Biologia plantarum 48:13-18, 2004 | DOI: 10.1023/B:BIOP.0000024269.72171.42

Rapid in vitro Regeneration of Sesbania drummondii

S.B. Cheepala1, N.C. Sharma1, S.V. Sahi1,*
1 Biotechnology Center, Department of Biology, Western Kentucky University, Bowling Green, USA

This paper describes rapid propagation of Sesbania drummondii using nodal explants isolated from seedlings and young plants. The nodal segments proliferated into multiple shoots on Murashige and Skoog's (MS) medium supplemented with 22.2 μM benzyladenine. MS medium containing 2.2 and 4.5 μM thidiazuron induced 5 - 6 shoots per stem node from 3-month-old plants. Nodal explants when cultured on MS medium containing combinations of benzyladenine (8.8 and 11.1 μM) and indole-3-butyric acid (0.24 - 2.46 μM) or indole-3-acetic acid (0.28 - 2.85 μM) gave lesser number of shoots. Callus induced on cotyledonary explants when subcultured on 2.2 μM thidiazuron containing medium resulted in its mass proliferation having numerous embryoid-like structures. Indole-3-butyric acid (0.24 - 2.46 μM) was found suitable for root induction. In vitro regenerated plants were acclimatized in greenhouse conditions.

Keywords: 6-benzyladenine; indole-3-acetic acid; indole-3-butyric acid; medicinal plant; metal hyperaccumulator; micropropagation; thidiazuron
Subjects: auxins; cytokinins; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; in vitro culture, root, rooting; medicinal plants; metal hyperaccumulator; nutrient medium, Murashige and Skoog; Sesbania drummondii; thidiazuron

Published: March 1, 2004Show citation

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Cheepala, S.B., Sharma, N.C., & Sahi, S.V. (2004). Rapid in vitro Regeneration of Sesbania drummondii. Biologia plantarum, 48(1), 13-18. doi: 10.1023/B:BIOP.0000024269.72171.42.
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