Biologia plantarum 2010, 54:789-792 | DOI: 10.1007/s10535-010-0144-6

Two enzymatic sources of nitric oxide in different organs of apple plant

H. J. Gao1,*, H. Q. Yang2,3, J. Y. Wang1, J. X. Wang1,*
1 Shandong Institute of Pomology, Taian, P.R. China
2 Shandong Agricultural University, Taian, P.R. China
3 State Key Laboratory of Crop Biology, Taian, P.R. China

Nitric oxide production, nitric oxide synthase (NOS) and mitochondrial nitrite-reducing activities in roots, leaves and stems of different developmental stages were investigated, using potted 3-year-old apple (Malus domestica Borkh.) trees. The arginine-dependent NOS activity is sensitive to NOS inhibitor L-NAME and aminoguanidine (AG), with L-NAME being more effective than AG. Endogenous NO production, NOS and mitochondrial nitrite-reducing activities are predominately presented in young leaves and especially in young white roots and young stems. Root and stem mitochondria can reduce nitrite to nitric oxide at the expense of NADH, however, this mitochondrial nitrite-reducing activity is absent in leaves.

Keywords: chemiluminescence; L-arginine; mitochondria; nitric oxide synthase; nitrite
Subjects: apple; Malus domestica; nitric oxide; nitric oxid synthase

