Biologia plantarum - Latest articles
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Imazamox detoxification and recovery of plants after application of imazamox to an imidazolinone resistant sunflower hybridOriginal Papers
D. BALABANOVA, T. REMANS, A. CUYPERS, J. VANGRONSVELD, A. VASSILEV
Biologia plantarum 64: 335-342, 2020 | DOI: 10.32615/bp.2019.150
Imidazolinone herbicides combined with imidazolinone resistant (IMI-R) crops provide a tool for solving the important problem of the occurrence of weeds during the early growth stages of sunflower. These herbicides inhibit the synthesis of branched chain amino acids by interrupting the key enzyme acetohydroxyacid synthase (AHAS). We studied the imazamox detoxification in an IMI-R sunflower hybrid together with plant growth and photosynthetic performance. Inhibition of photosynthesis and growth were observed as initial effects of imazamox application. A slight decrease in AHAS activity was also noticed. These effects disappeared within two weeks after...
The classification of tetraploid wheat by phylogenetic and cytogenetic analysesOriginal Papers
S.-Y. LI, L. XI, H.-J. LIU, W. ZHU, L.-L XU, Y. WANG, J. ZENG, X. FAN, L.-N. SHA 1,2, H.-Q. ZHANG, W.-L. QI, G.-Y. CHEN, Y.-H. ZHOU, H.-Y. KANG
Biologia plantarum 64: 324-334, 2020
Tetraploid wheat (Triticum turgidum L.) is an important species within the genus Triticum and harbors many desirable agronomic traits. The classification, origin, and evolution of tetraploid wheat remain confused and controversial, resulting in useless germplasm resources. Two classification systems for tetraploid wheat are widely used: 1) tetraploid wheat comprises two species; 2) all forms of tetraploid wheat are classified as one species. The present study aimed to reassess the classification of tetraploid wheat using phylogenetic analysis of nuclear rDNA internal transcribed spacer region (ITS) sequence data, fluorescence...
Effect of virus inducible cis-element insertion on transcription properties of improved GWSF promoter in Arabidopsis thalianaBrief Communications
Z.C. HUANG, H. LI
Biologia plantarum 64: 320-323, 2020 | DOI: 10.32615/bp.2020.032
An ideal synthetic promoter can accurately regulate gene expression and the minimal cauliflower mosaic virus 35S promoter (GWSF) is an ideal synthetic pathogen-inducible promoter (SPIP) with several advantages. Three modified SPIPs, named as VGWSF, GWVSF, and GWSFV according to the arrangement of cis-elements, were optimized by inserting the dimer of a virus inducible cis-element (TTGGGAAGGAATTTCCTACT, V-box) upstream, midstream, or downstream the GWSF sequence. The three promoters were used to replace the cauliflower mosaic virus 35S promoter in the plasmid pBI121 in order to control the expression of the β-glucuronidase...
Cloning and functional characterization of the β-amyrin synthase genefrom Bupleurum chinenseOriginal Papers
J.-C. LI, C. WANG, W.-T. QI, C.-L. LIU
Biologia plantarum 64: 314-319, 2020 | DOI: 10.32615/bp.2020.008
Bupleurum chinense DC., a source of the traditional Chinese medicine Bupleuri Radix, is rich in triterpenoid saikosaponins with high pharmacological activities. The enzyme β-amyrin synthase (BAS), which converts the precursor 2,3-oxidosqualene to produce the triterpene skeleton, is crucial for the biosynthesis of triterpenoid saponins. In this study, we cloned the full-length sequence of the BAS gene from B. chinense, conducted a bioinformatics analysis, and expressed it in Saccharomyces cerevisiae to investigate its function. The cDNA of β-amyrin synthase (BcBAS, GenBank accession number: MN186093) cloned...
