Biologia plantarum 52:687-694, 2008 | DOI: 10.1007/s10535-008-0133-1

Involvement of exopeptidases in dehydration tolerance of spring wheat seedlings

A. Miazek1, B. Zagdańska1,2,*
1 Biochemistry Department, Warsaw University of Life Sciences, Warszawa, Poland
2 Plant Physiology and Biochemistry Department, Plant Breeding and Acclimatization Institute, Radzików, Warszawa, Poland

The observed inability of 6-d-old seedlings of spring wheat (Triticum aestivum L.) to tolerate the same water deficit as compared to the 4-d-old seedlings seems to be associated with the higher carboxypeptidase and lower aminopeptidase activities. Free amino acid pools differentiated also the 4-d-old seedlings from the older ones. Dehydration decreased the amino acid content in 4-d-old seedlings, increased it in 6-d-old seedlings and changed composition of amino acid pool. In tolerant phase of wheat seedling growth carboxypeptidase activity increased in response to water deficit and aminopeptidase activity increased in dehydrated seedlings, independently of their age.

Keywords: aminopeptidases; carboxypeptidases; Triticum aestivum
Subjects: amino acids; ethylenediaminetetraacetic acid; in vitro culture, regeneration; Triticum aestivum; water content, relative (RWC); water stress; wheat

