Photosynthetica, 2019 (vol. 57), issue 1

Photosynthetica 2019, 57(1):40-46 | DOI: 10.32615/ps.2019.012

Diurnal changes in the delayed fluorescence response of an ambient light-excited green alga

W. ECKERT1, F. LEUNERT2, Y.Z. YACOBI1, J. KÖHLER2, E. KURZBAUM3,4
1 The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Haifa, Israel
2 Department of Ecosystem, Leibniz Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany
3 Shamir Research Institute, University of Haifa, P.O. Box 97, Qatzrin, Israel
4 Department of Geography and Environmental Studies, University of Haifa, Mount Carmel, Haifa 3498838, Israel

Delayed fluorescence (DF) is a characteristic feature of light-excited plant cells caused by the back-reaction of electrons through the electron transport chain. Targeting the response of light-adapted green algae to diurnal light changes, the present study presents novel results of DF measurements in the absence of an artificial excitation light source. Based on a linear relationship between the DF counts and light intensities from 0.15 to 0.65 W m-2 during daybreak, we estimated an initial algal response to light intensities of 0.01 W m-2. Dissolved oxygen concentrations began to increase at 1.0 W m-2. A noon depression similar to that reported for prompt fluorescence occurred above 100 W m-2. Our results from multiple day-night cycles emphasize that the DF response is a function of the chlorophyll concentration and of a rapid light adaptation. The DF counts alone cannot provide a reliable unambiguous measure of photosynthetic activity.

Keywords: Chlorella vulgaris; chlorophyll fluorescence; diurnal light cycle; phytoplankton.

Received: December 18, 2017; Accepted: June 14, 2018; Prepublished online: December 5, 2018; Published: January 30, 2019Show citation

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ECKERT, W., LEUNERT, F., YACOBI, Y.Z., KÖHLER, J., & KURZBAUM, E. (2019). Diurnal changes in the delayed fluorescence response of an ambient light-excited green alga. Photosynthetica, 57(1), 40-46. doi: 10.32615/ps.2019.012.
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    References