Silicon alleviates salt and drought stress of Glycyrrhiza uralensis plants by improving photosynthesis and water statusOriginal Papers
W.J. ZHANG, X.J. ZHANG, D.Y. LANG, M. LI, H. LIU, X.H. ZHANG
Biologia plantarum 64: 302-313, 2020 | DOI: 10.32615/bp.2019.136
Silicon has been widely reported to have a beneficial effect on improving plant tolerance to biotic and abiotic stresses. However, the mechanisms of Si in mediating responses to simultaneous salt and drought stresses are still poorly understood. Glycyrrhiza uralensis Fisch. is classified as a non-Si accumulator and suffered from salt and drought stresses. In this study, we investigated the long-term application of Si on Si content in G. uralensis roots, stems and leaves, leaf anatomy, ultrastructure, chlorophyll (Chl) content, gas exchange characteristics, relative water content, and growth of two-year-old plants under different salt...
Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen toleranceOriginal Papers
M. YAN, J. LUO, L. LIANG, L. CHEN, Y.Y. CAO, Y.H. CHEN, X.Y. ZHU, C.M. YU
Biologia plantarum 64: 289-301, 2020 | DOI: 10.32615/bp.2019.093
Maize is an important crop and its nitrogen use efficiency (NUE) has been an issue for plant scientists and breeders for many years. To meet the demand of genetic diversity in cultivating local elite maize cultivars, researchers need to isolate germplasms with a high NUE. In this study, 30 maize inbred lines were screened under hydroponic conditions, and three inbred lines that tolerate low nitrogen concentration were identified. A comparative analysis of genes involved in N uptake, transport, and assimilation between two lines with different tolerances revealed that the low-nitrogen-tolerant inbred line MBST exhibited higher expressions of nitrate...
Isolation and expression profiles of class III PRX gene family under drought stress in Camellia sinensisOriginal Papers
H.J. LI, H.B. WANG, Y. CHEN, Q.P. MA, Z. ZHAO, X.H. LI, X. CHEN
Biologia plantarum 64: 280-288, 2020 | DOI: 10.32615/bp.2019.120
The class III PRX family is a class of heme-containing oxidases and plays important roles in response to abiotic stress in plants. The responses to abiotic stresses could be regulated by phytohormones like abscisic acid (ABA) and methyl jasmonate (MeJA). In this research, 11 CsPRXs genes in tea plant (Camellia sinensis) were cloned and analyzed. Based on the similarity of the sequences, they were classified into 5 sub-groups. According to the results of reverse transcription PCR, CsPRX55 presented the highest expression in roots compared to stems and leaves of both tea cultivars LJ43 and Baiye 1. Besides, the expressions of CsPRX12...
Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germinationOriginal Papers
D. KUMAR, P.K. DAS, B.K. SARMAH
Biologia plantarum 64: 244-252, 2020 | DOI: 10.32615/bp.2019.128
Flood in rice fields at the time of seed sowing and early seedling establishment causes extensive crop loss due to the inability of the seeds to tolerate and overcome submergence. In the present study, rice genotypes from Assam, India tolerant to hypoxia during germination were identified through systematic screening of deep-water rice genotype collection from flood-prone ecosystem of Brahmaputra river valley. The difference in tolerance to hypoxia during germination within the species provides scope for identification of novel hypoxia-responsive factors involved in tolerance as mechanism of tolerance may not be conserved across tolerant germplasms....
Salt tolerance of potato genetically engineered with the Atriplex canescens BADH geneOriginal Papers
A. ALI, Q. ALI, M.S. IQBAL, I.A. NASIR, X. WANG
Biologia plantarum 64: 271-279, 2020 | DOI: 10.32615/bp.2019.080
Potato (Solanum tuberosum L.) is among the top staple foods in the world, and salinity adversely affects its yield and quality. To improve salt tolerance in potato, the present study is focused on the Agrobacterium-mediated transformation of potato by the Atriplex canescens betaine aldehyde dehydrogenase (BADH) gene driven by single, double, and triple CaMV 35S promoters. The study led first to the detection of seven lines containing the BADH gene followed by the identification of T-DNA insertions via DNA hybridization and enzyme-linked immunosorbent assays. The salt tolerance was found to be promoter dependent,...
