Biologia plantarum 56:177-180, 2012 | DOI: 10.1007/s10535-012-0038-x

Synergistic effect of trimethoprim and bavistin for micropropagation of Bacopa monniera

K. N. Tiwari1,*, V. Tiwari2, J. Singh1, B. D. Singh3, P. Ahuja3
1 Department of Botany, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, India
2 Department of Basic Medical Sciences, College of Osteopathic Medicine of The Pacific, Western University of Health Sciences, Pomona, USA
3 School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India

A micropropagation protocol for Bacopa monniera (L.) Wettst., a medicinally important plant, has been developed. Direct organogenesis without callus formation was induced by culturing node, internode and leaf explants on growth regulator free Murashige and Skoog (MS) medium. MS medium supplemented with an antibiotic trimethoprim (TMP) and a fungicide bavistin (BVN) produced axillary shoots from node and adventitious shoot buds on the surface of all explants. The combination of 200 mg dm-3 TMP and 200 mg dm-3 BVN induced the optimum frequency of shoot formation as well as shoot number. Presence of both TMP and BVN induced multiple axillary shoot formation from the nodal segments and this ability was maintained for four subcultures.

Keywords: adventitious shoot bud; axillary shoot
Subjects: adventitious shoot bud; axillary shoot; trimethoprim; bavistin; in vitro culture; benzyladenine; indoleacetic acid; indolebutyric acid
Species: Bacopa monniera

Received: October 13, 2009; Accepted: March 7, 2011; Published: March 1, 2012Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Tiwari, K.N., Tiwari, V., Singh, J., Singh, B.D., & Ahuja, P. (2012). Synergistic effect of trimethoprim and bavistin for micropropagation of Bacopa monniera. Biologia plantarum, 56(1), 177-180. doi: 10.1007/s10535-012-0038-x.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Aoshima, Y.: Efficient embryogenesis in the callus of tea (Camellia sinensis) enhanced by the osmotic stress or antibiotics treatment. - Plant Biotechnol. 22: 277-280, 2005. Go to original source...
    2. Bhattacharjee, B., Mohan, M., Nair, S.: Transformation of chickpea: effect of genotype, explant, Agrobacterium-strain and composition of culture medium. - Biol. Plant. 54: 21-32, 2010. Go to original source...
    3. Burgos, L., Alburquerque, N.: Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves. - Plant Cell Rep. 21: 1167-1174, 2003. Go to original source...
    4. Dibax, R., Deschamps, C., Bespalhok Filho, J.C., Vieira, L.G.E., Molinari, H.B.C., De Campos, M.K.F., Quoirin, M.: Organogenesis and Agrobacterium tumefaciens-mediated transformation of Eucalyptus saligna with P5CS gene. - Biol. Plant. 54: 6-12, 2010. Go to original source...
    5. Gomez, K.A., Gomez, A.A.: Statistical Procedures for Agricultural Research. - John Wiley & Sons, New York 1984.
    6. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    7. Rastogi, S., Pla, R.., Kulshreshtha, D.K.: Bacoside A3-A triterpenoid saponin from Bacopa monniera. - Phytochemistry 36: 133-137, 1994. Go to original source...
    8. Roodenrys, S., Booth, D., Bulzomi, S., Phipps, A., Micallef, C., Smoker, J.: Chronic effect of brahami (Bacopa monniera) on human memory. - Neuropsychopharmacology 27: 279-81, 2002. Go to original source...
    9. Tefera, W., Wannakrairoj, S.: Synergistic effects of some plant growth regulators on in vitro shoot proliferation of korarima (Aframomum corrorima (Braun) Jansen). - Afr. J. Biotechnol. 5: 1894-1901, 2006.
    10. Thomas, T.H.: Investigations into the cytokinin-like properties of benzimidazole derived fungicides. - Ann. appl. Biol. 76: 237-241, 1974. Go to original source...
    11. Tripathi, R.K., Ram, S.: Induction of growth and differentiation of carrot callus cultures by carbendazim and benzimidazole. - Indian J. exp. Biol. 20: 674-677, 1982.
    12. Tiwari, V., Singh, B.D., Tiwari, K.N.: Shoot regeneration and somatic embryogenesis from different explants of brahmi [Bacopa monniera (L.) Wettst.]. - Plant Cell Rep. 17: 538-543, 1998. Go to original source...
    13. Tiwari, V., Tiwari, K.N., Singh, B.D.: Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. - Plant Cell Tissue Organ Cult. 66: 9-16, 2001. Go to original source...
    14. Tiwari, V., Tiwari, K.N., Singh, B.D.: Shoot bud regeneration from different explants of Bacopa monniera (L.) Wettst. by trimethoprim and bavisitin. - Plant Cell Rep. 25: 629-635, 2006. Go to original source...
    15. Werbrouck, S.P.O., Debergh, P.C.: Imazalil enhances the shoot inducing effect of benzyladenine in Spathiphyllum floribundum Schott. - J. Plant Growth Regul. 14: 1905-107, 1995. Go to original source...
    16. Werbrouck, S.P.O., Debergh, P.C.: Imidazol fungicides and paclobutrazol enhance cytokinin-induced adventitious shoot proliferation in Araceae. - J. Plant Growth Regul. 15: 81-85, 1996. Go to original source...
    17. Werbrouck, S.P.O., Debergh, P.C.: Possible role of gibberellins in the interaction between cytokinins and pesticides. - Acta Hort. 447: 59-62, 1997. Go to original source...

    Return to the content