Biologia plantarum 50:417-420, 2006 | DOI: 10.1007/s10535-006-0059-4

Regeneration via organogenesis in callus cultures of Argyrolobium roseum

P. K. Khanna1, A. Ahuja1,*, M. Sharada1, G. Ram1, K. Koul1, M. K. Kaul1
1 Biodiversity and Applied Botany Division, Regional Research Laboratory (CSIR), Jammu-Tawi, India

A reproducible protocol has been developed for high frequency plant regeneration from immature embryos of Argyrolobium roseum Jaub & Spach, an important medicinal legume. Green nodular calli were initiated from immature embryos excised from 10-d-old pods in 70 % of cultures within 3 weeks when grown on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm-3 benzylaminopurine (BAP) + 0.25 mg dm-3 indole-3-acetic acid (IAA). Subsequent transfer of 5 mm2 callus pieces to MS medium supplemented with BAP (0.5 mg dm-3) alone or in combination with IAA (0.25 mg dm-3) facilitated regeneration of multiple shoots. Organogenic calli bearing multiple shoots when transferred to MS medium supplemented with BAP (0.5 mg dm-3) + IAA (0.25 mg dm-3) supported rapid shoot elongation. Shoot propagules subcultured to Gamborg's medium (B5) with 0.5 mg dm-3 indole-3-butyric acid (IBA) rooted with 80 % frequency and developed into phenotypically normal plants. Plantlets were successfully acclimatized in a sterile mixture of sand and garden soil (1:1) under greenhouse and thereafter transferred to field beds.

Keywords: BAP; 2,4-D; IAA; IBA; immature embryo; KIN; legume; medicinal plant; NAA
Subjects: Argyrolobium roseum; auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; medicinal plants; nutrient medium, Murashige and Skoog; root, rooting

Received: August 18, 2004; Accepted: May 24, 2005; Published: September 1, 2006Show citation

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Khanna, P.K., Ahuja, A., Sharada, M., Ram, G., Koul, K., & Kaul, M.K. (2006). Regeneration via organogenesis in callus cultures of Argyrolobium roseum. Biologia plantarum, 50(3), 417-420. doi: 10.1007/s10535-006-0059-4.
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