Biologia plantarum 44:325-331, 2001 | DOI: 10.1023/A:1012470021514
Cytology of Potato Callus Cells in Relation to their Frost Hardiness
- 1 Department of Horticulture, University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan
Structure of callus cells of frost-sensitive and frost-tolerant Solanum species and a frost-tolerant cell line (D20-1), selected from S. tuberosum cv. Desirée callus, was studied. Like frost-tolerant species S. commersonii, cells of the frost-tolerant cell line contained starch grains in their plastids. The cells of this frost-tolerant line also possessed an increased number of microbodies containing protein crystals which suggests the involvement of proteins in frost tolerance but the mechanism may differ from that in frost-tolerant species.
Keywords: frost tolerance; microbodies; proteins; Solanum
Subjects: frost hardiness and tolerance, callus cell cytology; in vitro culture, frost tolerance; microbodies, frost hardiness, callus culture; potato; proteins, frost hardiness; Solanum acaule; Solanum commersonii; Solanum tuberosum
Published: September 1, 2001Show citation
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Anjum, M.A. (2001). Cytology of Potato Callus Cells in Relation to their Frost Hardiness. Biologia plantarum, 44(3), 325-331. doi: 10.1023/A:1012470021514.
References
- Anjum, M.A., Villiers, T.A.: Selection of hydroxyproline-resistant cell lines from Solanum tuberosum L. callus. I. Stability and frost tolerance.-J. Genet. Breed. 52: 113-117, 1998.
- Antikainen, M., Pihakaski, S.: Cold-induced changes in the polysome pattern and protein synthesis in winter ryc (Secale cereale) leaves.-Physiol Plant. 89: 111-116, 1993. Go to original source...
- Burgess, J.: An Introduction to Plant Cell Development.-Cambridge University Press, Cambridge 1985.
- Cabane, M., Vincens, P., Boudet, A.M.: Protein synthesis at low temperatures in two soybean cultivars differing in their cold sensitivity.-Physiol. Plant. 85: 573-580, 1992. Go to original source...
- Chen, H.H., Li, P.H.: Characteristics of cold acclimation and deacclimation in tuber-bearing Solanum species.-Plant Physiol. 65: 1146-1148, 1980. Go to original source...
- Chen, P., Li, P.H., Cunningham, W.P.: Ultrastructural differences in leaf cells of some Solanum species in relation to their frost hardiness.-Bot. Gaz. 138: 276-285, 1977. Go to original source...
- Handley, L.W., Sink, K.C.: Plant regeneration of protoplasts isolated from suspension cultures of Solanum lycopersicoides.-Plant Sci. 42: 201-207, 1985. Go to original source...
- Irzykowski, W., Rorat, T., Jakubiec, J.: Analysis of cold resistance in wild and cultivated potato species.-Acta Physiol. Plant. 18: 217-221, 1996.
- Juniper, B.E., Cox, G.C., Gilchrist, A.J., Williams, R.R.: Techniques for Plant Electron Microscopy.-Blackwell Scientific Publications, Oxford 1970.
- Mollenhauer, H.H.: Plastic embedding mixture for use in electron microscopy.-Stain Technol. 39: 111-114, 1964.
- Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-479, 1962. Go to original source...
- Pinedo, M.L., Hernández, G.F., Conde, R.D., Tognetti, J.A.: Effect of low temperature on the protein metabolism of wheat leaves.-Biol. Plant. 43: 363-367, 2000. Go to original source...
- Reynolds, E.S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy.-J. Cell Biol. 17: 208-213, 1963. Go to original source...
- Rorat, T., Irzykowski, W., Grygorowicz, W.J.: Identification and expression of novel cold induced genes in potato (Solanum sogarandinum).-Plant Sci. 124: 69-78, 1997. Go to original source...
- Stone, J.M., Palta, J.P., Bamberg, J.B., Weiss, L.S.: Freezing tolerance and cold acclimation capacity are genetically distinct quantitative traits in a segregating Solanum population.-Plant Physiol. 99(Suppl.): S-126, 1992.
- Stone, J.M., Palta, J.P., Bamberg, J.B., Weiss, L.S., Harbage, J.F.: Inheritance of freezing resistance in tuber-bearing Solanum species: Evidence for independent genetic control of non-acclimated freezing tolerance and cold acclimation capacity.-Proc. nat. Acad. Sci. USA 90: 7869-7873, 1993. Go to original source...
- Sutka, J., Worland, A.J., Maystrenko, O.I.: Slight effect of cytoplasm on frost resistance in wheat (Triticum oestivum L.).-Cereal Res. Commun. 19: 311-317, 1991.