biologia plantarum

Biologia plantarum 55:696, 2011 | DOI: 10.1007/s10535-011-0170-z

Molecular characterization of a gene induced during wheat hypersensitive reaction to stripe rust

G. Zhang¹, Y. -M. Li¹, Y. -F. Sun¹, J. -M. Wang¹, B. Liu¹, J. Zhao¹, J. Guo¹, L. -L. Huang¹, X. -M. Chen², Z. -S. Kang^1,*

¹ State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, P. R. China

² USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, USA

A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2. The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively. Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein. Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.

Keywords: cell death; cloning; defense; expression profile; Puccinia striiformis; resistance; Triticum aestivum

Received: January 22, 2010; Accepted: May 3, 2010; Published: December 1, 2011Show citation