Biologia plantarum 2013, 57:85-90 | DOI: 10.1007/s10535-012-0258-0

GmPOI gene encoding a Pollen_Ole_e_I conserved domain is involved in response of soybean to various stresses

W. W. Song1, F. M. Duan1, W. B. Li2, Q. Lin1, H. X. Zhou1, X. Han3, J. A. Wang2,*
1 College of Crop Protection and Agronomy, Qingdao Agricultural University, Qingdao, P.R. China
2 College of Agronomy, Northeast Agricultural University, Harbin, P.R. China
3 The Crop Research and Breeding Center of Heilongjiang Land-Reclamation, Harbin, P.R. China

In the previous research, a novel gene GmPOI (GenBank acc. No. HM235775) encoding a Pollen_Ole_e_I conserved domain was identified in roots of soybean drought resistant cv. Jindou 23. In the present study, GmPOI was cloned and functionally characterized. Real-time quantitative PCR indicated that the expression of GmPOI was induced by drought, cold, salt and abscisic acid in wild-type soybean. The soybean plants overexpressing GmPOI showed higher tolerance to drought stress than wild types. We concluded that GmPOI is probably a novel gene that is involved in the response to various stresses in soybean.

Keywords: drought resistance; Glycine max; real-time PCR
Subjects: tolerance to abiotic stresses; soybean; drought resistance; water stress; transgenic plants; salinity; abscisic acid; temperature low
Species: Glycine max

Received: December 14, 2011; Accepted: April 11, 2012; Published: March 1, 2013Show citation

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Song, W.W., Duan, F.M., Li, W.B., Lin, Q., Zhou, H.X., Han, X., & Wang, J.A. (2013). GmPOI gene encoding a Pollen_Ole_e_I conserved domain is involved in response of soybean to various stresses. Biologia plantarum, 57(1), 85-90. doi: 10.1007/s10535-012-0258-0.
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    References

