Photosynthetica, 2012 (vol. 50), issue 4

Photosynthetica 2012, 50(4):577-586 | DOI: 10.1007/s11099-012-0061-3

Nitrogen enhanced photosynthesis of Miscanthus by increasing stomatal conductance and phosphoenolpyruvate carboxylase concentration

X. P. Feng1, Y. Chen1, Y. H. Qi1, C. L. Yu1, Bing-Song Zheng2,*, M. Brancourt-Hulmel3, De-An Jiang1,*
1 State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China
2 School of Forestry & Biotechnology, Zhejiang A & F University, Linan, China
3 INRA/USTL UMR SADV 1281, Estrées-Mons, Péronne Cedex, France

Miscanthus is one of the most promising bioenergy crops with high photosynthetic nitrogen-use efficiency (PNUE). It is unclear how nitrogen (N) influences the photosynthesis in Miscanthus. Among three Miscanthus genotypes, the net photosynthetic rate (P N) under the different light intensity and CO2 concentration was measured at three levels of N: 0, 100, and 200 kg ha-1. The concentrations of chlorophyll, soluble protein, phosphoenolpyruvate carboxylase (PEPC), ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit, leaf anatomy and carbon isotope discrimination (Δ) in the leaf were analyzed to probe the response of photosynthesis in Miscanthus genotypes to N levels. P N in all genotypes rose significantly as N application increased. The initial slope of response curves of P N to C i was promoted by N application in all genotypes. Both stomatal conductance and C i were increased with increased N supply, indicating that stomatal factors played an important role in increasing P N. At a given C i, P N in all genotypes was enhanced by N, implying that nonstomatal factors might also play an important role in increasing P N. Miscanthus markedly regulated N investment into PEPC rather than the Rubisco large subunit under higher N conditions. Bundle sheath leakiness of CO2 was constant at about 0.35 for all N levels. Therefore, N enhanced the photosynthesis of Miscanthus mainly by increasing stomatal conductance and PEPC concentration.

Keywords: bundle sheath leakiness of CO2; leaf mass per unit area; nitrogen-use efficiency; Rubisco

Received: April 1, 2012; Accepted: July 19, 2012; Published: December 1, 2012Show citation

