Biologia plantarum 50:501-506, 2006 | DOI: 10.1007/s10535-006-0079-0

In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd

M. S. Rao1, S. D. Purohit1,*
1 Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, India

Multiple shoot buds could be induced directly from internode explants of Celastrus paniculatus inoculated on Murashige and Skoog's (MS) medium containing different growth regulators. The best response was obtained when 4.44 μM 6-benzylaminopurine (BAP) was incorporated in the medium. Incorporation of indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) did not improve response, rather promoted callusing. Adventitious shoot buds could be multiplied and elongated on MS medium containing 2.22 μM BAP. Rooting of shoots (80 %) was obtained when their bases were dipped in pre-autoclaved indole-3-butyric acid (IBA) solution (2.45 mM) for 10 min followed by their implantation on medium containing 1/4 MS salts, 1.0 % sucrose and 0.6 % agar. Out of 500 plantlets subjected to hardening, 410 were successfully hardened under greenhouse conditions. Twenty plants were established in field while remaining of them were transferred to nursery conditions without any mortality.

Keywords: adventitious shoot buds; auxins; cytokinins; internode segments; medicinal plant
Subjects: acclimatization, acclimation; auxins; Celastrus paniculatus; cytokinins; ex vitro transfer; hardening; in vitro culture, regeneration, proliferation, differentiation; medicinal plants; nutrient medium, Murashige and Skoog

Received: September 27, 2004; Accepted: September 15, 2005; Published: December 1, 2006Show citation

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Rao, M.S., & Purohit, S.D. (2006). In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd. Biologia plantarum, 50(4), 501-506. doi: 10.1007/s10535-006-0079-0.
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