Biologia plantarum 44:41-46, 2001 | DOI: 10.1023/A:1017962102950

Activities of Antioxidant Enzymes during Senescence of Prunus Armeniaca Leaves

F. Scebba1, L. Sebastiani1, C. Vitagliano1
1 Scuola Superiore di Studi Universitari e Perfezionamento "S. Anna", Pisa, Italy

During the period of senescence of apricot leaves changes in photosynthetic pigment contents and in the activities of some antioxidant enzymes (superoxide dismutase, catalase, peroxidase and ascorbate peroxidase) were analysed. Significant changes in pigment contents were, in most cases, correlated with changes in activities of the antioxidant enzymes. Modifications in superoxide dismutase and catalase isoform patterns were also observed during the progression of senescence. Both enzyme activities and isoenzyme patterns proved to be genotype-dependent.

Keywords: activated oxygen species; apricot; ascorbate peroxidase; catalase; peroxidase; superoxide dismutase
Subjects: antioxidant enzymes during leaf senescence; apricot; ascorbate peroxidase during leaf senescence; carotenoids as antioxidants during leaf senescence; catalase during leaf senescence; chlorophylls, leaf ontogeny; peroxidases during leaf senescence; Prunus armeniaca; senescence, antioxidant enzymes; superoxide dismutase during leaf senescence

Published: March 1, 2001Show citation

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Scebba, F., Sebastiani, L., & Vitagliano, C. (2001). Activities of Antioxidant Enzymes during Senescence of Prunus Armeniaca Leaves. Biologia plantarum, 44(1), 41-46. doi: 10.1023/A:1017962102950.
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    References

    1. Aebi, H.: Catalase in vitro.-Methods Enzymol. 105: 121-126, 1984. Go to original source...
    2. Beauchamp, C.O., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gel.-Anal. Biochem. 44: 276-287, 1971. Go to original source...
    3. Buchanan-Wollaston, V.: The molecular biology of leaf senescence.-J. exp. Bot. 48: 181-199, 1997. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantitation of micrograms quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Brewbacker, J.L., Upadhya, M.D., Makinen, J., MacDonald, T.: Isoenzyme polymorphism in flowering plants III. Gel electrophoretic methods and applications.-Physiol. Plant. 21: 930-949, 1968. Go to original source...
    6. Chen, G.X., Asada, K.: Ascorbate peroxidase in tea leaves: occurrence of two isoforms and the differences in their enzymatic and molecular properties.-Plant Cell Physiol. 30: 987-998, 1989.
    7. Del Rio, L.A., Pastori, G.M., Palma, J.M., Sandalio, L.M., Sevilla, F., Corpas, F.J., Jimenez, A., Lopez-Huertas, E., Hernandez, J. A.: The activated oxygen role of peroxisomes in senescence.-Plant Physiol. 116: 1195-1200, 1998. Go to original source...
    8. Goldstein, I.M., Weissmann, G.: Effects of generation of superoxide anion on permeability of liposomes.-Biochem. biophys. Res. Commun. 70: 452-458, 1977. Go to original source...
    9. Halliwell, B., Gutteridge, J.M.C.: Free Radicals in Biology and Medicine.-Oxford University Press, Oxford 1989.
    10. John, I., Hackett, R., Cooper, W., Drake, R., Farrel, A., Grierson, D.: Cloning and characterisation of tomato leaf senescence-related cDNAs.-Plant mol. Biol. 33: 641-651, 1997. Go to original source...
    11. Kleber-Janke, T., Krupinska, K.: Isolation of cDNA clones for genes showing enhanced expression in barley leaves during dark-induced senescence as well as during senescence under field conditions.-Planta 203: 332-340, 1997. Go to original source...
    12. Leopold, A.C.: Aging, senescence and turnover in plants.-BioScience 25: 659-662, 1975. Go to original source...
    13. Leshem, Y.Y.: Plant senescence processes and free radicals.-Free Radical Biol. Med. 5: 39-49, 1988.
    14. Lichthenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes.-Methods Enzymol. 148: 350-383, 1987. Go to original source...
    15. Madamanchi, N.R., Donahue, J.L., Cramer, C.L., Alscher, R.G., Pedersen, K.: Differential response of Cu,Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulphur dioxide.-Plant mol. Biol. 26: 95-103, 1994. Go to original source...
    16. Maehly, A.C., Chance, B.: The assay of catalase and peroxidase.-In: Glick, D. (ed.): Methods of Biochemical Analysis. Vol. 1. P. 357. Interscience Publishers, New York 1954. Go to original source...
    17. McRae, D.G., Thompson, J.E.: Senescence-dependent changes in superoxide anion production by illuminated chloroplasts from bean leaves.-Planta 158: 185-193, 1983. Go to original source...
    18. Noodén, L.D., Leopold, A.C.: Phytohormones and the endogenous regulation of senescence and abscission.-In: Letham D.S., Goodwin, P.B., Higgins, T.J.V. (ed.): Phytohormones and Related Compounds: A Comprehensive Treatise. Vol. 2. Pp. 329-369. Elsevier, Amsterdam 1978.
    19. Panavas, T., Pikula, A., Reid, P.D., Rubinstein, B., Walker, E.L.: Identification of senescence-induced genes from daylily petals.-Plant mol. Biol. 40: 237-248, 1999. Go to original source...
    20. Rao, M.V., Paliyath, G., Ormrod, D.P.: Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.-Plant Physiol. 110: 125-136, 1996. Go to original source...
    21. Scandalios, J.G.: Oxidative Stress and the Molecular Biology of Antioxidant Defenses.-Cold Spring Harbor Laboratory Press, New York 1997.
    22. Thimann, K.V.: The senescence of leaves.-In: Thimann, K.V. (ed.): Senescence in Plants. Pp. 85-115. RCR Press, Boca Raton 1986.
    23. Thompson, J.E.: The molecular bases for membrane deterioration during senescence.-In: Noodén, L.D., Leopold, A.C. (ed.): Senescence and Aging in Plants. Pp. 51-83. Academic Press, San Diego 1988. Go to original source...
    24. Thompson, J.E., Ledge, R.L., Barber, R.F.: The role of free radicals in senescence and wounding.-New Phytol. 105: 317-344, 1987. Go to original source...
    25. Woodbury, W., Spencer, A.K., Sthamann, M.A.: An improved procedure using ferricyanide for detecting catalase isozymes.-Anal. Biochem. 44: 301-305, 1971. Go to original source...

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