biologia plantarum

Biologia plantarum 50:763-766, 2006 | DOI: 10.1007/s10535-006-0126-x

Proline accumulation in two bean cultivars under salt stress and the effect of polyamines and ornithine

J. F. Jiménez-Bremont^1,*, A. Becerra-Flora¹, E. Hernández-Lucero¹, M. Rodríguez-Kessler¹, J. A. Acosta-Gallegos², J. G. Ramírez-Pimentel³

¹ División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Tangamanga, México

² Programa de Frijol, Campo Experimental Bajío-INIFAP, AP 312, Celaya, México

³ Subdirección de Investigación y Graduados Agropecuarios, Instituto Tecnológico Agropecuario No. 33, K8 Carretera Celaya-Juventino Rosas, AP 508, Celaya, México

Proline accumulation in two different bean (Phaseolus vulgaris L.) cultivars, one drought-sensitive (Canario 60) and one drought-resistant (Pinto Villa) was investigated. Both tolerated salt concentrations up to 150 mM NaCl, but the sensitive Canario 60 did not survive at 400 mM NaCl. In response to salt stress, both cvs. accumulated proline in all the analyzed tissues, the lowest contents were detected in roots. Pinto Villa accumulated higher proline concentrations than Canario 60 only at 400 mM NaCl. The addition of polyamines or ornithine increased proline content in plant tissues without stress, while they decreased it under salt stress.

Keywords: Phaseolus vulgaris; putrescine; salt tolerance; spermidine; spermine

Subjects: bean; cultivar and genotype differences; Phaseolus vulgaris; polyamines; proline; putrescine; salt, salinity stress; spermidine; spermine

Received: February 10, 2005; Accepted: September 19, 2005; Published: December 1, 2006Show citation