    1. Avron M., Schreiber U.: Properties of ATP-induces chlorophyll luminescence in chloroplasts. - Biochemistry 546: 448-454, 1979.
    2. Berden-Zrimec M., Drinovec L., Zrimec A. et al: Delayed fluorescence in algal growth inhibition tests. - Cent. Eur. J. Biol. 2: 169-181, 2007. Go to original source...
    3. Berden-Zrimec M., Drinovec L., Zrimec, A.: Delayed fluores-cence. - In: Suggett D.J., Borowitzka M.A., Prášil O. (ed.): Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Pp. 293-309. Springer, Dordrecht 2010. Go to original source...
    4. Bertsch W.F.: Two photoreactions in photosynthesis: evidence from the delayed light emission of chlorella. - P. Natl. Acad. Sci. USA 48: 2000-2004, 1962.
    5. Flameling I.A., Kromkamp J.: Light dependence of quantum yields for PSI1 charge separation and oxygen evolution in eucaryotic algae. - Limnol. Oceanogr. 43: 284-297, 1998. Go to original source...
    6. Gerhardt V., Bodemer U.: Delayed fluorescence excitation spectrocopy: a method for determining phytoplankton composition. - Arch. Hydrobiol. Spec. Issues Adv. Limnol. 55: 101-120, 2000.
    7. Gerhardt V., Bodemer U.: Delayed fluorescence excitation spectroscopy: A method for automatic determination of phytoplankton composition of freshwater and sediments (interstital) of algal composition of benthos. - Limnologica 28: 313-322, 1998.
    8. Gerhardt V., Bodemer U., Eckert W.: Determination of phyto-plankton colour classes: Online excitation spectroscopy of the delayed fluorescence (DF) of algae. - Verh. Internat. Verein. Limnol. 29: 306-309, 2005.
    9. Gerhardt V., Krause H., Raba G. et al: Delayed fluorescence from photosynthetic active phytoplankton in the time-range from 1 to 55 seconds. - J. Lumin. 24-25: 799-802. 1981. Go to original source...
    10. Gilbert M., Domin A., Becker A. et al: Estimation of primary productivity by chlorophyll a in vivo fluorescence in fresh-water phytoplankton. - Photosynthetica 38: 111-126, 2000. Go to original source...
    11. Goltsev V., Zaharieva I., Chernev P. et al: Delayed fluorescence in photosynthesis. - Photosynth. Res. 101: 217-232, 2009. Go to original source...
    12. Han B.-P., Virtanen M., Koponen J. et al: Effect of photoinhibition on algal photosynthesis: a dynamic model. - J. Plankton Res. 22: 865-885, 2000. Go to original source...
    13. Hanelt D., Nultsch W.: Field studies of photoinhibition show non-correlations between oxygen and fluorescence measurements in the arctic red alga Palmaria palmata. - J. Plant Physiol. 145: 31-38, 1995. Go to original source...
    14. Hanelt D., Kuppertz K., Nultsch W.: Daily course of photo-synthesis and photoinhibition in marine macroalgae investigated in the laboratory and feild. - Mar. Ecol. Prog. Ser. 97: 31-37, 1993. Go to original source...
    15. Henley W.J., Levavasseur G., Franklin L. et al.: Diurnal responses of photosynthesis and fluorescence in Ulva rotundata acclimated to sun and shade in outdoor culture. - Mar. Ecol. Prog. Ser. 75: 19-28, 1991. Go to original source...
    16. Holm-Hansen O., Lorenzen C.J., Holmes R.W. et al.: Fluoro-metric determination of chlorophyll. - ICES J. Mar. Sci. 30: 3-15, 1965. Go to original source...
    17. Istvánovics V., Honti M., Osztoics A. et al: Continuous monitoring of phytoplankton dynamics in Lake Balaton (Hungary) using on-line delayed fluorescence excitation spectroscopy. - Freshwater Biol. 50: 1950-1970, 2005.
    18. Jursinic P.: Delayed fluorescence: current concepts and status. - In: Govindjee, Amesz J., Fork D.C. (ed.): Light Emission by Plants and Bacteria. Pp. 292-328. Academic Press, San Diego 1986. Go to original source...
    19. Katsumata M., Koike T., Nishikawa M. et al.: Rapid eco-toxicological bioassay using delayed fluorescence in the green alga Pseudokirchneriella subcapitata. - Water Res. 40: 3393-3400, 2006. Go to original source...
    20. Krause H., Dirnhofer P., Gerhardt V.: The application of delayed fluorescence of phytoplankton in ecology. - Arch. Hydrobiol. Beih. Ergebn. Limnol. 29: 55-62, 1987.
    21. Kretsch G., Gerhardt V.: Numerical analysis of delayed fluorescence of algae. - Arch. Hydrobiol. Beih. Ergebn. Limnol. 29: 47-54, 1987.
    22. Kurzbaum E.: Delayed fluorescence as a simple and rapid measurment tool for active chlorophyll concentration, phytoplankton compositions and a possible tool for monitoring photosynthetic traits of phytoplankton. - In: Kersey W., Munger S.P. (ed.): Marine Phytoplankton. Pp. 351-354. Nova Science, New York 2009.
    23. Kurzbaum E., Beer S., Eckert W.: Alterations in delayed and direct phytoplankton fluorescence in response to the diurnal light cycle. - Hydrobiologia 639: 197-203, 2010. Go to original source...
    24. Kurzbaum E., Eckert W., Yacobi Y.Z.: Delayed fluorescence as a direct indicator of diurnal variation in quantum and radiant energy utilization efficiencies of phytoplankton. - Photosynthetica 45: 562-567, 2007. Go to original source...
    25. Leunert F., Grossart H.-P., Gerhardt V. et al.: Toxicant induced changes on delayed fluorescence decay kinetics of cyanobacteria and green algae: A rapid and sensitive biotest. - PLoS ONE 8: e63127, 2013. Go to original source...
    26. Melcarek P.K., Brown G.N.: Effects of chill stress on prompt and delayed chlorophyll fluorescence from leaves. - Plant Physiol. 60: 822-825, 1977 Go to original source...
    27. Mellvig S., Tillberg J.-E.: Transient peaks in the delayed luminescence from Scenedesmus obtusiusculus induced by phosphorus starvation and carbon dioxide deficiency. - Physiol. Plantarum 68: 180-188, 1986. Go to original source...
    28. Monti M., Zrimec A., Beran A. et al.: Delayed luminescence of Prorocentrum minimum under controlled conditions. - Harmful Algae 4: 643-650, 2005. Go to original source...
    29. Moss B.: The influence of environmental factors on the distribution of freshwater algae: an experimental study: I. Introduction and the influence of calcium concentration. - J. Ecol. 60: 917-932, 1972. Go to original source...
    30. Strehler B., Arnold W.: Light production by green plants. - J. Gen. Physiol. 34: 809-820, 1951. Go to original source...
    31. Suggett D.J., Maberly S.C., Geider R.J.: Gross photosynthesis and lake community metabolism during the spring phytoplankton bloom. - Limnol. Oceanogr. 51: 2064-2076, 2006. Go to original source...
    32. Suggett D.J., Oxborough K., Baker N.R. et al.: Fast repetition rate and pulse amplitude modulation chlorophyll a fluorescence measurements for assessment of photosynthetic electron transport in marine phytoplankton. - Eur. J. Phycol. 38: 371-384, 2003. Go to original source...
    33. Underwood G.: Adaptations of tropical marine microphytobenthic assemblages along a gradient of light and nutrient availability in Suva Lagoon, Fiji. - Eur. J. Phycol. 37: 449-462, 2002. Go to original source...
    34. Wang C., Xing D., Chen Q.: A novel method for measuring photosynthesis using delayed fluorescence of chloroplast. - Biosens. Bioelectron. 20: 454-459, 2004. Go to original source...
    35. Wilhelm C., Becker A., Toepel J. et al.: Photophysiology and primary production of phytoplankton in freshwater. - Physiol. Plantarum. 120: 347-357, 2004. Go to original source...
    36. Yacobi Y.Z., Gerhardt V., Gonen-Zurgil Y. et al.: Delayed fluorescence excitation spectroscopy: A rapid method for qualitative and quantitative assessment of natural population of phytoplankton. - Water Res. 32: 2577-2582, 1998. Go to original source...

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