biologia plantarum

Biologia plantarum 49:637-639, 2005 | DOI: 10.1007/s10535-005-0066-x

High frequency in vitro regeneration of Lathyrus sativus L.

D. P. Barik¹, U. Mohapatra¹, P. K. Chand^1,*

¹ Plant Cell, Tissue and Organ Culture Facility, Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Orissa, India

A simple and efficient protocol for high frequency plant regeneration of a grain legume grasspea (Lathyrus sativus L.) is described. Of different explant types tested epicotyl segments were most responsive. Murashige and Skoog's (1962) medium augmented with 17.76 µM 6-benzyladenine + 10.74 µM α-naphthaleneacetic acid showed the highest percentage of direct shoot regeneration. Among cultivars IC-120487 showed the highest regeneration frequency (80 %) with maximum shoot numbers (8.2 shoots per explant) and maximum average shoot length (4.1 cm). About 78 % of the regenerated shoots were rooted in half-strength MS medium containing 2.85 µM indole-3-acetic acid. After primary hardening the plantlets were established in soil with a survival rate of 75 %.

Keywords: adventitious regeneration; epicotyl segment; grain legume; grasspea

Subjects: Agrobacterium tumefaciens; auxins; cytokinins; grasspea, high frequency regeneration in vitro; in vitro culture, regeneration, organogenesis, morphogenesis; Lathyrus sativus; nutrient medium MS (Murashige and Skoog)

Received: March 23, 2004; Accepted: February 23, 2005; Published: December 1, 2005Show citation