Biologia plantarum 54:259-264, 2010 | DOI: 10.1007/s10535-010-0045-8

GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes

I. Molnár1, M. Molnár-Láng1,*
1 Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary

The Triticum aestivum - Aegilops biuncialis (2n=4x=28; UbUbMbMb) disomic addition lines 2Mb, 3Mb, 7Mb and 3Ub were crossed with the wheat cv. Chinese Spring ph1b mutant genotype in order to induce homoeologous pairing, with the final goal of introgressing Ae. biuncialis chromatin into cultivated wheat. Wheat-Aegilops homoeologous chromosome pairing was studied in metaphase I of meiosis in the F1 hybrid lines. Using U and M genomic probes, genomic in situ hybridization (GISH) demonstrated the occurrence of wheat-Aegilops homoeologous pairing in the case of chromosomes 2Mb, 3Mb and 3Ub, but not in the case of 7Mb. The wheat-Aegilops pairing frequency decreased in the following order: 2Mb > 3Mb > 3Ub > 7Mb, which may reflect differences in the wheat-Aegilops homoeologous relationships between the examined Aegilops chromosomes. The selection of wheat-Aegilops homoeologous recombinations could be successful in later generations.

Keywords: disomic addition lines; ph1b mutant; Triticum aestivum
Subjects: Aegilops biuncialis; chromosome characteristics; chromosome pairing; disomic addition lines; genomic in situ hybridization (GISH); mutagenesis, mutants; Triticum aestivum; wheat

