Photosynthetica, 2015 (vol. 53), issue 2

Photosynthetica 2015, 53(2):223-230 | DOI: 10.1007/s11099-015-0096-3

Differential response of photosynthetic apparatus of cyanobacterium Nostoc muscorum to Pb and Cd toxicity

S. Dixit1, D. P. Singh1,*
1 Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow, India

Nostoc muscorum cells showed metal-induced decrease in the relative growth, pigment contents, O2 evolution, and Hill activity in response to lead (Pb2+) and cadmium (Cd2+) treatment, which was further accentuated with increase in metal exposure time and metal concentration. I50 concentrations (50% growth inhibitory concentrations) of Pb2+ and Cd2+ for growth of N. muscorum were 55 and 21 μg mL-1, respectively. These results indicated that the cells of N. muscorum were more susceptible to Cd2+ in comparison to Pb2+. The O2 production was relatively more sensitive to both heavy metals (I50: 16 and 10 μg mL-1 of Pb2+ and Cd2+, respectively) than the Hill activity (I50: 61 and 39 μg mL-1 of Pb2+ and Cd2+, respectively). Further, measurement of Hill activity in the presence of metals and electron donors showed that inhibition sites of both Pb2+ and Cd2+ were located on the oxidizing site of PSII. The chlorophyll a (Chl a) and phycobilisome (PB) fluorescence emission spectra showed that energy transfer from Chl a and PB to PSII reaction center was more susceptible to Cd2+ than Pb2+.

Keywords: chlorophyll fluorescence; electrons donors; heavy metals; Hill activity; oxygen evolution

Received: April 25, 2013; Accepted: May 30, 2014; Published: June 1, 2015Show citation

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Dixit, S., & Singh, D.P. (2015). Differential response of photosynthetic apparatus of cyanobacterium Nostoc muscorum to Pb and Cd toxicity. Photosynthetica, 53(2), 223-230. doi: 10.1007/s11099-015-0096-3.
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