Photosynthetica, 2012 (vol. 50), issue 4

Photosynthetica 2012, 50(4):602-612 | DOI: 10.1007/s11099-012-0072-0

Ascorbate plays a key role in alleviating low temperature-induced oxidative stress in Arabidopsis

L. Y. Wang1,2, Q. Y. Zhang1, F. Wang1, X. Meng1, Q. W. Meng1,*
1 College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
2 Department of Biology, Dezhou University, Dezhou, Shandong, P.R. China

Low temperature has a negative impact on plant cells and results in the generation of reactive oxygen species (ROS). In order to study the role of ascorbate under chilling stress, the response of an ascorbate-deficient Arabidopsis thaliana mutant vtc2-1 to low temperature (2°C) was investigated. After chilling stress, vtc2-1 mutants exhibited oxidative damage. An increase in the H2O2 generation and the production of thiobarbituric acid reactive substances (TBARS), and a decrease in chlorophyll content, the maximal photochemical efficiency of PSII (Fv/Fm) and oxidizable P700 were also noted. The ratio of ascorbate/dehydroascorbate and reduced glutathione/oxidzed glutathione in the vtc2-1 mutants were reduced, compared with the wild type (WT) plants. The activities of antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), and soluble antioxidants were lower in the vtc2-1 mutants than those in WT plants. These results suggested that the ascorbate-deficient mutant vtc2-1 was more sensitive to chilling treatment than WT plants. The low temperature-induced oxidative stress was the major cause of the decrease of PSII and PSI function in the vtc2-1 mutants. Ascorbate plays a critical role of defense without which the rest of the ROS defense network is unable to react effectively.

Keywords: Arabidopsis thaliana; ascorbate; chilling stress; photoinhibition; reactive oxygen species

Received: October 2, 2011; Accepted: August 20, 2012; Published: December 1, 2012Show citation

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Wang, L.Y., Zhang, Q.Y., Wang, F., Meng, X., & Meng, Q.W. (2012). Ascorbate plays a key role in alleviating low temperature-induced oxidative stress in Arabidopsis. Photosynthetica, 50(4), 602-612. doi: 10.1007/s11099-012-0072-0.
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