biologia plantarum

Biologia plantarum 55:641, 2011 | DOI: 10.1007/s10535-011-0162-z

Early stages of leaf development in has mutant of Arabidopsis thaliana

D. Janošević^1,*, B. Uzelac², D. Stojičić³, Lj. Tubić², S. Budimir²

¹ Institute of Botany and Botanical Garden "Jevremovac", Faculty of Biology, University of Belgrade, Belgrade, Serbia

² Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia

³ Faculty of Science, University of Niš, Niš, Serbia

The elucidation of molecular mechanisms underlying the leaf development can be facilitated by the detailed anatomical study of leaf development mutants. We present an analysis of leaf anatomy and morphogenesis during early developmental stages in has mutant of Arabidopsis thaliana. The recessive has mutation affects a number of aspects in plant development, including the shape and size of both cotyledons and leaves. The earliest developmental observations suggest almost synchronous growth of the first two leaf primordia of has mutant. No significant disruption of the cell division pattern in the internal tissue is observed at the earliest stages of development, with the major anatomical difference compared to wild type primordia being the untimely maturation of mesophyll tissue cells in has mutant. At the stage of leaf blade formation, structure disruption becomes clearly evident, by irregular arrangement of the cell layers and the lack of polarity in juvenile has leaves. One distinguishing feature of the mutant leaf anatomy is the absence of mesophyll tissue differentiation. Altered has mutant leaf morphology could be at least partially accounted for by the ectopic STM activity that was found at the base of leaf primordia during early stages of leaf development in has plants.

Keywords: in situ hybridization; insertional mutagenesis; leaf morphogenesis

Received: April 19, 2010; Accepted: June 21, 2010; Published: December 1, 2011Show citation