    1. Alché, J.D., Castro, A.J., Jiménez-López, J.C., Morales, S., Zafra, A., Hamman-Khalifa, A.M., Rodríguez-García, M.I.: Differential characteristics of olive pollen from different cultivars: biological and clinical implications. - J. Investig. Allergol. clinical Immunol. 17(Suppl.): 69-75, 2007.
    2. Askari, H., Edqvist, J., Hajheidari, M., Kafi, M., Salekdeh, G.H.: Effects of salinity levels on proteome of Suaeda aegyptiaca leaves. - Proteomics 6: 2542-2554, 2006. Go to original source...
    3. Astwood, J.D., Hill, R.D.: Cloning and expression pattern of Hor v 9, the group 9 pollen isoallergen from barley. - Gene 182: 53-62, 1996. Go to original source...
    4. Barral, P., Suárez, C., Batanero, E., Alfonso, C., Alché, J.D., Rodríguez-García, M.I., Villalba, M., Rivas, G., Rodríguez, R.: An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination. - Biochem. J. 390: 77-84, 2005. Go to original source...
    5. Batanero, E., González de la Pena, M.A., Villalba, M., Monsalve, R.I., Martín-Esteban, M., Rodríguez, R.: Isolation, cDNA cloning and expression of Lig v 1, the major allergen from privet pollen. - Clinical exp. Allergy 26: 1401-1410, 1996. Go to original source...
    6. Batanero, E., Villalba, M., Rodríguez, R.: Glycosylation site of the major allergen from olive tree pollen. - Allergenic implications of the carbohydrate moiety. - Mol. Immunol. 31: 31-37, 1994. Go to original source...
    7. Bateman, A., Coin, L., Durbin, R., Finn, R.D., Hollich, V., Griffiths-Jones, S., Khanna, A., Marshall, M., Moxon, S., Sonnhammer, E.L.L., Studholme, D.J., Yeats, C., Eddy, S.R.: The Pfam protein families database. - Nucl. Acids Res. 32: 138-141, 2004. Go to original source...
    8. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil. 39: 205-207, 1973. Go to original source...
    9. Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. - Anal. Biochem. 44: 276-286, 1971. Go to original source...
    10. Borsani, O., Valpuesta, V., Botella, M.A.: Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. - Plant Physiol. 126: 1024-1030, 2001. Go to original source...
    11. Bray, E.A.: Plant responses to water deficit. - Trends Plant Sci. 2: 48-54, 1997. Go to original source...
    12. Carpenter, J.L., Ploense, S.E., Snuatad, D.P., Silflow, C.D.: Preferential expression of an [alpha]-Tubulin gene of Arabidopsis in pollen. - Plant Cell. 4: 557-571, 1992. Go to original source...
    13. Grobei, M.A., Qeli, E., Brunner, E.: Deterministic protein inference for shotgun proteomics data provides new insights into Arabidopsis pollen development and function. - Genome Res. 19: 1786-1800, 2009.
    14. Gutierrez-Gonzalez, J.J., Guttikonda, S.K., Tran, L.S., Aldrich, D.L., Zhong, R., Yu, O., Nguyen, H.T., Sleper, D.A.: Differential expression of isoflavone biosynthetic genes in soybean during water deficits. - Plant Cell Physiol. 51: 936-948, 2010. Go to original source...
    15. Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J.: Plant cellular and molecular responses to high salinity. - Annu. Rev. Plant Physiol. Plant mol Biol. 51: 463-499, 2000. Go to original source...
    16. Hirayama, T., Shinozaki, K.: Perception and transduction of abscisic acid signals, keys to the function of the versatile plant hormone ABA. - Trends Plant Sci. 12: 343-351, 2007. Go to original source...
    17. Jiang, S.Y., Cai, M., Ramachandran, S.: The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domaincontaining protein. - Plant mol. Biol. 57: 835-853, 2005. Go to original source...
    18. Jiménez-López, J.C., Rodríguez-García, M.I., Alché, J.D.: Systematic and phylogenetic analysis of the Ole e 1 pollen protein family members in plants. - In: Yang, N.S. (ed): Systems and Computational Biology - Bioinformatics and Computational Modeling. Pp. 245-260. InTech Press, Granada 2011.
    19. Kandasamy, M.K., Mckinney, E.C., Meagher, R.B.: The late pollen-specific actins in angiosperms. - Plant J. 18: 681-691, 1999. Go to original source...
    20. Manavalan, L.P., Guttikonda, S.K., Tran, L.S., Nguyen, H.T.: Physiological and molecular approaches to improve drought resistance in soybean. - Plant Cell Physiol. 50: 1260-1276, 2009. Go to original source...
    21. Mitsuda, N., Takeyasu, K., Sato, M.H.: Pollen-specific regulation of vacuolar HC-PPase expression by multiple cisacting elements. - Plant mol. Biol. 46: 185-192, 2001. Go to original source...
    22. Muschietti, J., Dircks, L., Vancanneyt, G., McCormick, S.: LAT52 protein is essential for tomato pollen development: pollen expressing antisense LAT52 RNA hydrates and germinates abnormally and cannot achieve fertilization. - Plant J. 6: 321-338, 1994. Go to original source...
    23. Okada, T., Zhang, Z., Russell, S.D., Toriyama, K.: Localization of the Ca2+-binding protein, Bra r 1, in anthers and pollen tubes. - Plant Cell Physiol. 40: 1243-1252, 1999. Go to original source...
    24. Qiao, H., Wang, F., Zhao, L., Zhou, J.L., Lai, Z., Zhang, Y.S., Robbins, T.P., Xue, Y.B.: The F-Box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility. - Plant Cell. 16: 2307-2322, 2004. Go to original source...
    25. Rabello, A.R., Guimarães, C.M., Rangel, P.H.N., Da Silva, F.R., Seixas, D., De Souza, E., Brasileiro, A.C.M., Spehar, C.R., Ferreira, M.E., Mehta, Â.: Identification of droughtresponsive genes in roots of upland rice (Oryza sativa L). - BMC Genomics 9: 485, 2008. Go to original source...
    26. Rodríguez, R., Villalba, M., Monsalve, R.I., Batanero, E., González, E.M., Monsalve, R.I., Huecas, S., Tejera, M.L., Ledesma, A.: Allergenic diversity of the olive pollen. - Allergy 57: 6-15, 2002a. Go to original source...
    27. Stratford, S., Barne, W., Hohorst, D.L., Sagert, J.G., Cotter, R., Golubiewski, A., Showalter, A.M., McCormick, S., Bedinger, P.: A leucine-rich repeat region is conserved in pollen extensin-like (Pex) proteins in monocots and dicots. - Plant mol Biol. 46: 43-56, 2001. Go to original source...
    28. Thangavelu, M., Belostosky, D., Bevan, M.W., Flavell, R.B., Rogers, H.J., Lonsdale, D.M.: Partial characterization of the Nicotiana tobacum action gene family; evidence for pollenspecific expression of one of the gene family member. - Mol. gen. Genet. 240: 290-295, 1993. Go to original source...
    29. Thapa, G., Dey, M., Sahoo, L., Panda, S.K.: An insight into drought stress induced alterations in plants. - Biol. Plant. 55: 603-613, 2011. Go to original source...
    30. Tran, L.S., Nguyen, H.T.: Future biotechnology of legumes. - In: Emerich, W.D., Krishnan, H. (ed.): Nitrogen Fixation in Crop Production. Pp. 265-308. ASA, CSA, SSSA, Madison 2009.
    31. Twell, D., Wing, R.A., Ushiba, J., McCormick, S.: Promote analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements. - Genes Dev. 5: 496-507, 1991. Go to original source...
    32. Twell, D., Yamaguchi, J., McCormick, S.: Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. - Development. 109: 705-713, 1990.
    33. Verbruggen, N., Hermans, C.: Proline accumulation in plants: a review. - Amino Acids 35: 753-759, 2008. Go to original source...
    34. Weterings, K., Schrauwen, J., Wullurms, G., Twell, D.: Functional dissection of the promoter of the pollen-specific gene NTP303 reveals a novel pollen-specific and conserved cis-regulatory element. - Plant J. 8: 55-63, 1995. Go to original source...
    35. Yu, L.X., Nasrllah, J., Valenta, R., Parthasarthy, M.V.: Molecular cloning and mRNA localization of tomato pollen profiling. - Plant mol. Biol. 36: 699-707, 1998. Go to original source...
    36. Zachgo, S., Sacdler, H., Schwarz-Sommer, Z.: Pollen-specific expression of DEFH125 a MADS-box transcription factor in Antirrhinum with unusual features. - Plant J. 11: 1043-1050, 1997. Go to original source...
    37. Zhu, J.K.: Salt and drought stress signal transduction in plants. - Annu. Rev. Plant Biol. 53: 247-273, 2002. Go to original source...

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