Biologia plantarum 52:681-686, 2008 | DOI: 10.1007/s10535-008-0131-3

Ascorbate and glutathione metabolism in embryo axes and cotyledons of germinating lupine seeds

M. Garnczarska1,*, Ł. Wojtyla1
1 Department of Plant Physiology, A. Mickiewicz University, Poznań, Poland

Changes in ascorbate and glutathione contents and the activities and isoenzyme patterns of enzymes of the ascorbate-glutathione cycle were investigated in embryo axes and cotyledons of germinating lupine (Lupinus luteus L.) seeds. Ascorbate content was not significantly affected over the initial 12 h of imbibition in embryo axes, but afterwards increased, with the most rapid accumulation coinciding with radicle emergence. A somewhat similar trend was observed for glutathione with significant increase in embryo axes shortly before radicle protrusion followed by decline in the next hours. In cotyledons the ascorbate pool rose gradually during germination but the amount of glutathione showed fluctuations during a whole germination period. The activity of ascorbate peroxidase (APX) rose progressively in embryo axes, while activities of dehydroascorbate reductase (DHAR) and glutathione reductase (GR) showed transient increase during germination. New isoforms of APX and GR were synthesized, suggesting that they play a relevant role during germination. All analyzed enzymes were already present in dry seeds which allowed them to be active immediately after imbibition.

Keywords: antioxidative enzymes; Lupinus luteus L; radicle emergence
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; cotyledon, cotyledonary node; dehydroascorbate reductase; germination; glutathione reductase; in vitro culture, somatic embryogenesis; lupine; Lupinus luteus

Received: February 1, 2007; Accepted: July 1, 2007; Published: December 1, 2008Show citation

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Garnczarska, M., & Wojtyla, Ł. (2008). Ascorbate and glutathione metabolism in embryo axes and cotyledons of germinating lupine seeds. Biologia plantarum, 52(4), 681-686. doi: 10.1007/s10535-008-0131-3.
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