Photosynthetica 2011, 49(3):435 | DOI: 10.1007/s11099-011-0054-7

Effects of elevated temperature on photosynthesis in desert plant Alhagi sparsifolia S

W. Xue^1,2,3, X. Y. Li^2,3,*, L. S. Lin^1,3, Y. J. Wang^1,2,3, L. Li^1,2,3

¹ Xinjiang Institute of Ecology and Geography, State Key Laboratory of Desert and Oasis Ecology, Chinese Academy of Sciences, Urumqi, China

² Graduate University of Chinese Academy of Sciences, Beijing, China

³ Cele National Station of Observation & Research for Desert Grassland Ecosystem, Cele, Xinjiang, China

Most plants growing in temperate desert zone exhibit brief temperature-induced inhibition of photosynthesis at midday in the summer. Heat stress has been suggested to restrain the photosynthesis of desert plants like Alhagi sparsifolia S. It is therefore possible that high midday temperatures damage photosynthetic tissues, leading to the observed inhibition of photosynthesis. In this study, we investigated the mechanisms underlying heat-induced inhibition of photosynthesis in A. sparsifolia, a dominant species found at the transition zone between oasis and sandy desert on the southern fringe of the Taklamakan desert. The chlorophyll (Chl) a fluorescence induction kinetics and CO₂ response curves were used to analyze the thermodynamic characters of both photosystem II (PSII) and Rubisco after leaves were exposed to heat stress. When the leaves were heated to temperatures below 43°C, the initial fluorescence of the dark-adapted state (F_o), and the maximum photochemical efficiency of PSII (F_v/F_m), the number of active reaction centers per cross section (RCs) and the leaf vitality index (PI) increased or declined moderately. These responses were reversed, however, upon cooling. Moreover, the energy allocation in PSII remained stable. The gradual appearance of a K point in the fluorescence curve at 48°C indicated that higher temperatures strongly impaired PSII and caused irreversible damage. As the leaf temperature increased, the activity of Rubisco first increased to a maximum at 34°C and then decreased as the temperature rose higher. Under high-temperature stress, cell began to accumulate oxidative species, including ammoniacal nitrogen, hydrogen peroxide (H₂O₂), and superoxide (O₂ ^.-), suggesting that disruption of photosynthesis may result from oxidative damage to photosynthetic proteins and thylakoid membranes. Under heat stress, the biosynthesis of nonenzyme radical scavenging carotenoids (Cars) increased. We suggest that although elevated temperature affects the heat-sensitive components comprising of PSII and Rubisco, under moderately high temperature the decrease in photosynthesis is mostly due to inactivation of dark reactions.

Keywords: Alhagi sparsifolia S; elevated temperature; oxidative species; photosystem II; Rubisco activity

Received: November 9, 2010; Accepted: July 4, 2011; Published: September 1, 2011Show citation