Biologia plantarum 46:433-436, 2003 | DOI: 10.1023/B:BIOP.0000023889.20631.92

Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum

A.K. Banerjee1, D.C. Agrawal2,*, S.M. Nalawade2, S. Hazra2, K.V. Krishnamurthy2
1 Horticulture Hall, Iowa State University, Ames, USA
2 Plant Tissue Culture Division, National Chemical Laboratory, Pune, India

The report describes in vitro plant regeneration from embryo axis explants of six cultivars of cotton. Induction of a maximum number of multiple shoots in all six cultivars could be achieved on Murashige and Skoog's (MS) salts and Gamborg's (B5) vitamins supplemented with 0.4 μM benzyladenine (BA) and 0.1 μM napthaleneacetic acid (NAA). Elongated shoots could be rooted on half strength medium supplemented with 0.5 μM NAA. Rooted shoots survived (92 %) after hardening in the greenhouse and grew to maturity (100 %) after transfer to field.

Keywords: benzyladenine; cotton; micropropagation; napthaleneacetic acid
Subjects: auxins; cultivar and genotype differences, regeneration from embryo axes; cytokinins; embryo axis, regeneration; Gossypium hirsutum; in vitro culture, from embryo axes; in vitro culture, micropropagation; multiple shoot formation

Published: November 1, 2003Show citation

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Banerjee, A.K., Agrawal, D.C., Nalawade, S.M., Hazra, S., & Krishnamurthy, K.V. (2003). Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum. Biologia plantarum, 46(3), 433-436. doi: 10.1023/B:BIOP.0000023889.20631.92.
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