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Feng, X.P., Chen, Y., Qi, Y.H., Yu, C.L., Zheng, B., Brancourt-Hulmel, M., & Jiang, D. (2012). Nitrogen enhanced photosynthesis of Miscanthus by increasing stomatal conductance and phosphoenolpyruvate carboxylase concentration. Photosynthetica, 50(4), 577-586. doi: 10.1007/s11099-012-0061-3.
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    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Clifton-Brown, J., Robson, P., Allison, G. et al.: Miscanthus: breeding our way to a better future. - Aspects Appl. Biol. 90: 199-206, 2008.
    3. Cosentino, S.L., Patane, C., Sanzone, E., Copani, V., Foti, S.: Effects of soil water content and nitrogen supply on the productivity of Miscanthus × giganteus Greef et Deu. in a Mediterranean environment. - Ind. Crop Prod. 25: 75-88, 2007. Go to original source...
    4. Cousins, A.B., Badger, M.R., von Caemmerer, S.: Carbonic anhydrase and its influence on carbon isotope discrimination during C4 photosynthesis. Insights from antisense RNA in Flaveria bidentis. - Plant Physiol. 141: 232-242, 2006. Go to original source...
    5. Dohleman, F.G., Heaton, E.A., Leakey, A.D.B., Long, S.P.: Does greater leaf-level photosynthesis explain the larger solar energy conversion efficiency of Miscanthus relative to switchgrass? - Plant Cell Environ. 32: 1525-1537, 2009. Go to original source...
    6. Ercoli, L., Mariotti, M., Masoni, A., Bonari, E.: Effect of irrigation and nitrogen fertilization on biomass yield and efficiency of energy use in crop production of Miscanthus. - Field Crops Res. 63: 3-11, 1999. Go to original source...
    7. Ethier, G.J., Livingston, N.J., Harrison, D.L., Black, T.A., Moran, J.A.: Low stomatal and internal conductance to CO2 versus Rubisco deactivation as determinants of the photosynthetic decline of ageing evergreen leaves. - Plant Cell Environ. 29: 2168-2184, 2006. Go to original source...
    8. Farquhar, G.D.: On the nature of carbon isotope discrimination in C4 species, - Aust. J. Plant Physiol. 10: 205-226, 1983. Go to original source...
    9. Ghannoum, O., Evans, J.R., Chow, W.S., Andrews, T.J., Conroy, J.P., von Caemmerer, S.: Faster rubisco is the key to superior nitrogen-use efficiency in NADP-malic enzyme relative to NAD-malic enzyme C4 grasses. - Plant Physiol. 137: 638-650, 2005. Go to original source...
    10. Ghannoum, O., von Caemmerer, S., Ziska, L.H., Conroy, J.P.: The growth response of C4 plants to rising atmospheric CO2 partial pressure: a reassessment. - Plant Cell Environ. 23: 931-942, 2000. Go to original source...
    11. Greef, J.M., Deuter, M.: Syntaxonomy of Miscanthus × giganteus Greef et Deu. - Angew. Bot. 67: 87-90, 1993.
    12. Green, T.H., Mitchell, R.J.: Effects of nitrogen on the response of loblolly pine to water stress. I. Photosynthesis and stomatal conducance. - New Phytol. 122: 627-633, 1992.
    13. Hatch, M.D., Agostino, A., Jenkins, C.L.D.: Measurement of the leakage of CO2 from bundlesheath cells of leaves during C4 photosynthesis. - Plant Physiol. 108: 173-181, 1995. Go to original source...
    14. Henderson, S.A., von Caemmerer, S., Farquhar, G.D.: Short term measurements of carbon isotope discrimination in several C4 species. - Aust. J. Plant Physiol. 19: 263-285, 1992. Go to original source...
    15. Kromdijk, J., Schepers, H.E., Albanito, F. et al.: Bundle sheath leakiness and light limitation during C4 leaf and canopy CO2 uptake. - Plant Physiol. 148: 2144-2155, 2008. Go to original source...
    16. Kubásek, J., Šetlík, J., Dwyer, S., Šantrůček, J.: Light and growth temperature alter carbon isotope discrimination and estimated bundle sheath leakiness in C4 grasses and dicots. - Photosynth. Res. 91: 47-58, 2007. Go to original source...
    17. Le Quéré, C., Raupach, M.R., Canadell, J.G. et al.: Trends in the sources and sinks of carbon dioxide. - Nature Geosci. 2: 831-836, 2009. Go to original source...
    18. Leegood, R.C., von Caemmerer, S.: Some relationships between contents of photosynthetic intermediates and the rate of photosynthetic carbon assimilation in leaves of Zea mays L. - Planta 178: 258-266,1989. Go to original source...
    19. Makino, A., Sakuma, H., Sudo, E., Mae, T.: Differences between maize and rice in N-use efficiency for photosynthesis and protein allocation. - Plant Cell Physiol. 44: 952-956, 2003. Go to original source...
