Photosynthetica, 2011 (vol. 49), issue 1

Photosynthetica 2011, 49(1):37-42 | DOI: 10.1007/s11099-011-0005-3

Damaging mechanisms of chilling- and salt stress to Arachis hypogaea L. leaves

L. Q. Qin1,2,3, L. Li3, C. Bi4, Y. L. Zhang1,2, S. B. Wan1,2, J. J. Meng1,2, Q. W. Meng3, X. G. Li1,2,*
1 High-Tech Research Center, Shandong Academy of Agricultural Sciences and Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Ji'nan, China
2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, the People 's Republic of China, Ji'nan, China
3 College of Life Sciences, Shandong Agricultural University, Tai'an, China
4 Shandong University, Ji'nan, China

To investigate damaging mechanisms of chilling and salt stress to peanut (Arachis hypogaea L.) leaves, LuHua 14 was used in the present work upon exposure to chilling temperature (4°C) accompanied by high irradiance (1,200 μmol m-2 s-1) (CH), salt stress accompanied by high irradiance (1,200 μmol m-2 s-1) (SH), and high-irradiance stress (1,200 μmol m-2 s-1) at room temperature (25°C) (NH), respectively. Additionally, plants under low irradiance (100 μmol m-2 s-1) at room temperature (25°C) were used as control plants (CK). Relative to CK and NH treatments, both the maximal photochemical efficiency of PSII (Fv/Fm) and the absorbance at 820 nm decreased greatly in peanut leaves under CH and SH stress, which indicated that severe photoinhibition occurred in peanut leaves under such conditions. Initial fluorescence (Fo), 1 - qP and nonphotochemical quenching (NPQ) in peanut leaves significantly increased under CH- and SH stress. Additionally, the activity of superoxide dismutase (SOD), one of the key enzymes of water-water cycle, decreased greatly, the accumulation of malondialdehyde (MDA) and membrane permeability increased. These results suggested that damages to peanut photosystems might be related to the accumulation of reactive oxygen species (ROS) induced by excess energy, and the water-water cycle could not dissipate energy efficiently under the stress of CH and SH, which caused the accumulation of ROS greatly. CH and SH had similar damaging effects on peanut photosystems, except that CH has more severe effects. All the results showed that CH- and SH stress has similar damaging site and mechanisms in peanut leaves.

Keywords: chilling temperature; peanut; photoinhibition; reactive oxygen species; salt stress

Received: June 20, 2010; Accepted: November 21, 2010; Published: March 1, 2011Show citation

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Qin, L.Q., Li, L., Bi, C., Zhang, Y.L., Wan, S.B., Meng, J.J., Meng, Q.W., & Li, X.G. (2011). Damaging mechanisms of chilling- and salt stress to Arachis hypogaea L. leaves. Photosynthetica, 49(1), 37-42. doi: 10.1007/s11099-011-0005-3.
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    References

