Biologia plantarum 58:121-130, 2014 | DOI: 10.1007/s10535-013-0376-3

Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress

X. Meng1, B. Yin1, H. -L. Feng1, S. Zhang1, X. -Q. Liang1, Q. -W. Meng1,*
1 College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China

LeAN2 encoding an anthocyanin-associated R2R3-MYB transcription factor was isolated from tomato. The expression of LeAN2 in tomato was induced by low temperature and oxidative stress. Green fluorescent protein was fused to LeAN2 and the complex was expressed transiently in onion epidermal cells. Green fluorescence was observed only in the nucleus. Overexpression of LeAN2 under the control of the CaMV35S promoter in tobacco induced expression of several anthocyanin biosynthetic genes and the content of anthocyanin was markedly higher in transgenic tobacco compared with wild type plants. Transgenic tobaccos conferred tolerance to chilling stress by maintaining a higher chlorophyll content, net photosynthetic rate, and maximal photochemical efficiency of PS II compared to the wild type plants. Furthermore, the transgenic plants showed lower ion leakage, a lower content of reactive oxygen species, and a higher content of non-enzymatic antioxidans under the chilling stress. They also showed an enhanced resistance to the oxidative stress induced by methyl viologen based on a decreased chlorophyll content loss, lower ion leakage, and an enhanced maximal photochemical efficiency of PS II. These results indicate that overexpression of LeAN2 resulted in an increased anthocyanin accumulation and enhanced resistance to the chilling and oxidative stresses in transgenic tobacco.

Keywords: Allium cepa; chlorophyll; fluorescence; Lycopersicum esculentum; net photosynthetic rate; Nicotiana tabacum; onion; tomato; transcription factor; transgenic tobacco
Subjects: anthocyanin biosynthesis; anthocyanin biosynthesis-related genes; temperature - low; cold resistance; oxidative stress; methyl viologen; green fluorescent protein; transgenic plans; chlorophyll a fluorescence; net photosynthetic rate; chlorophyll content; tomato; tobacco; onion
Species: Allium cepa; Solanum lycopersicum; Nicotiana tabacum

Received: May 6, 2013; Revised: June 19, 2013; Accepted: June 24, 2013; Published: March 1, 2014Show citation

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Meng, X., Yin, B., Feng, H.-L., Zhang, S., Liang, X.-Q., & Meng, Q.-W. (2014). Overexpression of R2R3-MYB gene leads to accumulation of anthocyanin and enhanced resistance to chilling and oxidative stress. Biologia plantarum, 58(1), 121-130. doi: 10.1007/s10535-013-0376-3.
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