Biologia plantarum 58:169-173, 2014 | DOI: 10.1007/s10535-013-0366-5

Effects of exogenous hydrogen sulfide on the redox states of ascorbate and glutathione in maize leaves under salt stress

C. Shan1,*, H. Liu2, L. Zhao3, X. Wang4
1 Henan Institute of Science and Technology, Xinxiang, P.R. China
2 Xinxiang Medical University, Xinxiang, P.R. China
3 College of Life Sciences, Shangqiu Normal University, Shangqiu, P.R. China
4 College of Life Science, Henan Normal University, Xinxiang, P.R. China

This study investigated the effects of exogenous hydrogen sulfide (H2S) on the redox states of ascorbate (AsA) and glutathione (GSH) in maize leaves under NaCl (100 mM) stress. Salt stress increased the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), Γ-glutamylcysteine synthetase (Γ-ECS), and L-galactono-1,4-lactone dehydrogenase (GalLDH), malondialdehyde content and electrolyte leakage, and reduced the ratios of reduced and oxidised forms of AsA (AsA/DHA) and GSH (GSH/GSSG) compared with control. Pretreatment with NaHS (H2S donor) further enhanced the activities of the above enzymes except MDHAR and ameliorated the decrease in the ratios of AsA/DHA and GSH/GSSG compared with the salt stress alone. Pretreatment with NaHS significantly reduced the malondialdehyde content and electrolyte leakage induced by the salt stress. Pretreatment with NaHS alone did not affect any of the above mentioned parameters compared with the control. Our results suggest that exogenous H2S could maintain the redox states of ascorbate and glutathione by up-regulating the ascorbate and glutathione metabolism and thus play an important role for acquisition of salt stress tolerance in maize.

Keywords: ascorbate peroxidase; electrolyte leakage; glutathione reductase; malondialdehyde; NaCl; Zea mays
Subjects: hydrogen sulfide; ascorbate; glutathione; salinity; ascorbate peroxidase; glutathione reductase; electrolyte leakage; malondialdehyde; maize

Received: December 14, 2012; Revised: April 15, 2013; Accepted: May 28, 2013; Published: March 1, 2014Show citation

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Shan, C., Liu, H., Zhao, L., & Wang, X. (2014). Effects of exogenous hydrogen sulfide on the redox states of ascorbate and glutathione in maize leaves under salt stress. Biologia plantarum, 58(1), 169-173. doi: 10.1007/s10535-013-0366-5.
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