Biologia plantarum 53:63-68, 2009 | DOI: 10.1007/s10535-009-0009-z

Calcium is involved in the abscisic acid-induced ascorbate peroxidase, superoxide dismutase and chilling resistance in Stylosanthes guianensis

B. Zhou1,2, Z. Guo1,*
1 Biotechnology Laboratory for Turfgrass and Forages, College of Life Science, South China Agricultural University, Guangzhou, P.R. China
2 College of Horticulture, South China Agricultural University, Guangzhou, P.R. China

The objective of this work was to test whether Ca2+, a second messenger in stress response, is involved in ABA-induced antioxidant enzyme activities in Stylosanthes guianensis. Plants were sprayed with abscisic acid (ABA), calcium channel blocker, LaCl3, calcium chelator, ethylene glycol-bis(β-amino ethyl ether)-N,N,N', N'-tetraacetid acid (EGTA), and ABA in combination with LaCl3 or EGTA. Their effects on superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities and chilling resistance were compared. The results showed that ABA decreased electrolyte leakage and lipid peroxidation but increased maximum photochemical efficiency measured as variable to maximum fluorescence ratio (Fv/Fm) under chilling stress. Treatment with LaCl3 or EGTA alone and in combination with ABA increased electrolyte leakage and lipid peroxidation, decreased Fv/Fm, suggesting that the block in Ca2+ signalling decreased chilling resistance of S. guianensis and the ABA-enhanced chilling resistance. ABA-induced SOD and APX activities were suppressed by LaCl3 or EGTA. The results suggested that Ca2+ is involved in the ABA-enhanced chilling resistance and the ABA-induced SOD and APX activities in S. guianensis.

Keywords: chlorophyll fluorescence; electrolyte leakage; EGTA; LaCl3; lipid peroxidation
Subjects: abscisic acid (ABA); ascorbate peroxidase; calcium; chlorophyll fluorescence; electrolyte leakage; lipid peroxidation; malondialdehyde; photochemical efficiency; photosystems; Stylosanthes gaianensis; superoxide dismutase (SOD); temperature, low