    20. Maranville, J.W., Madhavan, S.: Physiological adaptations for nitrogen use efficiency in sorghum. - Plant Soil 245: 25-34, 2002. Go to original source...
    21. McKendry, P.: Energy production from biomass (part 1): overview of biomass. - Bioresour. Technol. 83: 37-46, 2002. Go to original source...
    22. Meinzer, F.C., Zhu, J.: Nitrogen stress reduces the efficiency of the C4 CO2 concentrating system, and therefore quantum yield, in Saccharum (sugarcane) species. - J. Exp. Bot. 49: 1227-1234,1998. Go to original source...
    23. Pengelly, J.J.L., Sirault, X.R.R., Tazoe, Y., Evans, J.R., Furbank, R.T., von, Caemmerer, S.: Growth of the C4 dicot Flaveria bidentis: photosynthetic acclimation to low light through shifts in leaf anatomy and biochemistry. - J. Exp. Bot. 61: 4109-4122, 2010. Go to original source...
    24. Porra, R.J., Thompson, W.A., Kriedemann, P.E.: Determination of accurate extinction coefficients and simultaneous-equations for assaying chlorophyll-A and chlorophyll-B with 4 different solvents-verification of the concentration of chlorophyll standards by atomic-absorption spectroscopy. - Biochim. Biophys. Acta 975: 384-394, 1989. Go to original source...
    25. Ranjith, S.A., Meinzer, F.C., Perry, M.H., Thom, M.: Partitioning of carboxylase activity in nitrogen stressed sugarcane and its relationship to bundle sheath leakiness to CO2, photosynthesis and carbon isotope discrimination. - J. Plant Physiol. 22: 903-911, 1995. Go to original source...
    26. Remlein-Starosta, D.: Diseases of bioenergy crops. - Prog. Plant Protect. 47: 351-357, 2007.
    27. Sage, R.F.: The evolution of C4 photosynthesis. - New Phytol. 161: 341-370, 2004. Go to original source...
    28. Still, C.J., Berry, J.A., Collatz, G.J., DeFries, R.S.: Global distribution of C3 and C4 vegetation: Carbon cycle implications. - Global Biogeochem. Cycles 17: 1-14, 2003. Go to original source...
    29. Stuart, N.W.: Adaptation of the micro-Kjeldahl method for the determination of nitrogen in plant tissues. - Plant Physiol. 11: 173-179, 1936. Go to original source...
    30. Sugiyama, T., Mizuno, M., Hayashi, M.: Partitioning of nitrogen among ribulose-1,5-bisphosphate carboxylase oxygenase, phosphoenolpyruvate carboxylase, and pyruvate orthophosphate dikinase as related to biomass productivity in maize seedlings. - Plant Physiol. 75: 665-669, 1984. Go to original source...
    31. Tazoe, Y., Hanba, Y.T., Furumoto, T., Noguchi, K., Terashima, I.: Relationships between quantum yield for CO2 assimilation, activity of key enzymes and CO2 leakiness in Amaranthus cruentus, a C4 dicot, grown in high or low light. - Plant Cell Physiol. 49: 19-29, 2008. Go to original source...
    32. Tazoe, Y., Noguchi, K., Terashima, I.: Effects of growth light and nitrogen nutrition on the organization of the photosynthetic apparatus in leaves of a C4 plant, Amaranthus cruentus. - Plant Cell Environ. 29: 691-700, 2006. Go to original source...
    33. Uribelarrea, M., Crafts-Brandner, S.J., Below, F.E.: Physiological N response of field-grown maize hybrids (Zea mays L.) with divergent yield potential and grain protein concentration. - Plant Soil 316: 151-160, 2009. Go to original source...
    34. von Caemmerer, S., Millgate, A., Farquhar, G.D., Furbank, R.T.: Reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase by antisense RNA in the C4 plant Flaveria bidentis leads to reduced assimilation rates and increased carbon isotope discrimination. - Plant Physiol. 113: 469-477, 1997. Go to original source...
    35. Wang, D., Lu, Q., Li, X.F., Jiang, Q.S., Wu, J.X., Jiang, D.A.: Relationship between Rubisco activase isoform levels and photosynthetic rate in different leaf positions of rice plant. - Photosynthetica 47: 621-629, 2009. Go to original source...
    36. Wang, D.F., Portis, A.R., Moose, S.P., Long, S.P.: Cool C4 photosynthesis: Pyruvate Pi dikinase expression and activity corresponds to the exceptional cold tolerance of carbon assimilation in Miscanthus × giganteus. - Plant Physiol. 148: 557-567, 2008. Go to original source...
    37. Weng, J.H., Hsu, F.H.: Gas exchange and epidermal characteristics of Miscanthus populations in Taiwan varying with habitats and nitrogen application. - Photosynthetica 39: 35-41, 2001. Go to original source...
    38. Zhu, J., Meinzer, F.C.: Efficiency of C4 photosynthesis in Atriplex lentiformis under salinity stress. - Aust. J. Plant Physiol. 26: 79-86, 1999. Go to original source...

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