Biologia plantarum 61:651-658, 2017 | DOI: 10.1007/s10535-017-0718-7

Transcription factor NnDREB1 from lotus improved drought tolerance in transgenic Arabidopsis thaliana

L. B. Cheng1, J. J. Yang1, L. Yin1, L. C. Hui1, H. M. Qian1, S. -Y. Li2,*, L. -J. Li1,*
1 School of Horticulture and Plant Protection, Yangzhou University, Jiangsu, P.R. China
2 College of Guangling, Yangzhou University, Jiangsu, Jiangsu, P.R. China

Dehydration responsive element binding factor (DREB) is believed to be a stress-tolerance enhancer in plants. In the present study, a cold-binding factor (CBF)/DREB homologous gene NnDREB1 (XP_010242642.1) was isolated from lotus roots using rapid amplification of cDNA ends (RACE) and reverse transcription (RT)-PCR methods. Analysis of the deduced amino acid sequence and phylogeny classified NnDREB1 into the A-1 group of the DREB1 subfamily. Expression profiling using a quantitative PCR method revealed that NnRDEB1 was significantly induced by NaCl, mannitol, and polyethylene glycol, but not by low temperature and abscisic acid. To evaluate function of NnRDEB1, Arabidopsis thaliana was transformed with the NnDREB1 gene in a binary vector construct. The transgenic plants exhibited higher resistance to drought compared with the wild-type plants in terms of survival rates, dry and fresh masses, and chlorophyll content. In addition, overexpression of NnDREB1 resulted in higher germination rates compared with the wild type plants on MS medium containing mannitol. The expressions of downstream target stressrelated genes, including cold-regulated15B (COR15B), rare cold inducible 2B (RCI2B) and repeat domain 26 (RD26), were activated in the transgenic plants. Taken together, the results suggest that NnDREB1 might be an important protein in lotus root drought tolerance.

Keywords: chlorophyll; gene expression; mannitol; Nelumbo nucifera; phylogenetic tree; polyethylene glycol; salinity
Subjects: transcription factor; DREB; drought tolerance; transgenic plants; chlorophyll; mannitol; polyethylene glycol; salinity; phylogenetic tree; abscisic acid; cold stress; lotus
Species: Nelumbo nucifera; Arabidopsis thaliana

Received: July 25, 2015; Revised: December 15, 2016; Accepted: January 2, 2017; Published: December 1, 2017Show citation

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Cheng, L.B., Yang, J.J., Yin, L., Hui, L.C., Qian, H.M., Li, S.-Y., & Li, L.-J. (2017). Transcription factor NnDREB1 from lotus improved drought tolerance in transgenic Arabidopsis thaliana. Biologia plantarum, 61(4), 651-658. doi: 10.1007/s10535-017-0718-7.
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    References

