Biologia plantarum 53:365-368, 2009 | DOI: 10.1007/s10535-009-0068-1

Low doses of ultraviolet-B or ultraviolet-C radiation affect phytohormones in young pea plants

Z. Katerova1,*, S. Ivanov2, E. Prinsen3, H. Van Onckelen3, V. Alexieva1, A. Azmi3
1 Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Centre of Food Biology, Sofia, Bulgaria
3 Department of Biology, University of Antwerp, Antwerp, Belgium

Pea (Pisum sativum L., cv. Scinado) seedlings were exposed to low doses of ultraviolet-B (UV-B; 4.4 and 13.3 kJ m-2 d-1) or UV-C (0.1 and 0.3 kJ m-2 d-1) radiation for 14 d. Aminocyclopropane carboxylic acid (ACC), indoleacetic acid (IAA) and abscisic acid (ABA) contents were quantified by gas chromatography coupled to mass spectrometry (GC-MS). The accumulation of ACC upon irradiation was dose-dependent. ABA content was reduced and IAA content increased upon UV-C treatment whereas the UV-B doses used did not cause significant changes in ABA and IAA contents.

Keywords: abscisic acid; aminocyclopropane carboxylic acid; indoleacetic acid; Pisum sativum; stress
Subjects: abscisic acid (ABA); aminocyclopropane carboxylic acid (ACC); auxins; pea; Pisum sativum; UV radiation

Received: March 3, 2008; Accepted: July 5, 2008; Published: June 1, 2009Show citation

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Katerova, Z., Ivanov, S., Prinsen, E., Onckelen, H., Alexieva, V., & Azmi, A. (2009). Low doses of ultraviolet-B or ultraviolet-C radiation affect phytohormones in young pea plants. Biologia plantarum, 53(2), 365-368. doi: 10.1007/s10535-009-0068-1.
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    References