Overexpression of a stilbene synthase gene from Fallopia multiflora causes trans-piceid accumulation in transgenic ArabidopsisBrief Communications
S. SHENG, W. XIA, W. RUI, W. ZHAO, T. SHE, J. XIE, S. ZHAO, L. CHEN
Biologia plantarum 64: 266-270, 2020 | DOI: 10.32615/bp.2019.152
Fallopia multiflora, also known as Polygonum multiflorum, is a traditional Chinese medicine with stilbenes as the main characteristic components. We previously isolated the presumed stilbene synthase gene FmSTS1 from F. multiflora and inserted the FmSTS1 gene into the genome of Arabidopsis thaliana under the control of cauliflower mosaic virus 35S promoter to evaluate its biological functions. Southern blot and Northern blot analyses were performed to confirm the integration and expression of FmSTS1 in Arabidopsis. A new stilbene compound, which was identified as trans-piceid, was found to accumulate...
Effects of potassium chloride and nitric oxide on growth and physiological characteristics of winter wheat under salt stressSpecial Issue: Gasotransmitters
Y.J. DONG, Q. ZHANG, X.L. DAI, M.R. HE
Biologia plantarum 64: 258-265, 2020 | DOI: 10.32615/bp.2019.091
A hydroponic culture was conducted to evaluate the effects of KCl and sodium nitroprusside (SNP; a nitric oxide donor) in wheat seedlings under salt stress. Exposure to 100 mM NaCl for 7 d decreased biomass of wheat seedlings, root activity and H+-ATPase activity, significantly increased free proline content, reactive oxygen species (ROS) accumulation and lipid peroxidation, and suppressed the activity of superoxide dismutase (SOD). Moreover, NaCl stress significantly decreased the K+ and increased the Na+ content. Addition of KCl or SNP led to the increase in root activity and soluble protein content, stimulated the...
Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensisOriginal Papers
C. ZHANG, Q.-N. JIANG, Y.-G. WANG, J.-X. FU, B. DONG, L.-H. ZHOU, H.-B. ZHAO
Biologia plantarum 64: 253-257, 2020 | DOI: 10.32615/bp.2020.016
Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary. However, related works concerning reference gene selection have not been carried on many plant species, especially endangered ones. The aim of the present study was to select dependable reference genes for expression normalization of an endangered plant species with medicinal and ornamental value: Sinocalycanthus chinensis (Calycanthaceae). Nine reference genes with stable expressions were chosen for further analysis according to transcriptomic sequencing...
Do soil and leaf silicon content affect leaf functional traits in Deschampsia caespitosa from different habitats?Original Papers
M. GRAŠIČ, T. SAKOVIČ, D. ABRAM, K. VOGEL-MIKUŠ, A. GABERŠČIK
Biologia plantarum 64: 234-243, 2020 | DOI: 10.32615/bp.2019.155
The purpose of this study was to show the extent of phenotypic plasticity of the grass Deschampsia caespitosa from four habitats with different soil properties by comparing selected leaf traits and content of silicon and other elements. Morphological, biochemical, and optical properties were examined in leaves, but content of silicon and other elements also in soil samples. Plant-available silicon in the soil was determined following extraction in CaCl2. Bulk element analysis was conducted using X-ray fluorescence spectrometry. The habitats of D. caespitosa differed significantly according to soil structure, which resulted...
Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbataOriginal Papers
A. CHÁVEZ-CORTAZAR, M. MATA-ROSAS, K. OYAMA, M.S. SAMAIN, M. QUESADA
Biologia plantarum 64: 224-233, 2020 | DOI: 10.32615/bp.2020.006
The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The...
Comparative proteomics of phloem exudates reveals long-distance signals potentially involved in Litchi chinensis floweringBrief Communications
L.-J. HUANG, H.-B. CHEN, Z.-Q. HU, X.-Y. LU, H.-Y. WANG, H. LIU, B.-Y ZHOU
Biologia plantarum 64: 220-224, 2020 | DOI: 10.32615/bp.2019.163
It is well known that phloem is essential for transporting proteins as long-distance signals. In this study, a proteomic approach was carried out to identify proteins accumulated in phloem exudates at the pre-floral induction (PFId) stage, at the floral induction (FId) stage, and at the floral initiation (FIn) stage. As a result, 45 phloem exudate proteins were detected. Six proteins were found at all three stages. Sixteen proteins were specific to the PFId stage, 14 proteins were specific to the FId and FIn stages. From the enriched gene ontology (GO)-terms related to transport, signaling, hormone, and development, 12 transport-related, 5 signaling-related,...