    1. Allen, D.J., Ort, D.R.: Impacts of chilling temperatures on photosynthesis in warm-climate plants. - Trends Plant Sci. 6: 36-41, 2001. Go to original source...
    2. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of photosystem II. Inactivation, protein damage and turnover. - Biochim. Biophys. Acta 1143: 113-134, 1993. Go to original source...
    3. Asada, K.: The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. - Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 601-639, 1999. Go to original source...
    4. Boyer, J.S.: Plant productivity and environment. - Science 218: 443-448, 1982. Go to original source...
    5. Dat, J., Vandenabeele, S., Vranová, E., Van Montagu, M., Inzé, D., Van Breusegem, F.: Dual action of the active oxygen species during plant stress responses. - Cell. Mol. Life Sci. 57: 779-795, 2000. Go to original source...
    6. Demmig-Adams, B., Adams, W.W., III: The role of xanthophyll cycle carotenoids in the protection of photosynthesis. - Trends Plant Sci. 1: 21-26, 1996. Go to original source...
    7. Dhindsa, R.S., Plumb-Dhindsa P., Athorpe, D.T.: Leaf senescence: correlated with increased levels of membrane permeability and lipid pemxidation and decreased levels of superoxide dismutase and catalase. - J. Exp. Bot. 32: 93-101, 1981. Go to original source...
    8. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases. I. Occurrence in higher plants. - Plant Physiol. 59: 309-314, 1977. Go to original source...
    9. Havaux, M., Strasser, R.J., Greppin. H.: A theoretical and experimental analysis of the qP and qN coefficients of chlorophyll fluorescence quenching and their relation to photochemical and nonphotochemical events. - Photosynth. Res. 27: 41-55, 1991. Go to original source...
    10. Ketring, D.L.: Light effects on development of all indeterminate plant. - Plant Physio1. 64: 665-667, 1979. Go to original source...
    11. Lee, S.H., Singh, A.P., Chung, G.C.: Rapid accumulation of hydrogen perodixe in cucumber roots due to exposure to low temperature appears to mediate in water transport. - J. Exp. Bot. 55: 1733-1741, 2004. Go to original source...
    12. Li, X.G., Duan, W., Meng, Q.W., Zou, Q., Zhao, S.J.: The function of chloroplastic NAD(P)H dehydrogenase in tobacco during chilling stress under low irradiance. - Plant Cell Physiol. 45: 103-108, 2004a. Go to original source...
    13. Li, X.G., Bi, Y.P., Zhao, S.J., Meng, Q.W., He, Q.W., Zou, Q.: Effects of short-term chilling stress on the photosystems and chloroplast ultrastructure in sweet pepper. - Agr. Sci. China 4: 429-435, 2005.
    14. Li, X.G., Li, J.Y., Zhao, J.P., Xu, P.L., He, Q.W.: Xanthophyll cycle and inactivation of photosystem 2 reaction centers alleviating reducing pressure to photosystem 1 in morning glory leaves upon exposure to a short-term high irradiance. - J. Integr. Plant Biol. 49: 1047-1053, 2007. Go to original source...
    15. Li, X.-G., Wang, X.-M., Meng, Q.-W., Zou, Q.: Factors of limiting photosynthetic recovery in sweet pepper leaves after short-term chilling stress under low irradiance. - Photosynthetica 42: 257-262, 2004b. Go to original source...
    16. Lindahl, M., Spetea, C., Hundal, T., Oppenheim, A.B., Adam, Z., Andersson, B.: The thylakoid FtsH protease plays a role in the light-induced turnover of the photosystem II D1 protein. - Plant Cell 12: 419-431, 2000. Go to original source...
    17. Lyons, J.K., Chapman, E.A.: Membrane phase changes in chilling-sensitive Vigna radiata and their significance to growth. - Aust. J. Plant Physiol. 3: 291, 1976.
    18. Munns, R., James, R.A., Läuchli, A.: Approaches to increasing the salt tolerance of wheat and other cereals. - J. Exp. Bot. 57: 1025-1043, 2006. Go to original source...
    19. Murata, N., Mohanty, P.S., Hayashi, H., Papageorgiou, G.C.: Glycinebetaine stabilizes the association of extrinsic proteins with the photosynthetic oxygen evolving complex. - FEBS Lett. 296: 187-189, 1992. Go to original source...
    20. Ögren, E., Öquist, G.: Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. III. Chlorophyll fluorescence at 77 K. - Physiol. Plant. 62: 193-200, 1984. Go to original source...
    21. Ohnishi, N., Murata, N.: Glycinebetaine counteracts the inhibitory effects of salt stress on the degradation and synthesis of D1 protein during photoinhibition in Synechococcus sp. PCC 7942. - Plant Physiol. 141: 758-765, 2006. Go to original source...
    22. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light. - Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    23. Rao, L.J., Zhao, Z.P.: [The effect of shade degree and duration on development and yield of peanut.] - Abroad Agron. (Agr. Wealth) 2: 35, 1989. [In Chin.]
    24. Schansker, G., Srivastava, A., Govindjee, Strasser R.J.: Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves. - Function. Plant Biol. 30: 785-796, 2003.
    25. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. - In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis, Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    26. Sonoike, K., Terashima, I.: Mechanism of photosystem-I photoinhibition in leaves of Cucumis sativus L. - Planta 194: 287-293, 1994. Go to original source...
    27. Sudhir, P., Murthy, S.D.S.: Effects of salt stress on basic processes of photosynthesis. - Photosynthetica 42: 481-486, 2004. Go to original source...
    28. van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. - Photosynth. Res. 25: 147-150, 1990. Go to original source...
    29. Vonshak, A., Kancharaksa, N., Bunnag, B. and Tanticharoen, M.: Role of light and photosynthesis on the acclimation process of the cyanobacterium Spirulina platensis to salinity stress. - J. Appl. Phycol. 8: 119-124, 1996. Go to original source...
    30. Xu, C.C., Jeon, Y.A., Lee, C.H.: Relative contributions of photochemical and non-photochemical routes to excitation energy dissipation in rice and barley illuminated at a chilling temperature. - Physiol. Plant. 107: 447-453, 1999. Go to original source...
    31. Xu, D.Q., Wu, S.: Three phases of dark-recovery course from photoinhibition resolved by the chlorophyll fluorescence analysis in soybean leaves under field conditions. - Photosynthetica 32: 417-423, 1996.
    32. Yao, A.A., Bernard, W., Philippe, T.: Effect of protective compounds on the survival, electrolyte leakage, and lipid degradation of freeze-dried Weissella paramesenteroides LC11 during storage. - J. Microbiol. Biotechnol. 19: 810-817, 2009. Go to original source...
    33. Zhao, S.J., Xu, C.C., Zou, Q., Meng, Q.W.: [Improvements of method for measurement of malondialdehyde in plant tissues.] - Plant Physiol. Commun. 30: 207-210, 1994. [In Chin.]

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