shu1, S. R. Guo1,2,*, J. Sun1,2, J. Q. Wu1
1 Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, China
2 Nanjing Agricultural University (Suqian) Academy of Protected Horticulture, Suqian, China

The effects of foliar spray of putrescine (Put; 8 mM) on chlorophyll (Chl) metabolism and xanthophyll cycle in cucumber seedlings were investigated under saline conditions of 75 mM NaCl. Exogenous Put promoted the conversion of uroporhyrinogen III to protoporphyrin IX and alleviated decreases in Chl contents and in a size of the xanthophyll cycle pool under salt stress. Moreover, the Put treatment reduced the activities of uroporphyrinogen III synthase, chlorophyllase, and Mg-dechelatase and downregulated the transcriptional levels of glutamyl-tRNA reductase, 5-aminolevulinate dehydratase, uroporphyrinogen III synthase, uroporphyrinogen III decarboxylase, and chlorophyllide a oxygenase, but significantly increased the expression levels of non-yellow coloring 1-like, pheide a oxygenase, red chlorophyll catabolite reductase, and violaxanthin de-epoxidase. Taken together, these results suggest that Put might improve Chl metabolism and xanthophyll cycle by regulating enzyme activities and mRNA transcription levels in a way that improved the salt tolerance of cucumber plants.

Keywords: chlorophyll biosynthesis; chlorophyll degradation; de-epoxidation; energy dissipation; light-harvesting complex; pigments

Received: May 18, 2016; Accepted: November 14, 2016; Published: June 1, 2018Show citation

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Yuan, R.N., Shu, S., Guo, S.R., Sun, J., & Wu, J.Q. (2018). The positive roles of exogenous putrescine on chlorophyll metabolism and xanthophyll cycle in salt-stressed cucumber seedlings. Photosynthetica, 56(2), 557-566. doi: 10.1007/s11099-017-0712-5.
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