Biologia plantarum 54:373-376, 2010 | DOI: 10.1007/s10535-010-0067-2

Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber

W. -P. Diao1, S. -Y. Bao1, B. Jiang1, L. Cui1, C. -T. Qian1, J. -F. Chen1,*
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Southern Vegetable Crop Genetic Improvement, Nanjing Agricultural University, Nanjing, P.R. China

With improved staining and chromosome preparation techniques, meiosis of pollen mother cells (PMCs) and male gametophyte development in autotetraploid cucumber (Cucumis sativus L.) was studied to understand the correlation between chromosomes behaviour and fertility. Various chromosome configurations, e.g. multivalent, quadrivalents, trivalents, bivalents and univalents were observed in most PMCs at metaphase I. Lagging chromosomes were frequently observed at anaphase in both meiotic divisions. In addition, chromosomes segregations were not synchronous and equal in some PMCs during anaphase II and telophase II. Dyads, triads, tetrads with micronuclei and polyads were observed at tetrad stage, and the frequencies of normal tetrad with four microcytes were only 55.4 %. The frequency of abnormal behaviour in each stage of meiosis was counted, and the average value was 37.2 %. The normal meiotic process could be accomplished to form the microspore tetrads via simultaneous cytokinesis. Most microspores could develop into fertile gametophytes with 2 cells and 3 germ pores through the following stages: single-nucleus early stage, single-nucleus late stage and 2-celled stage. The frequency of abnormalities was low during the process of male gametophyte development. The germination rate of pollen grains was 46.9 %. These results suggested that abnormal meiosis in PMCs was the reason for low pollen fertility in the autotetraploid cucumber.

Keywords: chromosome configurations; Cucumis sativus L.; microspore development
Subjects: autotetraploid; chromosome characteristics; cucumber; Cucumis sativus; male gametophyte development; meiotic abnormalities; microspore development

Received: August 6, 2008; Accepted: January 25, 2009; Published: June 1, 2010Show citation

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Diao, W.-P., Bao, S.-Y., Jiang, B., Cui, L., Qian, C.-T., & Chen, J.-F. (2010). Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber. Biologia plantarum, 54(2), 373-376. doi: 10.1007/s10535-010-0067-2.
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