Analysis of LEA protein family members in Lepidium apetalum seeds and the expression of LaLEA1 in seedlings in response to abiotic stressesOriginal Papers
Q.L. YANG, H. LU, Q. ZHOU, H.T. XIE, J.Y. LI, Z.Y. CHEN, S.C. HAN, H.P. ZHAO, H.X. ZHAO
Biologia plantarum 64: 211-219, 2020 | DOI: 10.32615/bp.2019.161
To study the biological function of late embryogenesis abundant (LEA) proteins in Lepidium apetalum Willd., genes encoding LEA family proteins were identified from the seed transcriptome. The sequence most closely related to germination at a low temperature was selected and gene expressions in response to low temperature stress further studied. The results show that 27 LEA genes were expressed in seeds germinating at the low temperature: 3 genes were upregulated, 20 were downregulated, and 4 were not significantly different from controls. The most prominent of the upregulated genes, LaLEA1, contained an open reading frame of 624...
Molecular characterization of the promoter of the stress-inducible ZmMYB30 gene in maizeOriginal Papers
J. LUO, C.M. YU, M. YAN, Y. H. CHEN
Biologia plantarum 64: 200-210, 2020 | DOI: 10.32615/bp.2020.011
The ZmMYB30 is a member of the myeloblastosis (MYB) transcription factor superfamily, which has been shown to be a transcription regulator in abiotic stress tolerance in maize (Zea mays). To further identify the biological function of ZmMYB30 and reveal how its expression is induced in response to stress, we isolated the ZmMYB30 promoter and conducted a functional analysis. A 1461-bp promoter fragment was cloned and sequenced. Motif prediction using PlantCARE revealed several stress-responsive elements in the promoter sequence. Deletions in the promoter sequence affected the activity of the promoter and demonstrated that...
Characterization and functional analysis of microRNA399 in Cunninghamia lanceolataOriginal Papers
F.R. ZHU, Z.B. QIU, Y.M. ZHANG, X. R. ZHANG, W. L.WANG
Biologia plantarum 64: 193-199, 2020 | DOI: 10.32615/bp.2020.037
The miR399 is a conserved microRNA (miRNA) family, and it has been characterized as an essential regulator of phosphorus transport in plants. However, the biological function of miR399 in Cunninghamia lanceolata is still largely unclear. In this study, the comparison of mature miR399 sequence revealed a high similarity between Arabidopsis thaliana and C. lanceolate, and the pre-miR399 was capable of forming a typical stem-loop hairpin structure. A gene PHOSPHATE 2 (PHO2) was identified as a target of cln-miR399 using 5' rapid amplification of cDNA ends. Furthermore, the relationship between cln-miR399...
Differences in physiological traits at the initial stage of Fusarium head blight infection in wheatOriginal Papers
V. SPANIC, Z. ZDUNIC, G. DREZNER, M. VILJEVAC VULETIC
Biologia plantarum 64: 185-192, 2020 | DOI: 10.32615/bp.2020.014
Wheat (Triticum aestivum L.) is leading cereal crop worldwide, but its yield is highly affected due to various diseases, especially Fusarium head blight (FHB), which affects the metabolism of plants. The present study was conducted at the Agricultural Institute Osijek using three winter wheat cultivars (Apache, Bezostaya1, and U1) during 2016/2017. The objectives of our studies were to examine differences in physiological characteristics of FHB resistance among wheat cultivars in the early stage of infection. The FHB incidence and severity was the highest in 'Bezostaya1'. Results suggest that activation of some anti-oxidative enzymes in the...
Calcium distribution during anther development in Impatiens balsaminaOriginal Papers
S.J. YANG, W.Y. LIANG, J. SHI, L. PENG, R. ZHENG
Biologia plantarum 64: 178-184, 2020 | DOI: 10.32615/bp.2019.147
The subcellular localization of loosely bound calcium in developing anthers of Impatiens balsamina (rose balzam) was investigated using potassium antimonate to precipitate the ions. At the sporogenous stage, few calcium precipitates were found in the young anthers. During meiosis of the microspore mother cell, the amount of calcium precipitates increased both inside these cells and in their cell walls. After meiosis, the resulting microspores accumulated abundant calcium precipitates in their cytoplasm and vacuoles. The microspores underwent mitosis to produce a bicellular pollen grain, in which the large vacuoles disintegrated, and the calcium...
