Biologia plantarum 45:449-453, 2002 | DOI: 10.1023/A:1016238205522

Effective Protocol for in Vitro Shoot Production Through Nodal Explants of Simmondsia Chinensis

V. Agrawal1, S. Prakash1, S.C. Gupta1
1 Department of Botany, University of Delhi, Delhi, India

Nodal explants excised from 18 to 20-year-old female plants of Simmondsia chinensis if cultured on Murashige and Skoog's medium supplemented with 20 μM N6-benzyladenine (BA) differentiated an average of 2.7 ± 0.4 shoots in 11.5% explants. The percentage of nodal explants inducing multiple shoots enhanced significantly if in vitro raised shoots were used as source of explants. Nearly 100% cultures differentiated an average of 4.7 ± 2.0 shoots per explant on the same medium. Nearly 85% of the shoots induced roots when a pulse treatment of 50 μM indole-3-butyric acid (IBA) was given prior to their transfer to semi-solid MS medium containing 10 μM IBA + 0.5% activated charcoal + 1 μM BA. Plantlets were gradually hardened in Soilrite and acclimatized to soil.

Keywords: activated charcoal; benzyladenine; indole-3-butyric acid; jojoba; multiple shoots
Subjects: cytokinins; in vitro culture, plant regeneration, organogenesis; in vitro culture, shoot production from nodal explants; jojoba, multiple shoots; Simmondsia chinensis

Published: September 1, 2002Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Agrawal, V., Prakash, S., & Gupta, S.C. (2002). Effective Protocol for in Vitro Shoot Production Through Nodal Explants of Simmondsia Chinensis. Biologia plantarum, 45(3), 449-453. doi: 10.1023/A:1016238205522.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Agrawal, V., Gupta, S.C.: In vitro plantlet development from 25-year-old tree explants of Populus × euramericana - a hybrid poplar.-Plant Sci. 78: 99-105, 1991. Go to original source...
    2. Agrawal, V., Prakash, S., Gupta, S.C.: Differential hormonal requirements for male and female jojoba plants.-In: Altman, A., Izhar, S., Ziv, M. (ed.): Plant Biotechnology and in Vitro Biology in the 21st Century. Pp. 25-28. Kluwer Academic Publishers, Dordrecht 1999. Go to original source...
    3. Boggetti, B., Jásik, J., Mantell, S.H.: In vitro root formation in Anacardium occidentale microshoots.-Biol. Plant. 44: 175-179, 2001. Go to original source...
    4. Chaturvedi, H.C., Sharma, M.: In vitro production of cloned plants of jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long term culture.-Plant Sci. 63: 199-207, 1989. Go to original source...
    5. Fridborg, G., Pedersen, M., Landstrom, M., Eriksson, T.: The effect of activated charcoal on tissue cultures: absorption of metabolites inhibiting morphogenesis.-Physiol. Plant. 43: 104-106, 1978. Go to original source...
    6. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root callus.-Exp. Cell Res. 50: 15-158, 1968. Go to original source...
    7. Gentry, H.S.: The natural history of jojoba (Simmondsia chinensis) and its cultural aspects.-Econ. Bot. 12: 261-295, 1958. Go to original source...
    8. Jacoboni, A., Standardi, A.: Tissue culture of jojoba (Simmondsia chinensis, Link).-Acta Hort. 212: 557-560, 1987. Go to original source...
    9. Llorente, B., Apostolo, N.M.: Effect of different growth regulators and genotype on in vitro propagation of jojoba.-New Zeal. J. Crop Hort. Sci. 26: 55-62, 1998. Go to original source...
    10. Lloyd, G., McCown, B.: Commercially feasible micropropagation of mountain laurd (Kalmia latiflora) by use of shoot tip culture.-Proc. int. Plant Propag. Soc. 30: 421-427, 1980.
    11. Mills, D., Wenkart, S., Benzioni, A.: Micropropagation of Simmondsia chinensis (jojoba).-In: Bajaj Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 40. High-Tech and Micropropagation VI. Pp. 370-393. Springer-Verlag, Berlin 1997. Go to original source...
    12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    13. Rost, T.L., Hinchee, M.A.W.: Preliminary report of the production of callus, organogenesis and regeneration of jojoba (Simmondsia chinensis (Link) Schneider) in tissue culture.-J. hort. Sci. 55: 299-305, 1980. Go to original source...
    14. Roussos, P.A., Tolia-Marioli, A., Pantikis, C.A., Kotsias, D.: Rapid multiplication of jojoba seedlings by in vitro culture.-Plant Cell Tissue Organ Cult. 57: 133-137, 1999. Go to original source...
    15. Scaramuzzi, F., D'Aambrosio, A.: Organogenesis and propagation 'in vitro' of Simmondsia chinensis (Link) Schneider (jojoba) from vegetative fragments.-Acta Hort. 227: 411-413, 1988. Go to original source...
    16. Vooková, B., Kormuták, A.: Effect of sucrose concentration, charcoal and indole-3-butyric acid on germination of Abies numidica somatic embryos.-Biol. Plant. 44: 181-184, 2001. Go to original source...
    17. Weatherhead, M.A., Burdon, J., Henshaw, G.G.: Effect of activated charcoal as an additive plant tissue culture media.-Z. Pflanzenphysiol. 94: 399-406, 1979. Go to original source...

    Return to the content