Biologia plantarum 46:445-448, 2003 | DOI: 10.1023/B:BIOP.0000023892.82238.f8

Molecular Characterization of Three Heritiera Species Using AFLP Markers

A.K. Mukherjee1,*, L.K. Acharya2, I. Mattagajasingh1, P.C. Panda2, T. Mohapatra3, P. Das1
1 DNA Fingerprinting Laboratory, Department of Plant Biotechnology, Regional Plant Resource Centre, Orissa, India
2 Department of Taxonomy and Conservation, Regional Plant Resource Centre, Orissa, India
3 DNA Fingerprinting and Rice Genome Laboratory, National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India

In the present study three species of Heritiera Aiton (Sterculiaceae) were characterized using 9 amplified fragment length polymorphism (AFLP) primer combinations and the genetic relationship among these three species was assessed, Nine AFLP primer combinations yielded 445 bands out of which 210 were monomorphic and 235 were polymorphic. Out of the 235 polymorphic bands 79 were present only in a single species. Among the total amplified bands 255 were shared between H. fomes and H. littoralis, 225 were shared between H. fomes and H. macrophylla and 306 bands were shared between H. littoralis and H. macrophylla. The cluster analysis showed H. littoralis is closer to H. macrophylla than H. fomes. The similarity between H. fomes and H. littoralis was higher than that of H. fomes and H. macrophylla. The present study indicates that H. littoralis is better classified as mangrove associate or back mangal than a true mangrove.

Keywords: cluster analysis; mangroves; monomorphic and polymorphic bands
Subjects: amplified fragment length polymorphism (AFLP); dendrogram; Heritiera fomes; Heritiera littoralis; Heritiera macrophylla; markers, genetic