    1. Agarwal, P.K., Agarwal, P., Reddy, M.K., Sopory, S.K.: Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. - Plant Cell Rep. 25: 1263-1274, 2006. Go to original source...
    2. Bhargava, S., Sawant, K.: Drought stress adaptation: metabolic adjustment and regulation of gene expression. - Plant Breed. 132: 21-32, 2013. Go to original source...
    3. Borgi, W., Ghedira, K., Chouchane, N.: Antiinflammatory and analgesic activities of Zizyphus lotus root barks. - Fitoterapia 78: 16-19, 2007. Go to original source...
    4. Charfeddine, M., Bouaziz, D., Charfeddine, S., Hammami, A., Ellouz, O. N., Bouzid, R.G.: Overexpression of dehydration-responsive element-binding 1 protein (DREB1) in transgenic Solanum tuberosum enhances tolerance to biotic stress. - Plant Biotechnol. Rep. 9: 79-88, 2015. Go to original source...
    5. Cheng, L.B., Li, S.Y., Yin, J.J., Li, L.J., Chen, X.H.: Genomewide analysis of differentially expressed genes relevant to rhizome formation in lotus root (Nelumbo nucifera Gaertn). - PLoS ONE 8: e67116, 2013. Go to original source...
    6. Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. - Plant J. 16: 735-743, 1998. Go to original source...
    7. Dubouzet, J.G., Sakuma, Y., Ito, Y., Kasuga, M., Dubouzet, E.G., Miura, S., Seki, M., Shinozaki, K., Yamaguchi-Shinozak, K.: OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. - Plant J. 33: 751-763, 2003.
    8. Fang, Z.W., Zhang, X.H., Gao, J.F., Wang, P.K., Xu, X.Y., Liu, Z.X., Shen, S.H., Feng, B.L.: A buckwheat (Fagopyrum esculentum) DRE-binding transcription factor gene, FeDREB1, enhances freezing and drought tolerance of transgenic Arabidopsis. - Plant mol. Biol. Rep. 33: 1510-1525, 2015. Go to original source...
    9. Fujita, M., Fujita, Y., Maruyama, K., Seki, M., Hiratsu, K., Ohme-Takagi, M., Tran, L.S.P., Yamaguchi-Shinozaki, K., Shinozaki, K.: A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. - Plant J. 39, 863-876, 2004. Go to original source...
    10. Giulietti, A., Overbergh, L., Valckx, D., Decallonne, B., Bouillon, R., Mathieu, C.: An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. - Methods 25, 386-401, 2001. Go to original source...
    11. Haake, V., Cook, D., Riechmann, J.L., Pineda, O., Thomashow, M.F., Zhang, J.Z.: Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. - Plant Physiol. 130: 639-648, 2002. Go to original source...
    12. Hiscox, J.D., Israelstam, G.F.: A method for extraction of cholorophyll from leaf tissue without maceration. - Can. J. Bot. 59: 463-469, 1979. Go to original source...
    13. Hsieh, T.H., Lee, J.T., Charng, Y.Y., Chan, M.T.: Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress. - Plant Physiol. 130: 618-626, 2002. Go to original source...
    14. Kim, Y.H., Yang, K.S., Ryu, S.H., Kim, K.Y., Song, W.K., Kwon, S.Y., Lee, H.S., Bang, J.W., Kwak, S.S.: Molecular characterization of a cDNA encoding DRE-binding transcription factor from dehydration-treated fibrous roots of sweet potato. - Plant Physiol. Biochem. 46: 196-204, 2008. Go to original source...
    15. Liu, J., Zhang, M., Wang, S.: Processing characteristics and flavour of full lotus root powder beverage. - J. Sci. Food Agr. 90: 2482-2489, 2010. Go to original source...
    16. Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K., Shinozaki, K.: Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis. - Plant Cell 10: 1391-1406, 1998. Go to original source...
    17. Magome, H., Yamaguchi, S., Hanada, A., Kamiya, Y.J., Oda, K.: The DDF1 transcriptional activator up-regulates expression of a gibberellin-deactivating gene, GA2ox7, under high-salinity stress in Arabidopsis. - Plant J. 56: 613-626, 2008. Go to original source...
    18. Manavalan, L.P., Guttikonda, S.K., Tran, L.S.P., Nguyen, H.T.: Physiological and molecular approaches to improve drought resistance in soybean. - Plant Cell Physiol. 50: 1260-1276, 2009. Go to original source...
