Biologia plantarum 54:748-752, 2010 | DOI: 10.1007/s10535-010-0134-8

Direct plant regeneration from encapsulated nodal segments of Vitex negundo

N. Ahmad1, M. Anis1,2,*
1 Department of Botany, Aligarh Muslim University, Aligarh, India
2 College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia

An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na2-alginate with 100 mM CaCl2 has been found to be optimum concentration for the production of uniform synthetic seed. For germination, the synseeds were cultured on Murashige and Skoog (MS) basal medium supplemented with kinetin (KIN) and α-naphthalene acetic acid (NAA) either singly or in various combinations. MS medium containing 2.5 μM KIN in combination with 1.0 μM NAA was found to be the optimum for maximum (92.6 ± 3.71 %) plantlet conversion frequency. Well developed regenerated plantlets were hardened, acclimatized and established in field, where they grew well without any detectable variation.

Keywords: cold storage, growth regulators; micropropagation; synthetic seed
Subjects: auxins; carbonic anhydrase; carotenoids; chlorophyll a,b; cytokinins; germination; in vitro culture, direct organogenesis; in vitro culture, rooting; photosynthetic rate; synthetic seeds; Vitex negundo

Received: April 14, 2009; Accepted: October 13, 2009; Published: December 1, 2010Show citation

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Ahmad, N., & Anis, M. (2010). Direct plant regeneration from encapsulated nodal segments of Vitex negundo. Biologia plantarum, 54(4), 748-752. doi: 10.1007/s10535-010-0134-8.
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