Published: November 1, 2003Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Mukherjee, A.K., Acharya, L.K., Mattagajasingh, I., Panda, P.C., Mohapatra, T., & Das, P. (2003). Molecular Characterization of Three Heritiera Species Using AFLP Markers. Biologia plantarum, 46(3), 445-448. doi: 10.1023/B:BIOP.0000023892.82238.f8.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Ajmone Marsan, P., Castiglioni, P., Fusari, F., Kuiper, M., Motto, M.: Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers.-Theor. appl. Genet. 96: 219-227, 1998. Go to original source...
    2. Banerjee, L.K., Rao, T.A.: Mangroves of Orissa Coast.-Bishen Singh Mahendra Pal Singh Publishers, Dehra Dun 1990.
    3. Brown, J.K.M.: The choice of molecular markers methods for population genetic studies of plant pathogens.-New Phytol 133: 183-195, 1996. Go to original source...
    4. Das, A.B., Basak, U.C., Das, P.: Karyotype diversity in three species of Heritiera, a common mangrove tree on the Orissa Coast.-Cytobios 80: 71-78, 1994.
    5. Folkertsma, R.T., Rouppe van der Voort, J.N.A.M., De Groot, K.E., Van Zandvoort, P.M., Schots, A., Gommers, F.J., Helder, J., Bakker, J.: Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis.-Mol. Plant-Microbe Interact. 9: 47-54, 1996.
    6. Garcia-Mas, J., Oliver, M., Gomez-Paniagua, H., De Vicente, M.C.: Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon.-Theor. appl. Genet. 101: 860-864, 2000. Go to original source...
    7. Gherardi, M., Mangin, B., Goffinet, B., Bonnet, D., Huguet, T.: A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular markers.-Theor. appl. Genet. 96: 406-412, 1998. Go to original source...
    8. Hansen, M., Hallden, C., Nilsson, N.-O., Sall, T.: Markers assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers.-Mol. Breed. 3: 449-456, 1997. Go to original source...
    9. Henry, R.J.: Practical Applications of Plant Molecular Biology.-Chapman & Hall, London-Weinheim-New York-Tokyo-Melbourne-Madras 1997.
    10. Jaccard, P.: Nouvelles recherches sur la distribution florale.-Bull. Soc. Vaud. Sci. Nat. 44: 223-270, 1908.
    11. Karp, A., Edwards, K., Bruford, M., Vosman, B., Morgante, M., Seberg, O., Kremer, A., Boursot, P., Arctander, P., Tautz, D., Hewitt, G.: Newer molecular technologies for biodiversity evaluation: opportunities and challenges.-Nat. Biotechnol. 15: 625-628, 1997. Go to original source...
    12. Kartawinata, K., Adisoemarto, S., Soemodihardjo, S., Tantra, I.G.M.: Status Pengetahuan Hutan Bakau di Indonesia.-In: Soemodihardjo, S., Nontji, A., Djamali, A. (ed.): Proceedings of Seminar Ecosystem Hutan Mangrove. Pp. 21-39. LIPI, Indonesia 1979.
    13. Kostermans, A.J.G.J.: Monograph of the genus Heritiera Aiton (Sterant).-Reinwardtia 4: 465-583, 1959.
    14. Lerceteau, E; Szmidt, A.E.: Properties of AFLP markers in inheritance and genetic diversity studies of Pinus sylvestris L.-Heredity 82: 252-260, 1999. Go to original source...
    15. Maguire, T.L., Peakall, R., Saenger, P.: Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh. (Avicenniaceae) detected by AFLPs and SSRs.-Theor. appl. Genet. 104: 388-398, 2002. Go to original source...
    16. Maughan, P.J., Saghai Maroof, M.A., Buss, G.R., Huestis, G.M.: Amplified fragment length polymorphism (AFLP) in soybean: species diversity, inheritance, and near-isogenic line analysis.-Theor. appl. Genet. 93: 392-401, 1996. Go to original source...
    17. Naskar, K.R., Mandal, R.N.: Ecology and Biodiversity of Indian Mangroves. Vol. 1 & II.-Daya Publishers, New Delhi 1999.
    18. Parani, M., Lakshmi, M., Elango, S., Ram, N., Anuratha, C.S., Parida, A.: Molecular phylogeny of mangroves II. Intra and inter-specific variation in Avicennia revealed by RAPD and RFLP markers.-Genome 40: 487-495, 1997. Go to original source...
    19. Parani, M., Lakshmi, M., Senthilkumar, P., Ram, N., Parida, A.: Molecular phylogeny of mangroves. V. Analysis of genome relationships in mangrove species using RAPD and RFLP markers.-Theor. appl. Genet. 97: 617-625, 1998. Go to original source...
    20. Ranade, S.A., Verma, A., Gupta, M., Kumar, N.: RAPD profile analysis of betel vine cultivars.-Biol. Plant. 45: 523-527, 2002. Go to original source...
    21. Rohlf, F.J.: NTSYS-PC: numerical taxonomy and multivariate analysis system.-Exeter Software, New York 1997.
    22. Saghai-Maroof, M.A., Soliman, K.M., Jerenson, R.A., Allard, R.W.: Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics.-Proc. nat. Acad. Sci. USA 81: 8014-8018, 1984. Go to original source...
    23. Santisuk, T.: Taxonomy of the terrestrial trees and shrubs in the Mangrove formation in Thailand.-In: The First UNDP/UNESCO Regional Training Course on Introduction to Mangrove Ecosystem. National Research Council, Bangkok 1983.
    24. Skroch, P., Nienhuis, J.: Impact of scoring error and reproducibility of RAPD data on RAPD based estimates of genetic distance.-Theor. appl. Genet. 91: 1086-1091, 1995. Go to original source...
    25. Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy.-Freeman, San Francisco 1973.
    26. Tomlinson, P.B.: The Botany of Mangroves.-Cambridge University Press, Cambridge 1986.
    27. Vos. P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M.: AFLP: a new technique for DNA fingerprinting.-Nucl. Acids Res. 23: 4407-4414, 1995. Go to original source...

    Return to the content