Biologia plantarum 43:563-569, 2000 | DOI: 10.1023/A:1002877917537

Pigment Diverse Mutants of Pseudomonas sp.: Inhibition of Fungal Growth and Stimulation of Growth of Cicer arietinum

A.K. Goel1, S.S. Sindhu1, K.R. Dadarwal1
1 Department of Microbiology, Chaudhary Charan Singh Haryana Agricultural University, Hisar -, India

A Pseudomonas strain MRS16 inhibited growth of different pathogenic fungi (Aspergillus sp., Fusarium oxysporum, Pythium aphanidermatum and Rhizoctonia solani) in vitro. Larger inhibition zones were obtained on nutrient agar and King's B media compared to potato dextrose agar and pigment production media. Mutants altered in production of fluorescent pigment were derived by nitrosoguanidine mutagenesis. The pigment overproducer mutant MRS16M-1 was more inhibitory whereas nonproducer mutant MRS16M-5 was less inhibitory than parent strain on nutrient agar medium. Addition of iron (100 µM FeCl3) in the medium decreased inhibition of fungal growth, suggesting the involvement of siderophores and other antifungal secondary metabolites. Seed bacterization of two cultivars of chickpea (Cicer arietinum cvs. H8618 and C235) differing in susceptibility to wilt caused initial root and shoot stunting at 5 d of growth followed by proliferation of secondary root growth at 10 d. Coinoculation of chickpea with Pseudomonas strain MRS16 or mutants and Rhizobium sp. Cicer strain Ca181 enhanced nodulation, nitrogen fixation and plant dry mass as compared to single inoculation with Rhizobium strain under sterile conditions.

Keywords: antifungal activity; chickpea; fluorescent pigment; nodulation; seed bacterization
Subjects: antifungal activity, Pseudomonas; Aspergilus sp.; chickpea, growth stimulation by Pseudomonas; Cicer arietinum; fluorescent pigment; fungi, growth inhibition by Pseudomonas; Fusarium oxysporum; growth stimulation by Pseudomonas, chickpea; nodulation, seed bacterization; pigment mutants of Pseudomonas, growth; Pseudomonas, pigment mutants; Pythium aphanidermatum; Rhizobium sp. Cicer strain; Rhizoctonia solani; seed bacterization, nodulation

Published: December 1, 2000Show citation

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Goel, A.K., Sindhu, S.S., & Dadarwal, K.R. (2000). Pigment Diverse Mutants of Pseudomonas sp.: Inhibition of Fungal Growth and Stimulation of Growth of Cicer arietinum. Biologia plantarum, 43(4), 563-569. doi: 10.1023/A:1002877917537.
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