Biologia plantarum 53:505-510, 2009 | DOI: 10.1007/s10535-009-0091-2

Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves

B. R. Lee1, L. S. Li1, W. J. Jung2, Y. L. Jin3, J. C. Avice4, A. Ourry4, T. H. Kim1,*
1 Department of Animal Science, Environmental-Friendly Agriculture Research Center, Chonnam National University, Gwangju, Korea
2 Department of Biological and Environmental Chemistry, Environmental-Friendly Agriculture Research Center, Chonnam National University, Gwangju, Korea
3 College of Plant Protection, Qingdao Agriculture University, Qingdao City, Shandong, P.R. China
4 Institut de Biologie Fondamentale et Appliquée, Université de Caen, Caen Cedex, France

The objective of this study was to determine the development of the antioxidant enzymes induced by drought stress in white clover (Trifolium repens L.) leaves. Water stress was imposed during 28 d by decreasing the daily irrigation. Leaf water potential (Φw) gradually decreased from -0.46 to -2.33 MPa. For the first 7 d, dry mass (DM), H2O2 and lipid peroxidation were not significantly affected by water deficit. From 14 d of treatment, water stress decreased dry mass and increased content of reactive oxygen species (O2 .- and H2O2) and oxidative stress (malondialdehyde content). The ascorbate peroxidase (APOD) was activated most rapidly, already during the first week of water stress, but then its activity slowly decreased. Activation of superoxide dismutase (SOD) and catalase (CAT) by water deficit continued during the 14 d (Φw ≥ -1.65 MPa) and then their activities remain on the similar level. The activity of guaiacol-peroxidase (GPOD) increased mostly under progressive water stress and was correlated with increase in lipid peroxidation and growth restriction.

Keywords: ascorbate peroxidase; catalase; drought; leaf water potential; guaiacol peroxidase; superoxide dismutase; Trifolium repens
Subjects: ascorbate peroxidase; catalase; guaiacol peroxidase; hydrogen peroxide; malondialdehyde; relative water content (RWC); superoxide; superoxide dismutase (SOD); Trifolium repens; water potential; water stress; white clover

Received: October 17, 2007; Accepted: April 5, 2008; Published: September 1, 2009Show citation

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Lee, B.R., Li, L.S., Jung, W.J., Jin, Y.L., Avice, J.C., Ourry, A., & Kim, T.H. (2009). Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves. Biologia plantarum, 53(3), 505-510. doi: 10.1007/s10535-009-0091-2.
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