Biologia plantarum 57:663-674, 2013 | DOI: 10.1007/s10535-013-0332-2

Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping

U. Lohwasser1,*, M. A. Rehman Arif1, A. Börner1
1 Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

Three wheat and two barley populations were studied in order to find loci responsible for dormancy and pre-harvest sprouting. A classical quantitative trait loci analysis was combined with an association mapping approach. Many quantitative trait loci and marker trait associations could be detected on all seven chromosome groups of wheat and on the chromosomes 2H, 3H, 5H, 6H, and 7H of barley. Especially, the known regions on chromosomes 3A and 4A for wheat and 5H for barley were confirmed. Putative functions could be found via a candidate homologues search and via expressed sequence tag annotation. On chromosome 3A, the viviparous1 gene is located which is associated to preharvest sprouting and dormancy. On chromosome 4A, a protein is detected which belongs to the aquaporin family. In barley, an association with the aleurain gene on chromosome 5H was found. The expression of aleurain is regulated by abscisic acid and gibberelic acid. An influence of both hormones on dormancy and pre-harvest sprouting is known. It can be concluded that dormancy and pre-harvest sprouting are very complex traits regulated by multigenes and/or quantitative trait loci.

Keywords: abscisic acid; gene function; gibberellic acid; Hordeum vulgare; quantitative trait loci; Triticum aestivum
Subjects: pre-harvest sprouting; dormancy; abscisic acid; gibberellic acid; quantitative trait loci; chromosomes; association mapping; barley; wheat
Species: Hordeum vulgare; Triticum aestivum

Received: August 28, 2012; Accepted: February 11, 2013; Published: December 1, 2013Show citation

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Lohwasser, U., Arif, M.A., & Börner, A. (2013). Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping. Biologia plantarum, 57(4), 663-674. doi: 10.1007/s10535-013-0332-2.
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