Tuning of Oxidation Potential of Ferrocene for Ratiometric Redox Labelling and Coding of Nucleotides and DNA

Abstrakt

Cell sheet engineering has been progressing rapidly and shown promise in many cell based therapy. In this study, carbon quantum dots (CQDs) are incorporated as a sensitizer into TiO2 films to regulate the light-induced cell detachment performance. Depending on the amount of CQDs incorporated, nanodots or porous films are obtained. It is found properCQDs incorporation significantly improves the cell sheet detachment efficiency, and an intact cell sheet could be obtained after merely 5 min UV365 illumination, which is much faster than that from CQDs-free TiO2 film. Furthermore, cell sheets on CQDs sensitized TiO2 films show improved viability, alkaline phosphatase and collagen-I activity either before or after light-induced detachment. The improved cell detachment performance is ascribed to the effective inhibition of CQDs incorporation on charge recombination of TiO2 film and the microstructure variations, which increases the negative surface potential and changes the secondary structure of adsorbed extracellular protein molecules, and consequently results in faster