Received: January 15, 2007; Accepted: August 21, 2007; Published: March 1, 2009Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Zhou, B., & Guo, Z. (2009). Calcium is involved in the abscisic acid-induced ascorbate peroxidase, superoxide dismutase and chilling resistance in Stylosanthes guianensis. Biologia plantarum, 53(1), 63-68. doi: 10.1007/s10535-009-0009-z.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Agarwal, S., Sairam, R.K., Srivastava, G.C., Tyagi, A., Meena, R.C.: Role of ABA, salicylic acid, calcium and hydrogen peroxide on antioxidant enzymes induction in wheat seedlings.-Plant Sci. 169: 559-570, 2005. Go to original source...
    2. Anderson, M.D., Prasad, T.K., Martin, B.A., Stewart, C.R.: Differential gene expression in chilling acclimated maize seedlings and evidence for the involvement of abscisic acid in chilling tolerance.-Plant Physiol. 105: 331-339, 1994. Go to original source...
    3. Bertamini, M., Zulini, L., Muthuchelian, K., Nedunchezhian, N.: Low night temperature effects on photosynthetic performance on two grapevine genotypes.-Biol. Plant. 51: 381-385, 2007. Go to original source...
    4. Bertin, P., Bouharmont, J., Kinet, J.M.: Somaclonal variation and improvement of chilling tolerance in rice: Changes in chilling-induced chlorophyll fluorescence.-Crop Sci. 37: 1727-1735, 1997. Go to original source...
    5. Bowler, C., Van Montagu, M., Inze, D.: Superoxide dismutase and stress tolerance.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83-116, 1992. Go to original source...
    6. Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    7. Bravo, L.A., Zúńiga, G.E., Alberdi, M., Corcuera, L.J.: The role of ABA in freezing tolerance and cold acclimation.-Physiol. Plant. 103: 12-23, 1998. Go to original source...
    8. Bush, D.S.: Calcium regulation in plant cell and its role in signalling.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 122-137, 1995. Go to original source...
    9. Cousson, A.: Two calcium mobilizing pathways implicated within abscisic acid induced stomatal closing in Arabidopsis thaliana.-Biol. Plant. 52: 285-291, 2007. Go to original source...
    10. Fadzillah, N.M., Gill, V., Finch, B.P., Burdon, R.H.: Chilling, oxidative stress and antioxidant responses in shoot cultures of rice.-Planta 199: 552-556, 1996. Go to original source...
    11. Gong, M., Luit, A.H., Knight, M.R., Trewavas, A.J.: Heat-shock-induced changes in intracellular Ca2+ in tobacco seedling in relation to thermotolerance.-Plant Physiol. 116: 429-437, 1998a. Go to original source...
    12. Gong, M., Li, Y.-J., Chen, S.-Z.: Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems.-J. Plant Physiol. 153: 488-496, 1998b. Go to original source...
    13. Guan, L., Scandalios, J.G.: Cis-elements and transfactors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is likely intermediary signalling molecule for the response.-Plant J. 22: 87-95, 2000. Go to original source...
    14. Guo, Z., Huang, M., Lu, S., Zhao, Y., Zhong, Q.: Differential response to paraquat induced oxidative stress in two rice cultivars on antioxidants and chlorophyll a fluorescence.-Acta Physiol. Plant. 29: 39-46, 2007.
    15. Guo, Z., Ou, W., Lu, S., Zhong, Q.: Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity.-Plant Physiol. Biochem. 44: 828-836, 2006. Go to original source...
    16. Huang, M., Guo, Z.: Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity.-Biol Plant. 49: 81-84, 2005. Go to original source...
    17. Jiang, Y.W., Huang, B.G.: Effects of calcium on antioxidant activities and water relations associated with heat tolerance in two cool-season grasses.-J. exp. Bot. 35: 341-349, 2001. Go to original source...
    18. Jiang, M., Zhang, J.: Effects of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedling.-Plant Cell Physiol. 42: 1265-1273, 2001. Go to original source...
    19. Jiang, M., Zhang, J.: Role of abscisic acid in water stress-induced antioxidant defense in leaves of maize seedlings.-Free Radical Res. 36: 1001-1015, 2002a. Go to original source...
    20. Jiang, M., Zhang, J.: Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves.-J. exp. Bot. 379: 2401-2410, 2002b. Go to original source...
    21. Jiang, M., Zhang, J.: Cross-talk between calcium and reactive oxygen species originated from NADPH oxidase in abscisic acid-induced antioxidant defence in leaves of maize seedlings.-Plant Cell Environ. 26: 929-939, 2003. Go to original source...