Received: June 28, 2008; Accepted: January 15, 2009; Published: June 1, 2010Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Molnár, I., & Molnár-Láng, M. (2010). GISH reveals different levels of meiotic pairing with wheat for individual Aegilops biuncialis chromosomes. Biologia plantarum, 54(2), 259-264. doi: 10.1007/s10535-010-0045-8.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bálint, A. F., Röder, M. S., Hell, R., Galiba, G., Börner, A.: Mapping of QTLs affecting copper tolerance and the Cu, Fe, Mn and Zn contents in the shoots of wheat seedlings. - Biol. Plant. 51: 129-134, 2007. Go to original source...
    2. Benavente, E., Fernandez-Calvin, B., Orellana, J.: Relationship between the levels of wheat-rye metaphase I chromosomal pairing and recombination revealed by GISH. - Chromosoma 105: 92-96, 1996. Go to original source...
    3. Benavente, E., Alix, K., Dusautoir, J.C., Orellana, J., David, J.L.: Early evolution of the chromosomal structure of Triticum turgidum - Aegilops ovata amphiploids carrying and lacking the Ph1 gene. - Theor. appl. Genet. 103: 1123-1128, 2001. Go to original source...
    4. Cattivelli, L., Baldi, P., Crosatti, C., Di Fonzo, N., Faccioli, P., Grossi, M., Mastrangelo, A.M., Pecchioni, N., Stanca, A.M.: Chromosome regions and stress-related sequences involved in resistance to abiotic stress in Triticeae. - Plant mol. Biol. 48: 649-665, 2002. Go to original source...
    5. Cuadrado, A., Vitellozzi, F., Jouve, N., Ceoloni, C.: Fluorescence in situ hybridization with multiple repeated DNA probes applied to the analysis of wheat-rye chromosome pairing. - Theor. appl. Genet. 94: 347-355, 1997. Go to original source...
    6. Damania, A.B., Pecetti, L.: Variability in a collection of Aegilops species and evaluation for yellow rust resistance at two locations in Northern Syria. - J. Genet. Breed. 44: 97-102, 1990.
    7. Dimov, A., Zaharieva, M., Mihova, S.: Rust and powdery mildew resistance in Aegilops accessions from Bulgaria. - In: Damania, A.B. (ed.): Biodiversity and Wheat Improvement. Pp. 165-169. John Wiley & Sons, Chichester 1993.
    8. Friebe, B., Jiang, J., Raupp, W.J., McIntosh, R.A., Gill, B.S.: Characterization of wheat alien translocations conferring resistance to diseases and pests: current status. - Euphytica 71: 59-87, 1996a. Go to original source...
    9. Friebe, B., Jiang, J., Tuleen, N., Gill, B.S.: Standard karyotype of Triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in common wheat. - Theor. appl. Genet. 90: 150-156, 1995. Go to original source...
    10. Friebe, B., Kynast, R.G., Gill, B.S.: Gametocidal factor-induced structural rearrangements in rye chromosomes added to common wheat. - Chromosome Res. 8: 501-511, 2000. Go to original source...
    11. Friebe, B., Tuleen, N.A., Badaeva, E.D., Gill, B.S.: Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat. - Genome 39: 272-276, 1996b. Go to original source...
    12. Friebe, B., Tuleen, N.A., Gill, B.S.: Development and identification of a complete set of Triticum aestivum-Aegilops geniculata chromosome addition lines. - Genome 42: 374-380, 1999. Go to original source...
    13. Gold, J., Harder, D., Townley-Smith, F., Aung, T., Procunier, J.: Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. - Electronic J. Biotechnol. 2: 1-6, 1999.
    14. Irick, H.: A new function for heterochromatin. - Chromosoma 103: 1-3, 1994. Go to original source...
    15. Kuraparthy, V., Chhuneja, P., Dhaliwal, H.S., Kaur, S., Bowden, R.L., Gill, B.S.: Characterization and mapping of cryptic introgression from Ae. geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. - Theor. appl. Genet. 114: 1379-1389, 2007. Go to original source...
    16. Landjeva, S., Merakchijska-Nikolova, M., Ganeva, G.: Copper toxicity tolerance in Aegilops and Haynaldia seedlings. - Biol. Plant. 46: 479-480, 2003. Go to original source...
    17. Le, H.T., Armstrong, K.C., Miki, B.: Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. - Plant. mol. Biol. Rep. 7: 150-158, 1989. Go to original source...
    18. Logojan, A., Molnár-Láng, M.: Production of Triticum aestivum-Aegilops biuncialis chromosome additions. - Cereal Res. Commun. 28: 221-228, 2000. Go to original source...
    19. Lukaszewski, A.J.: Manipulation of the 1RS.1BL translocation in wheat by induced homoelogous recombination. - Crop Sci. 40: 216-225, 2000. Go to original source...
    20. Makkouk, K.M., Comeau, A., Ghulam, W.: Resistance to barley yellow dwarf luteovirus in Aegilops species. - Can. J. Plant Sci. 74: 631-634, 1994. Go to original source...
    21. Maestra, R., Naranjo, T.: Genome evolution in Triticeae. - In: Olmo, E., Redi, C.A. (ed.): Chromosomes Today. Vol. 13. Pp. 155-167. Birkhaüser Verlag, Basel 2000. Go to original source...
    22. McIntosh, R.A., Miller, T.E., Chapman, V.: Cytogenetical studies in wheat XII. Lr28 for resistance to Puccinia recondita and Sr34 for resistance to P. gramini tritici. - Z. Pflanzenzucht. 89: 295-306, 1982.
    23. Miller, T.E., Reader, S.M., Purdie, K.A., King, I.P.: Determination of the frequency of wheat-rye chromosome pairing in wheat × rye hybrids with and without chromosome 5B. - Theor. appl. Genet. 89: 255-258, 1994. Go to original source...
    24. Molnár, I., Gáspár, L., Sárvári, É., Dulai, S., Hoffmann, B., Molnár-Láng, M., Galiba, G.: Physiological and morphological responses to water stress in Aegilops biuncialis and Triticum aestivum genotypes with differing tolerance to drought. - Funct. Plant Biol. 31: 1149-1159, 2004. Go to original source...
    25. Molnár, I., Schneider, A., Molnar-Láng, M.: Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences. - Cereal Res. Commun. 33: 673-680, 2005. Go to original source...
    26. Molnár-Láng, M., Linc, G., Friebe, B.R., Sutka, J.: Detection of wheat-barley translocations by genomic in situ hybridization in derivatives of hybrids multiplied in vitro. - Euphytica 112: 117-123, 2000. Go to original source...
    27. Molnár-Láng, M., Novotny, C., Linc, G., Nagy, E.: Changes in the meiotic pairing behaviour of a winter wheat-winter barley hybrid maintained for a long term tissue culture, and tracing the barley chromatin in the progeny using GISH and SSR markers. - Plant Breed. 124: 247-252, 2005. Go to original source...
    28. Pedersen, C., Langridge, P.: Identification of the entire chromosome complement of bread wheat by two-colour FISH. - Genome 40: 589-593, 1997. Go to original source...
    29. Rekika, D., Monneveux, P., Havaux, M.: The in vivo tolerance of photosynthetic membranes to high and low temperatures in cultivated and wild wheats of the Triticum and Aegilops genera. - J. Plant Physiol. 150: 734-738, 1997. Go to original source...
    30. Riley, R., Chapman, V.: Genetic control of cytologically diploid behaviour of hexaploid wheat. - Nature 182: 713-715, 1958. Go to original source...
    31. Sánchez-Morán, E., Benavente, E., Orellana, J.: Analysis of karyotypic stability of homoeologous-pairing (ph) mutants in allopolyploid wheats. - Chromosoma 110: 371-377, 2001. Go to original source...
    32. Schneider, A., Linc, G., Molnár, I., Molnár-Láng, M.: Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of five derived wheat/Aegilops biuncialis disomic addition lines. - Genome 48: 1070-1082, 2005. Go to original source...
    33. Schneider, A., Molnár, I., Molnár-Láng, M.: Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. - Euphytica 163: 1-19, 2008. Go to original source...
    34. Schwarzacher, T., Leitch, A.R., Bennett, M.D., Heslop-Harrison, J.S.: In situ localization of parental genomes in a wild hybrid. - Ann. Bot. 64: 315-324, 1989. Go to original source...
    35. Sears, E.R.: An induced mutant with homoeologous pairing in common wheat. - Can. J. Genet. Cytol. 19: 585-593, 1977. Go to original source...
    36. Sharp, P.J., Kreis, M., Shewry, P.R., Gale, M.D.: Location of β-amylase sequences in wheat and its relatives. - Theor. appl. Genet. 75: 286-290, 1988. Go to original source...
    37. Sybenga, J.: What makes homologous chromosomes find each other in meiosis? A review and an hypothesis. - Chromosoma 108: 209-219, 1999. Go to original source...
    38. Vrána, J., Kubaláková, M., Šimková, H., Číhalíková, J., Lysák, M.A., Doležel, J.: Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.). - Genetics 156: 2033-2041, 2000.
    39. Waldron, B.L., Moreno-Sevilla, B., Andersonm, J.A., Stack, R.W., Frohberg, R.C.: RFLP mapping of QTL for Fusarium head blight resistance in wheat. - Crop Sci. 39: 805-811, 1999. Go to original source...
    40. Zaharieva, M., Gaulin, E., Havaux, M., Acevedo, E., Monneveux, P.: Drought and heat responses in the wild wheat relative Aegilops geniculata Roth: potential interest for wheat improvement. - Crop Sci. 41: 1321-1329, 2001. Go to original source...
    41. Zhang, H., Jia, J., Gale, M.D., Devos, K.M.: Relationships between the chromosomes of Aegilops umbellulata and wheat. - Theor. appl. Genet. 96: 69-75, 1998. Go to original source...

    Return to the content