Altered fatty acid composition of Nicotiana benthamiana and Nicotiana excelsior leaves under transient overexpression of the cyanobacterial desC geneOriginal Papers
M. BERESTOVOY, O.S. PAVLENKO, A.A. TYURIN, E.N. GORSHKOVA, I.V. GOLDENKOVA-PAVLOVA
Biologia plantarum 64: 167-177, 2020 | DOI: 10.32615/bp.2019.144
Transient heterologous gene expression in two model plant species, Nicotiana benthamiana and N. excelsior, has been used to study the localization of the heterologous Δ9 acyl-lipid desaturase (Δ9 desaturase) of Synechococcus vulcanus in different cell compartments and its functional activity in the cases of the cytosol, chloroplast, and endoplasmic reticulum (ER) localization. The functional activity and substrate specificity of the heterologous desaturase under the conditions of transient expression have been confirmed by comparison of fatty acid (FA) profiles. The Δ9 desaturase, responsible for the synthesis of...
Light and temperature receptors and their convergence in plantsOriginal Papers
J. SONG, W. WU, B. HU
Biologia plantarum 64: 159-166, 2020 | DOI: 10.32615/bp.2019.104
Light and temperature are two essential environmental cues for plants, helping to optimize plant body architecture and physiology. To sense a broad spectrum of sun radiation spanning from UV-B to far-red wavelength, plants are equipped with a sophisticated array of photoreceptors, including phytochromes, cryptochromes, phototropins, Zeitlupes, and UV-B photoreceptor UVR8. On the contrary, since the thermodynamic effects extensively affect the molecular and supramolecular structures, it is difficult to identify the entry point or initial receptor of temperature. Even so, several putative temperature sensors have been proposed, such as calcium ion channels,...
The enhancement of salt stress tolerance by salicylic acid pretreatment in Arabidopsis thalianaOriginal Papers
L.-L. YU, Y. LIU, F. ZHU, X.-X. GENG, Y. YANG, Z.-Q. HE, F. XU
Biologia plantarum 64: 150-158, 2020 | DOI: 10.32615/bp.2019.151
Salicylic acid (SA) is an important plant hormone involved in the activation of defense responses against environmental stresses. However, there are still large of unsolved mysteries about how SA pretreatment affects the establishment of plant stress tolerance. In this study, application of SA at different concentrations and different times were conducted to investigate their effects on the response of Arabidopsis seedlings to salt stress. The pretreatment with 10 or 20 μM SA for more than 6 h promoted Arabidopsis seedlings resistance to salt stress. On the other hand, pretreatment with 200 μM SA reduced Arabidopsis resistance...
Drought tolerance of transgenic rice overexpressing maize C4-PEPC gene related to increased anthocyanin synthesis regulated by sucrose and calciumOriginal Papers
Y.F. HE, Y.F. XIE, X. LI, J. YANG
Biologia plantarum 64: 136-149, 2020 | DOI: 10.32615/bp.2020.031
In order to reveal the role of sucrose (Suc) in early drought response in plants, transgenic rice (Oryza sativa L.) plants overexpressing the maize (Zea mays L.) C4-phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) gene (C4-pepc) (PC) and their untransformed wild type (WT) were used under 12 % (m/v) polyethylene glycol 6000 to simulate drought conditions. The results showed that PC has higher relative water content, the increased Suc content, and anthocyanin accumulation than WT during PEG treatment. By spraying 1 % Suc and 1 % Suc non-metabolic analog, turanose, on these plants, Suc...