Received: November 15, 2006; Accepted: June 2, 2007; Published: December 1, 2008Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Miazek, A., & Zagdańska, B. (2008). Involvement of exopeptidases in dehydration tolerance of spring wheat seedlings. Biologia plantarum, 52(4), 687-694. doi: 10.1007/s10535-008-0133-1.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bartling, D., Nosek, J.: Molecular and immunological characterization of leucine aminopeptidase in Arabidopsis thaliana: a new antibody suggests a semi-constitutive regulation of a phylogenetically old enzyme.-Plant Sci 9: 199-209, 1994. Go to original source...
    2. Beers, E.P., Jones, A.M., Dickerman, A.W.: The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis.-Phytochemistry 65: 43-58, 2004. Go to original source...
    3. Blum, A., Sinmena, B., Ziv, O.: An evaluation of seed and seedling drought tolerance screening test in wheat.-Euphytica 29: 727-36, 1980. Go to original source...
    4. Bogdan, J., Zagdańska, B.: Drought resistance of spring wheat during germination and seedling growth.-Bull. IHAR 233: 73-80, 2004.
    5. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    6. Bray, E.A.: Plant responses to water deficit.-Trends Plant Sci. 2: 48-54, 1997. Go to original source...
    7. Callis, J.: Regulation of protein degradation.-Plant Cell 7: 845-57, 1995. Go to original source...
    8. Carrasco, P., Carbonell, J.: Changes in the level of peptidase activities in pea ovaries during senescence and fruit set induced by gibberellic acid.-Plant Physiol. 92: 1070-1074, 1990. Go to original source...
    9. Cercós, M., Urbez, C., Carbonell, J.: A serine carboxypeptidase gene (PsCP), expressed in early stages of reproductive and vegetative development in Pisum sativum, is induced by gibberellins.-Plant mol. Biol. 51: 165-174, 2003. Go to original source...
    10. Chao, W.S., Gu, Y.-Q., Pautot, V., Bray, E.A., Walling, L.L.: Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals-systemin, methyl jasmonate, and abscisic acid.-Plant Physiol. 120: 979-992, 1999. Go to original source...
    11. Cherian, S., Reddy, M.P., Ferreira, R.B.: Transgenic plants with improved dehydration-stress tolerance: progress and future prospects.-Biol. Plant. 50: 481-495, 2006. Go to original source...
    12. Cruz de Carvalho, M.H., d'Arcy-Lameta, A., Roy-Macauley, H., Gareil, M., El Maarouf, H., Pham Thi, A.T., Zuily-Fodil, Y.: Aspartic protease in leaves of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata L. Walp): enzymatic activity, gene expression and relation to drought susceptibility.-FEBS Lett. 492: 242-246, 2001. Go to original source...
    13. Dominguez, F., Cejudo, F.J. Germination-related genes encoding proteolytic enzymes are expressed in the nucellus of developing wheat grains.-Plant J. 15: 569-574, 1998. Go to original source...
    14. Dominguez, F., Gonzalez, M.C., Cejudo, F.J.: A germination-related gene encoding a serine carboxypeptidase is expressed during the differentiation of the vascular tissue in wheat grains and seedlings.-Planta 215: 727-734, 2002. Go to original source...
    15. Dubey, R.S., Rani, M.: Influence of NaCl salinity on the behavior of protease, aminopeptidase and carboxypeptidase in rice seedlings in relation to salt tolerance.-Aust. J. Plant Physiol. 30: 133-145, 1990.
    16. El-Amrani, A., Suire, C., Camara, B., Gaudillère, J.-P., Couée, I.: Purification and characterization of a novel aminopeptidase, plastidial alanine-aminopeptidase, from the cotyledons of etiolated sugar beet seedlings.-Plant Physiol. 109: 87-94, 1995. Go to original source...
    17. Farrant, J.M., Bailly, C., Leymarie, J., Hamman, B., Ccme, D., Corbineau, F.: Wheat seedlings as a model to understand desiccation tolerance and sensitivity.-Physiol. Plant. 120: 563-574, 2004. Go to original source...
    18. Feller, U.K., Soong, T.-T., Hageman, R.H.: Patterns of proteolytic enzyme activities in different tissues of germinating corn (Zea mays L.).-Planta 140: 155-162, 1978. Go to original source...
    19. Foyer, C., Valadier, M.-H., Migge, A., Becker, T.W.: Drought-induced effects on nitrate reductase activity and mRNA and on the coordination of nitrogen and carbon metabolism in maize leaves.-Plant Physiol. 117: 283-292, 1998. Go to original source...
    20. Grudkowska, M., Zagdańska, B.: Multifunctional role of plant cysteine proteinases.-Acta Biochim. Polon. 51: 609-624, 2004. Go to original source...
    21. Guerida, M., Shroyer, J.P., Kirkham, M.B., Paulsen, G.M.: Wheat coleoptile and root growth and seedling survival after dehydration and rehydration.-Agron. J. 89: 822-826, 1997.
    22. Guerrero, F.D., Jones, J.T., Mullet, J.E.: Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted and expression of three inducible genes.-Plant mol. Biol. 15: 11-26, 1990. Go to original source...
    23. Hieng, B., Ugrinović, K., Šuštar-Vozlič, J., Kidrič, M.: Different classes of proteases are involved in the response to drought of Phaseolus vulgaris L. cultivars differing in sensitivity.-J Plant Physiol. 161: 519-530, 2004. Go to original source...
    24. Hildmann, T., Ebneth, M., Pena-Cortes, H., Sanchez-Serrano, J.J., Willmitzer, L., Prat, S.: General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding.-Plant Cell 4: 1157-1170, 1992. Go to original source...