    22. Hu, X., Jiang, M., Zhang, A., Lu, J.: Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves.-Planta 223: 57-68, 2005. Go to original source...
    23. Krol, E., Dziubinska, H., Stolarz, M., Trebacz, K.: Effect of ion channel inhibitors on cold-and electrically-induced action potentials in Dionaea muscipula.-Biol. Plant. 50: 411-416, 2007.
    24. Larkindale, J., Huang, B.: Thermotolerance and antioxidant systems in Agrostis stolonifera: involvement of salicylic acid, abscisic acid, calcium, hydrogen peroxide, and ethylene.-J. Plant Physiol. 161: 405-413, 2004. Go to original source...
    25. Lin, D.I., Lur, H.S., Chu, C.: Effects of abscisic acid on ozone tolerance of rice (Oryza sativa L.) seedlings.-Plant Growth Regul. 35: 295-300, 2001. Go to original source...
    26. Lu, S., Guo, Z., Lu, J., Zhong, J., Han, L.: Effect of abscisic acid on chilling injury of centipedegrass.-Int. Turfgrass Soc. Res. J. 10: 862-8661, 2005.
    27. Lu, S., Guo, Z., Peng, X.: Effects of ABA and S-3307 on drought resistance and antioxidative enzyme activity of turfgrass.-J. hort. Sci. Biotechnol. 78: 663-666, 2003. Go to original source...
    28. Meijer, E.G.M., Broughton, W.J.: Regeneration of whole plants from hypocotyl-, root-, and leaf-derived tissue cultures of the pasture legume Stylosanthes guianensis.-Physiol. Plant. 52: 280-248, 1981. Go to original source...
    29. Miles, J.W., Grof, B.: Recent advances in studies of anthracnose of Stylosanthes. III. Stylosanthes breeding approaches in South America.-Trop. Grasslands 31: 430-434, 1997.
    30. Miles, J.W., Lascano, C.E.: Status of Stylosanthes development in other countries. I. Stylosanthes development and utilisation in South America.-Trop. Grasslands 31: 454-459, 1997.
    31. Monroy, A.F., Dhindsa, R.S.: Low-temperature signal transduction: induction of cold acclimation-specific genes of alfalfa by calcium at 25 °C.-Plant Cell 7: 321-331, 1995.
    32. Murata, Y., Pei, Z.M., Mori, I.C., Schroeder, J.I.: Abscisic acid activation of plasma membrane Ca2+ channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abil-1_and abi2-1 protein phosphatase 2C mutants.-Plant Cell 13: 2513-2523, 2001. Go to original source...
    33. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
    34. Pei, Z.M., Murata, Y., Benning, G., Thomine, S., Klüsener, B., Allen, G.J., Grill, E., Schroeder, J.I.: Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells.-Nature 406: 731-734, 2000. Go to original source...
    35. Prasad, T.K., Anderson M.D., Martin B.A., Stewart C.R.: Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide.-Plant Cell 6: 65-74, 1994. Go to original source...
    36. Tester, M.: Plant ion channels-whole cell and single channel studies.-New Phytol. 114: 305-340, 1990. Go to original source...
    37. Yoshida, K., Igaashi, E., Waatsuki E., Miyamoto, K., Hirata, K.: Mitigation of osmotic and salt stresses by abscisic acid through reduction of stress-derived oxidative damage in Chlamydomonas reinhardtii.-Plant Sci. 167: 1335-1341, 2004. Go to original source...
    38. Zhou, B., Guo, Z., Liu, Z.: Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress.-Crop Sci. 45: 599-605, 2005a. Go to original source...
    39. Zhou, B., Guo, Z., Xing, J., Huang, B.: Nitric oxide is involved in abscisic acid-induced antioxidant activities in Stylosanthes guianensis.-J. exp. Bot. 56: 3223-3228, 2005b. Go to original source...
    40. Zhou, B., Guo, Z. Lin, L.: Effects of abscisic acid application on photosynthesis and photochemistry of Stylosanthes guianensis under chilling stress.-Plant Growth Regul. 48: 195-199, 2006.
    41. Zhou, B.-Y., Guo, Z.-F.: Effect of ABA and its biosynthesis inhibitor on chilling resistance and antioxidant enzymes activity.-Acta prataculturae sin. 14: 94-99, 2005.
    42. Zhou, B.-Y., Li, Y.-B., Chen, J.-Z., Ji, Z.-L., Hu, Z.-Q.: Effects of low temperature stress on flower formation and endogenous hormone of litchi.-Acta hort. sin. 29: 577-578, 2002.
    43. Zhu, D., Scandalios, J.G.: Differential accumulation of manganese-superoxide dismutase transcripts in maize in response to abscisic acid and high osmoticum.-Plant Physiol. 106: 173-178, 1994. Go to original source...
    44. Zong, H., Xu, Z.-L., Liu, E.-E., Guo, Z.-F.: Effects of chlorpromazine and lanthanum chloride on proline accumulation in rice seedlings under cold stress.-J. trop. subtrop. Bot. 11: 241-244, 2003.

    Return to the content