Genome-wide transcriptome profiling provides new insights into bud dormancy in pearOriginal Papers
L.L. SUN, R. ZHANG, S.T. HE, J.P. LIU, G.Q. LIU
Biologia plantarum 64: 129-135, 2020 | DOI: 10.32615/bp.2019.116
Dormancy is important for the pear (Pyrus pyrifolia) to survive a harsh environment. The molecular base of dormancy in pear, especially in some local cultivars, is still unclear. Genome-wide transcriptome analysis in flower buds of cv. Huangli (an excellent local cultivar native to Guizhou mountain area in China) was conducted to explore the mechanism regulating bud dormancy in pear. For the release of endo-dormancy 223 chilling hours (CHs) was needed in Huangli flower buds, which was less than in commercial cultivars. Comparisons of transcript amounts among seven dates during dormancy (30 Oct. vs. 15 Nov., 15 Nov. vs. 30...
Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservationOriginal Papers
E. POKORNÁ, M. FALTUS, P. MÁCHOVÁ, J. ZÁMEČNÍK, M. FULÍN
Biologia plantarum 64: 119-128, 2020 | DOI: 10.32615/bp.2019.148
Cryostorage techniques have been developed to preserve the most valuable genotype from an endangered native population of Populus × canescens Aiton Sm. (grey poplar), which is located in the floodplain forest in the South Moravia region of the Czech Republic and which is difficult to propagate using cuttings. The prevailing genotype with valuable traits was selected by the simple sequence repeats method. This genotype was used to determine the most effective pre-cultivation conditions (cold hardening, and cold hardening combine with osmotic treatment) on dehydration tolerance and post-thaw recovery of the grey poplar shoot tips. The pre-cultivation...
Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense responseOriginal Papers
A. KUMARI, R. KAUR
Biologia plantarum 64: 110-118, 2020 | DOI: 10.32615/bp.2019.095
Di-n-butyl phthalate (DBP) is one of the frequently detected phthalates in environmental samples. The effects of phthalates are extensively studied in the animals but the effects on plants are scarce. Therefore, the present study is aimed to envisage the effects of DBP on the antioxidative defense system in Hordeum vulgare L. seedlings grown under laboratory conditions for 7 d. The activities of different antioxidative enzymes were enhanced in the shoots. In the roots, the activity of guaiacol peroxidase increased and the catalase activity decreased initially but increased at higher DBP concentrations, whereas the activities of superoxide...
High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in CucumisOriginal Papers
X. YU, Z. ZHU, T. ZHANG, J. LI, C. CHENG, Q. LOU, C.-O. OTTOSEN, J. CHEN
Biologia plantarum 64: 104-109, 2020 | DOI: 10.32615/bp.2019.121
Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis ×hytivus and its parents. The different developmental stages of leaves of C.×hytivus showing contrasting leaf colour were compared...
Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stressesOriginal Papers
Y.-M. ZHANG, H.-Q. WANG, D.-M. LIU, R.-J. LIU
Biologia plantarum 64: 95-103, 2020 | DOI: 10.32615/bp.2019.112
Late embryogenesis abundant (LEA) proteins are important for abiotic stress tolerance in diverse organisms. Within the LEA protein superfamily, group 4 members are characterized by a conserved N-terminal region and a structurally disordered C-terminal region that varies regarding length and amino acid content. Previous in vitro assays have suggested that the conserved N-terminal region shared by group 4 LEA proteins is critical for forming an amphipathic α-helix and protecting enzymatic activities from the adverse effects of desiccation or freezing. However, the cellular roles of the varying C-terminal region remain largely to be characterized....
Reactive oxygen species derived from NADPH oxidase regulate autophagyOriginal Papers
H. JING, Z. WANG, L. ZHANG, G. ZHOU, Z. GU, Y. SHAN, Y. ZHOU, Z. YANG, Z. KONG
Biologia plantarum 64: 87-94, 2020 | DOI: 10.32615/bp.2019.127
Reactive oxygen species (ROS) originating from nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) play vital roles in regulating autophagy. However, the relationship between autophagy and NOX in common wheat (Triticum aestivum L.) is still unknown. In order to clarify the mechanism of autophagy in wheat, ROS content, NOX activity, and autophagy levels in root tip cells under condition of N or C deficiency were measured. The results showed that the N and C deficiency increased the production of superoxide anions (O2-.) and hydrogen peroxide (H2O2) via the NOX activation, leading...