    19. Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.: AP2/ERF family transcription factors in plant abiotic stress responses. - Biochim. biophys. Acta 1819: 86-96, 2012.
    20. Reis, RR., Da Cunha, B.A.D.B., Martins, P.K., Martins, M.T.B., Alekcevetch, J.C., Chalfun, A., Jr., Andrade, A.C., Ribeiro, A.P., Qin, F., Mizoi, J., Yamaguchi-Shinozaki, K., Nakashima, K., Carvalho, J.F.C., De Sousa, C.A.F., Nepomuceno, A.L., Kobayashi, A.K., Molinari, HBC.: Induced over-expression of AtDREB2A CA improves drought tolerance in sugarcane. - Plant Sci. 221-222: 59-68, 2014. Go to original source...
    21. Renato, B.R.A.Z., Hechenleitner, A.A.W., Cavalcanti, O.A.: Extraction, structural modification and characterization of lotus roots polysaccharides (Nelumbo nucifera Gaertn). Excipient with potential application in modified drug delivery systems. - Lat. Amer. J. Pharm. 26: 706-710, 2007.
    22. Sakuma, Y., Maruyama, K., Osakabe, Y., Qin, F, Seki, M., Shinozaki, K., Yamaguchi-Shinozaki, K.: Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought responsive gene expression. - Plant Cell 18: 1292-1309, 2006. Go to original source...
    23. Shinozaki, K., Yamaguchi-Shinozaki, K.: Gene networks involved in drought stress response and tolerance. - J. exp. Bot. 58: 221-227, 2007.
    24. Tang, M.J., Lu, S.Y., Jing, Y.X., Zhou, X.J., Sun, J.W., Shen, S.H.: Isolation and identification of a cold-inducible gene encoding a putative DRE-binding transcription factor from Festuca arundinacea. - Plant Physiol. Biochem. 43: 233-239, 2005. Go to original source...
    25. Tian, X.H., Li, X.P., Zhou, H.L., Zhang, J.S., Gong, Z.Z., Chen, S.Y.: OsDREB4 genes in rice encode AP2-containing proteins that bind specifically to the dehydration-responsive element. - J. Integr. Plant Biol. 47: 467-476, 2005. Go to original source...
    26. Tong, Z., Hong, B., Yang, Y.J., Li, Q.H., Ma, N.C., Gao, J.P.: Overexpression of two chrysanthemum DgDREB1 group genes causing delayed flowering or dwarfism in Arabidopsis. - Plant mol. Biol. 71: 115-129, 2009. Go to original source...
    27. Verlotta, A., Liberatore, M.T., Cattivelli, L., Trono, D.: Secretory phospholipases a2 in durum wheat (Triticum durum Desf.): gene expression, enzymatic activity, and relation to drought stress adaptation. - Int. J. mol. Sci. 14: 5146-5169, 2013. Go to original source...
    28. Wang, Q.Y., Guan, Y.C., Wu, Y.C., Chen, H.G., Chen, F., Chu, C.C.: Overexpression of a rice OsDREB1F gene increases salt, drought, and low temperature tolerance in both Arabidopsis and rice. - Plant mol. Biol. 67: 589-602, 2008. Go to original source...
    29. Wang, W.X., Vinocur, B., Shoseyov, O., Altman, A.: Biotechnology of plant osmotic stress tolerance: physiological and molecular consideration. - Acta Hort. 560: 285-292, 2001. Go to original source...
    30. Wery, J., Silim, S.N., Knights, E.J., Malhotra, R.S., Cousin, R.: Screening techniques and sources and tolerance to extremes of moisture and air temperature in cool season food legumes. - Euphytica 73: 73-83, 1994. Go to original source...
    31. Xiao, H., Siddiqua, M., Braybrook, S., Nassuth, A.: Three grape CBF/DREB1 genes respond to low temperature, drought and abscisic acid. - Plant Cell Environ. 29: 1410-1421, 2006. Go to original source...
    32. Yamaguchi-Shinozaki, K., Shinozaki, K.: A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low temperature, or high-salt stress. - Plant Cell 6: 251-264, 1994. Go to original source...
    33. Yang, W., Liu, X.D., Chi, X.J., Wu, C.A., Li, Y.Z., Song, L.L., Liu, X.M., Wang, Y.F., Wang, F.W., Zhang, C., Liu, Y., Zong, J.M., Li, H.Y.: Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. - Planta 233: 219-229, 2011. Go to original source...
    34. Zhao, J.S., Daying, W.R., Wang, L., Xia, G.G.: Arabidopsis DREB1A/CBF3 bestowed transgenic tall fescue increased tolerance to drought stress. - Plant Cell Rep. 26: 1521-1528, 2007. Go to original source...

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