Biologia plantarum 49:463-465, 2005 | DOI: 10.1007/s10535-005-0030-9

Production of saponins from Panax ginseng suspension and adventitious root cultures

L. Langhansova1, P. Marsik1, T. Vanek1,*
1 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic

Biomass growth and ginsenoside production in cell suspension and adventitious roots of Panax ginseng C.A. Meyer cultures cultivated both in Erlenmayer flasks and a 3 dm3 bioreactor were studied. The maximum content of ginsenosides was found in the suspension culture cultivated in the bioreactor (4.34 % dry mass), however the saponin content was limited to two major ginsenosides, Rb1 and Rg1. The production of ginsenosides in adventitious roots was lower (1.45 or 1.72 % dry mass), nevertheless, the full range of ginsenosides was detected.

Keywords: callus; suspension cultures; bioreactor; ginsenosides
Subjects: adventitious shoot, root; auxins; bioreactor; ginsenosides; in vitro culture, adventitious shoots and roots; medicinal plants; nutrient medium MS (Murashige and Skoog); nutrient medium SH (Schenk and Hildebrandt); Panax ginseng; saponins

Received: September 10, 2004; Accepted: March 3, 2005; Published: September 1, 2005Show citation

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Langhansova, L., Marsik, P., & Vanek, T. (2005). Production of saponins from Panax ginseng suspension and adventitious root cultures. Biologia plantarum, 49(3), 463-465. doi: 10.1007/s10535-005-0030-9.
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