    25. Ingram, J., Bartels, D.: The molecular basis of dehydration tolerance in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 377-403, 1996. Go to original source...
    26. Kolehmainen, L., Mikola, J.: Partial purification and enzymatic properties of an aminopeptidase from barley.-Arch. Biochem. Biophys. 145: 633-642, 1971. Go to original source...
    27. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    28. Mehta, R.A., Warmbardt, R.D., Mattoo, A.K.: Tomato fruit carboxypeptidase. Properties, induction upon wounding, and immunocytochemical localization.-Plant Physiol. 110: 883-892, 1996. Go to original source...
    29. Miazek, A., Bogdan, J., Zagdańska, B.: Effect of water deficit during germination of wheat seeds.-Biol. Plant. 44: 397-403, 2001. Go to original source...
    30. Li, J., Lease, K.A., Tax, F.E., Walker, J.C. BRS1, a serine carboxypeptidase, regulates BRII signaling in Arabidopsis.-Proc. nat. Acad. Sci USA 98: 5916-5921, 2001. Go to original source...
    31. Mikola, J., Kolehmainen, L: Localization of activity of various peptidases in germinating barley.-Planta 104: 167-177, 1972. Go to original source...
    32. Mikola, L., Mikola, J.: Occurrence and properties of different types of peptidases in higher plants.-In: Dalling, M.J. (ed.): Plant Proteolytic Enzymes. Vol. 1. Pp. 97-117. CRC Press, Boca Raton 1986.
    33. Navari-Izzo, F., Quartacci, M.F., Izzo, R.: Water-stress induced changes in protein and free amino acids in field-grown maize and sunflower.-Plant Physiol. Biochem. 28: 531-537, 1990.
    34. Ogiwara, N., Amano, T., Satoh, M., Shioi, Y.: Leucine aminopeptidase from etiolated barley seedlings: characterization and partial purification of isoforms.-Plant Sci 168: 575-581, 2005. Go to original source...
    35. Palma, J.M., Sandalio, L.M., Corpas, F.J., Romero-Puertas, M.C., McCarthy, I., Del Rio, L.A.: Plant proteases, protein degradation, and oxidative stress: role of peroxisomes.-Plant Physiol. Biochem. 40: 521-30, 2002. Go to original source...
    36. Parrott, D., Yang, L., Shama, L., Fischer, A.M.: Senescence is accelerated, and several proteases are induced by carbon "feast" conditions in barley (Hordeum vulgare L.) leaves.-Planta 222: 989-1000, 2005. Go to original source...
    37. Ramirez-Zavala, B., Mercado-Flores, Y., Hernandez-Rodriguez, C., Villa-Tanaca, L.: Purification and characterization of a serine carboxypeptidase from Kluyveromycetes marxianus.-Int. J. Food Microbiol. 91: 245-252, 2004. Go to original source...
    38. Roy-Macauley, H., Zuily-Fodil, Y., Kidrič, M., Pham Thi, A.T., Veira da Silva, J.: Effect of drought stress on proteolytic activities in Phaseolus and Vigna leaves from sensitive and resistant plants.-Physiol. Plant. 85: 90-96, 1992. Go to original source...
    39. Schaffer, M.A., Fischer, R.L.: Analysis of mRNA that accumulates in response to low temperature identifies a thiol protease gene in tomato.-Plant Physiol. 87: 431-436, 1988. Go to original source...
    40. Schaffer, M.A., Fischer, R.L.: Transcriptional activation by heat and cold of a thiol protease gene in tomato.-Plant Physiol. 93: 1486-1492, 1990. Go to original source...
    41. Schaller, A., Ryan, C.A.: Systemin: a polypeptide defense signal in plants.-BioEssays 18: 27-33, 1995. Go to original source...
    42. Schaller, A.: A cut above the rest: the regulatory function of plant proteases.-Planta 220: 183-197, 2004. Go to original source...
    43. Simon-Sarkadi, I., Kocsy, G., Varhegyi, A., Galiba, G., De Ronde, J.A.: Stress-induced changes in the free amino acid composition in transgenic soybean plants having increased proline content.-Biol. Plant. 50: 793-796, 2006. Go to original source...
    44. Thayer, S.S., Choe, H.T., Rausser, S., Huffaker, R.C.: Characterization and subcellular localization of aminopeptidases in senescing barley leaves.-Plant Physiol. 87: 894-897, 1988. Go to original source...
    45. Tonecki, J., Gorin, N., Verghagen, W.: Quantitative high-performance liquid chromatography and enzymatic estimation of four free amino acids in powders of anthers from bulbs of tulip.-J. Liquid Chrom. 12: 2321-2331, 1989.
    46. Varshavsky, A.: The N-end rule: functions, mysteries, uses.-Proc. nat. Acad. Sci USA 93: 12142-12149, 1996. Go to original source...
    47. Vierstra, R.D.: Proteolysis in plants: mechanisms and functions.-Plant mol. Biol. 32: 275-302, 2004.
    48. Vincent, J.L., Brewin, N.J.: Immunolocalization of a cysteine protease in vacuoles, vesicles and symbiosomes of pea nodule cells.-Plant Physiol. 123: 521-530, 2000. Go to original source...
    49. Vodkin, L., Scandalios, J.G.: Comparative properties of genetically defined peptidases in maize.-Biochemistry 19: 4660-4667, 1980. Go to original source...
    50. Walling, L.L.: Recycling or regulation? The role of amino-terminal modifying enzyme.-Curr. Opin. Plant Biol. 9: 227-233, 2006.
    51. Wilson, K.A.: Role of proteolytic enzymes in the mobilization of protein reserves in the germinating dicot seed.-In: Dalling, M.J. (ed.): Plant Proteolytic Enzymes. CRC Press, Boca Raton 1986.
    52. Wiśniewski, K., Zagdańska, B.: Genotype-dependent proteolytic response of spring wheat to water deficiency.-J. exp. Bot. 52: 1455-66, 2001. Go to original source...
    53. Zagdańska, B., Neumann, M.: Amino acids changes in drought-affected spring wheat.-Bull. Pol. Acad. Sci. 32: 229-235, 1984.

    Return to the content