    1. Abeles, F.B.: Auxin stimulation of ethylene evolution.-Plant Physiol. 41: 585-588, 1966. Go to original source...
    2. Agarwal, S.: Increased antioxidant activity in Cassia seedlings under UV-B radiation.-Biol. Plant. 51: 157-160, 2007. Go to original source...
    3. Albinsky, D., Masson, J.E., Bogocki, A., Afsar, K., Vass, I., Nagy, F., Paszkowski, J.: Plant responses to genotoxic stress are linked to an ABA/salinity signalling pathway.-Plant J. 17: 73-82, 1999. Go to original source...
    4. Caldwell, M.M.: Solar ultraviolet radiation and the growth and development of higher plants.-In: Giese, A.C. (ed.): Photophysiology. Pp. 131-177. Academic Press, New York 1971. Go to original source...
    5. Córdoba, C., Munoz, J.A., Cachorro, V., De Carcer, I.A., Cusso, F., Jaque, F.J.: The detection of solar ultraviolet-C radiation using KCl:Eu2+ thermoluminescence dosemeters.-J. Phys. D: appl. Phys. 30: 3024-3027, 1997.
    6. Häder, D.-P., Kumar, H.D., Smith, R.C., Worrest, R.C.: Effects of solar UV radiation on aquatic ecosystems and interactions with climate change.-Photochem. Photobiol. Sci. 6: 267-285, 2007. Go to original source...
    7. Huang, S., Dai, Q., Peng, S., Chavez, A.Q., Miranda, M.L.L., Visperas, R.M., Vergara, B.S.: Influence of supplemental ultraviolet-B on indoleacetic acid and calmodulin in the leaves of rice (Oryza sativa L.).-Plant Growth Regul. 21: 59-64, 1997. Go to original source...
    8. Hyodo, H., Tanaka, K., Yoshisaka, J.: Induction of 1-aminocyclopropane-1-carboxylic acid synthase in wounded mesocarp tissue of winter squash and the effects of ethylene.-Plant Cell Physiol. 26: 161-167, 1985.
    9. Jansen, M.A., Den Noort, R.E., Tan, M.Y., Prinsen, E., Lagrimini, L.M., Thorneley, R.N.: Phenol-oxidizing peroxidases contribute to the protection of plants from ultraviolet radiation stress.-Plant Physiol. 126: 1012-1023, 2001. Go to original source...
    10. Ljung, K., Östin, A., Lioussanne, L., Sandberg, G.: Developmental regulation of indole-3-acetic acid turnover in Scots pine seedlings.-Plant Physiol. 125: 464-475, 2001. Go to original source...
    11. Milborrow, B.V.: The pathway of biosynthesis of abscisic acid in vascular plants: a review of the present state of knowledge of ABA biosynthesis.-J. exp. Bot. 52: 1145-1164, 2001 Go to original source...
    12. Nakajima, N., Itoh, T., Takikawa, S., Asai, N., Tamaoki, M., Aono, M., Kubo, A., Azumi, Y., Kamada, H., Saji, H.: Improvement in ozone tolerance of tobacco plants with an antisense DNA for 1-aminocyclopropane-1-carboxylate synthase.-Plant Cell Environ. 25: 727-735, 2002. Go to original source...
    13. Nara, A., Takeuchi, Y.: Ethylene evolution from tobacco leaves irradiated with UV-B.-J. Plant Res. 115: 247-253, 2002. Go to original source...
    14. Paul, N.: Plant responses to UV-B: time to look beyond stratospheric ozone depletion?-New Phytol. 150: 5-8, 2001. Go to original source...
    15. Persson, J., Näsholm, T.: A GC-MS method for determination of amino acid uptake by plants.-Physiol. Plant. 113: 352-358, 2001. Go to original source...
    16. Prinsen, E., Rüdelsheim, P., Van Onckelen, H.: Extraction, purification and analysis of endogenous indole-3-acetic acid and abscissic acid.-In: Negrutiu, I., Gharti-Chhetri, G.B. (ed.): A Laboratory Guide for Cellular and Molecular Plant Biology. Pp. 198-209. Birkhäuser-Verlag, Basel 1991.
    17. Rakitina, T.Y., Vlasov, P.V., Rakitin, V.: Hormonal aspects of different resistance of Arabidopsis thaliana mutants to ultraviolet radiation.-Russ. J. Plant. Physiol. 48: 353-358, 2001.
    18. Rodrigo, M.-J., Marcos, J.F., Alférez, F., Mallent, M.D., Zacarías, L.: Characterization of pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content.-J. exp. Bot. 54: 727-738, 2003. Go to original source...
    19. Skórska, E., Szwarc, W.: Influence of UV-B radiation on young triticale plants with different wax cover.-Biol. Plant. 51: 189-192, 2007. Go to original source...
    20. Shama, G., Alderson, P.: UV hormesis in fruits: a concept ripe for commercialisation.-Trends Food Sci. Technol. 16: 128-136, 2005. Go to original source...
    21. Smets, R., Claes, V., Van Onckelen, H., Prinsen, E.: Extraction and quantitative analysis of 1-aminocyclopropane-1-carboxylic acid in plant tissue by gas chromatography coupled to mass spectrometry.-J. Chromatogr. A 993: 79-87, 2003. Go to original source...
    22. Stapleton, A.E.: Ultraviolet radiation and plants: burning questions.-Plant Cell 4: 1353-1358, 1992. Go to original source...
    23. Todoroki, Y., Tanaka, T., Kisamori, M., Hirai, N.: 3'-azidoabscisic acid as a photoaffinity reagent for abscisic acid binding proteins.-Bioorg. med. chem. Lett. 11: 2381-2384, 2001. Go to original source...
    24. Wang, S.Y., Wang, C.Y., Welburn, A.R.: Role of ethylene under stress conditions.-In: Alscher, R., Cumming, J. (ed.): Stress Responses in Plants Adaptation and Acclimation Mechanisms. Pp. 147-173. Wiley-Liss, New York 1990.
    25. Yang, H., Zhao, Z., Qiang, W., An, L., Xu, S., Wang, X.: Effects of enhanced UV-B radiation on the hormonal content of vegetative and reproductive tissues of two tomato cultivars and their relationships with reproductive characteristics.-Plant Growth Regul. 43: 251-258, 2004. Go to original source...
    26. Yu, Y.B., Adams, D.O., Yang, S.F.: 1-aminocyclopropane-carboxylate synthase, a key enzyme in ethylene biosinthesis of tomatoes.-Arch. Biochim. Biophys. 198: 280-286, 1979. Go to original source...
    27. Yu, Y.B., Yang, S.F.: Biosynthesis of wound ethylene mung beans, orange, tomato.-Plant Physiol. 66: 281-285, 1980. Go to original source...

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