Received: August 10, 2009; Accepted: April 5, 2010; Published: December 1, 2010Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Gao, H.J., Yang, H.Q., Wang, J.Y., & Wang, J.X. (2010). Two enzymatic sources of nitric oxide in different organs of apple plant. Biologia plantarum, 54(4), 789-792. doi: 10.1007/s10535-010-0144-6.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Beligni, M.V., Lamattina, L.: Nitric oxide: a non-traditional regulator of plant growth. - Trends Plant Sci. 6: 508-509, 2001. Go to original source...
    2. Besson-Bard, A., Pugin, A., Wendehenne, D.: New insights into nitric oxide signaling in plants. - Annu. Rev. Plant Biol. 59: 21-39, 2008. Go to original source...
    3. Bethke, P.C., Badger, M.R., Jones, R.L.: Apoplastic synthesis of nitric oxide by plant tissues. - Plant Cell 16: 332-341, 2004. Go to original source...
    4. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    5. Capone, R., Tiwari, B.S., Levine, A.: Rapid transmission of oxidative and nitrosative stress signals from roots to shoots in Arabidopsis. - Plant Physiol. Biochem. 42: 425-428, 2004. Go to original source...
    6. Chen, Z., Foster, M.W., Zhang, J., Mao, L., Rockman, H.A., Kawamoto, T., Kitagawa, K., Nakayama, K.I., Hess, D.T., Stamler, J.S.: An essential role for mitochondrial aldehyde dehydrogenase in nitroglycerin bioactivation. - Proc. nat. Acad. Sci. USA 102: 12159-12164, 2005. Go to original source...
    7. Corpas, F.J., Barroso, J.B., Carreras, A., Quirós, M., León, A.M., Romero-Puertas, M.C., Esteban, F.J., Valderrama, R., Palma, J.M., Sandalio, L.M., Gómez, M., Del Río, L.A.: Cellular and subcellular localization of endogenous nitric oxide in young and senescent pea plants. - Plant Physiol. 136: 2722-2733, 2004. Go to original source...
    8. Corpas, F.J., Barroso, J.B., Carreras, A., Valderrama, R., Palma, J.M., León, A.M., Sandalio, L.M., Del Río, L.A.: Constitutive arginine-dependent nitric oxide synthase activity in different organs of pea seedlings during plant development. - Planta 224: 246-254, 2006. Go to original source...
    9. Correa-Aragunde, N., Lombardo, C., Lamattina, L.: Nitric oxide: an active nitrogen molecule that modulates cellulose synthesis in tomato roots. - New Phytol. 179: 386-396, 2008. Go to original source...
    10. Crawford, N.M., Guo, F.Q.: New insights into nitric oxide metabolism and regulatory functions. - Trends Plant Sci. 10: 195-200, 2005. Go to original source...
    11. Del Río, L.A., Corpas, F.J., Barroso, J.B.: Nitric oxide and nitric oxide synthase activity in plants. - Phytochemistry 65: 783-792, 2004. Go to original source...
    12. Gabaldón, C., Gómez Ros, L.V., Pedreño, M.A., Ros Barceló, A.: Nitric oxide production by the differentiating xylem of Zinnia elegans. - New Phytol. 165: 121-130, 2005. Go to original source...
    13. Gao, H.J., Yang, H.Q., Du, F.L., Zhao, H.Z.: [Changes in arginine and nitric oxide levels in Malus hupehensis Rehd. seedlings during plant development.] - Plant Nutr. Fert. Sci. 14: 774-778, 2008. [In Chin.]
    14. Gao, H.J., Yang, H.Q., Wang, J.X.: Arginine metabolism in roots and leaves of apple (Malus domestica Borkh.): the tissue-specific formation of both nitric oxide and polyamines. - Sci. Hort. 119: 147-152, 2009. Go to original source...
    15. Gaupels, F., Furch, A.C.U., Will, T., Mur, L.A.J., Kogel, K.H., van Bel, A.J.E.: Nitric oxide generation in Vicia faba phloem cells reveals them to be sensitive detectors as well as possible systemic transducers of stress signals. - New Phytol. 178: 634-646, 2008. Go to original source...
    16. Grün, S., Lindermayr, C., Sell, S., Durner, J.: Nitric oxide and gene regulation in plants. - J. exp. Bot. 57: 507-516, 2006. Go to original source...
    17. Gupta, K.J., Stoimenova, M., Kaiser, W.M.: In higher plants, only root mitochondria, but not leaf mitochondria reduce nitrite to NO, in vitro and in situ. - J. exp. Bot. 56: 2601-2609, 2005. Go to original source...
    18. Han, X., Yang, H., Duan, K., Zhang, X., Zhao, H., You, S., Jiang, Q.: Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets. - Plant Cell Tissue Organ Cult. 96: 29-34, 2009. Go to original source...
    19. Kaiser, W.M., Gupta, K.J., Planchet, E.: Higher plant mitochondria as a source for NO. - In: Lamattina, L., Polacco, J.C. (ed.): Nitric Oxide in Plant Growth, Development and Stress Physiology. Pp. 1-14. Springer-Verlag, Berlin - Heidelberg 2007.
    20. Klepper, L., Hageman R.H.: The occurrence of nitrate reductase in apple leaves. - Plant Physiol. 44: 110-114, 1969. Go to original source...
    21. Neill, S.J., Desikan, R., Hancock, J.T.: Nitric oxide signalling in plants. - New Phytol. 159: 11-35, 2003. Go to original source...
    22. Planchet, E., Gupta K.J., Sonoda, M., Kaiser, W.M.: Nitric oxide emission from tobacco leaves and cell suspensions: rate-limiting factors and evidence for the involvement of mitochondrial electron transport. - Plant J. 41: 732-743, 2005. Go to original source...
    23. Salgado, I., Modolo, L.V., Augusto, O., Braga, M.R., Oliveira, H.C.: Mitochondrial nitric oxide synthesis during plant-pathogen interactions: role of nitrate reductase in providing substrates. - In: Lamattina, L., Polacco, J.C. (ed.): Nitric Oxide in Plant Growth, Development and Stress Physiology. Pp. 239-254. Springer-Verlag, Berlin - Heidelberg 2007.
    24. Stöhr, C.: Nitric oxide - a product of plant nitrogen metabolism. - In: Lamattina, L., Polacco, J.C. (ed.): Nitric Oxide in Plant Growth, Development and Stress Physiology. Pp. 15-34. Springer-Verlag, Berlin - Heidelberg 2007.
    25. Stöhr, C., Stremlau, S.: Formation and possible roles of nitric oxide in plant roots. - J. exp. Bot. 57: 463-470, 2006. Go to original source...
    26. Stöhr, C., Strube, F., Marx, G., Ullrich. W.R., Rockel, P.: A plasma membrane-bound enzyme of tobacco roots catalyses the formation of nitric oxide from nitrite. - Planta 212: 835-841, 2001.
    27. Stöhr, C., Ullrich, W.R.: Generation and possible roles of NO in plant roots and their apoplastic space. - J. exp. Bot. 53: 2293-2303, 2002. Go to original source...
    28. Wells, C.E., Eissenstat, D.M.: Beyond the roots of young seedlings: the influence of age and order on fine root physiology. - J. Plant Growth Regul. 21: 324-334, 2003.
    29. Wendehenne, D., Pugin, A., Klessig, D.F., Durner, J.: Nitric oxide: comparative synthesis and signaling in animal and plant cells. - Trends Plant Sci. 6: 177-183, 2001. Go to original source...
    30. Wilson, I.D., Neill, S.J., Hancock, J.T.: Nitric oxide synthesis and signalling in plants. - Plant Cell Environ. 31: 622-631, 2008. Go to original source...
    31. Xu, J., Yin, H.X., Liu, X.J., Yuan, T., Mi, Q., Yang, L.L., Xie, Z.X., Wang, W.Y.: Nitric oxide alleviates Fe deficiency-induced stress in Solanum nigrum. - Biol. Plant. 53: 784-788, 2009. Go to original source...
    32. Yamasaki, H., Cohen, M.F.: NO signal at the crossroads: polyamine-induced nitric oxide synthesis in plants? - Trends Plant Sci. 11: 522-524, 2006. Go to original source...

    Return to the content